Posted by admin on Nov 30, 2019 in |
Objectives: The aim of this work was the preparation of Clotrimazole (CM) loaded nanostructured lipid carriers (CM-NLC) and the examination of in-vitro antifungal activity of prepared formula. Methods: High shear hot homogenization method was used for CM-NLC preparation. Different ratios of Compritol AT 888 and Labrasol were used and combined with two types of surfactants: Kolliphor P188 or Kolliphor EL to stabilize NLC dispersion. The prepared formulae were tested for diameter, zeta potential, loading capacity, in-vitro release, differential scanning calorimetry (DSC), Transmission electron microscopy (TEM) and also for antifungal activity against strains of Candida albicans. Results: six formulae were successfully prepared with Z-average diameter ranges from 468.9 ± 3.7 down to 18.63 ± 0.964 nm, and high loading capacity of the drug (˃50%) and Polydispersity index less than 1, indicating homogeneity of size. DSC images ensured drug solubility in the lipid matrix and dispersed in NLC in an amorphous state. While TEM images ensured that the CM-NLC formulation had a size below 30 nm and a spherical shape. In-vitro...
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Posted by admin on Nov 30, 2019 in |
Objectives: The main objective was to find out a suitable and effective natural release retardant, which can be used in matrix tablet preparation along with synthetic excipients. Methods: Tamarind seed polysaccharides (TSP) were isolated from Tamarindus indica by hot extraction method followed by freeze-drying and yield was found to be 16.85%. Microbial studies confirmed that TSP doesn’t support microbial growth. Matrix tablet of Metformin HCl was prepared by using a wet granulation method with the help of TSP. Drug excipients compatibility was checked with the help of Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). At last, the release was compared with the marketed formulation. Results: The drug content of all the formulations ranged from 96.55% to 98.54%. Formulation F7 showed release up to 12 h. It was observed that as per increase in the polymer concentration, the release rate also increased. F6 formulation was found to be the optimized formulation. The F6 and marketed both the formulation followed the Higuchi model as n values came within...
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Posted by admin on Nov 30, 2019 in |
Nepafenac is an analgesic and anti-inflammatory drug, used in post-operative ocular pain and presently available in the market in the form of 0.1% ophthalmic suspension eye drop due to aqueous insolubility of the drug. The present formulation is not only poor patient compliant due to suspension form but also suffers from a short corneal residence and inadequate ocular bioavailability. The objective of the present research work is to enhance the aqueous solubility of lipophilic drug nepafenac and develop its ocular gel formulation for overcoming the drawbacks of the presently marketed product. The 10% w/v HP-β-CD solution was used to formulate nepafenac in solution form, which resulted in 57.14 fold solubility enhancement of nepafenac in water, facilitating the development of nepafenac eye drop-in solution form. To enhance the pre-corneal residence of the formulation, the drug solution was incorporated into the hydrogel base, i.e. gellan gum capable of ion-activated sol-to-gel transformation in the ocular environment while sodium hyaluronate forms gel that exists in slightly viscous flowable form. The developed formulation was...
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Posted by admin on Nov 30, 2019 in |
Background: Sida spinosa (Family: Malvaceae) is a well-known herb, has considerable medicinal significance in the tribal region of Chhattisgarh. Several ethnomedicinal survey reports have been revealed its potential to cure ailments such as skin diseases, diarrhea, gonorrhea, and asthma. Objective: The aim of the present study to screen phytochemical, flavonoid content, antioxidant and anthelmintic potential of Sida spinosa. Methods: Aqueous and ethanolic extracts of Sida spinosa leaves were prepared by the Soxhlet method. The extracts were phytochemically analyzed for alkaloid, flavonoid, tannin, carbohydrate, glycoside, and Phytosterols. Extracts were also tested for flavonoid content, antioxidant potential, and anthelmintic activity using Indian adult earthworm (Pheretima posthuma). Results: The phytochemical screening confirmed the presence of alkaloids, carbohydrates, glycosides, flavonoids, steroids, in the leaves of Sida spinosa. The Present study also reported that ethanolic extract has exhibited significant free radical scavenging capacity and anthelmintic activity as compared to aqueous extract. Conclusion: The present study has evidenced the prominent antioxidant and anthelmintic action of Sida spinosa, which may contribute to the development of an...
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Posted by admin on Nov 30, 2019 in |
In this study we aimed at isolation, identification, and quantification of two phenolic compounds reported for the first time in leaves of Amaranthus hypochondriacus. A non targeted GC-MS metabolite profiling of hexane extracts of amaranth leaves was done which indicated the presence of major phenolic compounds. Two alkylated phenols namely 2,4-di-tert-butyl phenol (A) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) were isolated for the first time from the hexane extract of A. hypochondriacus leaves. Structure elucidation of both the compounds was done using 1H NMR and purity was checked by GC-MS. Quantitative estimation of purified compounds in leaves extract was carried out using RP-analytical HPLC. The antioxidant potential of the isolated compounds (A), (B) and the hexane extract of leaves were investigated using in-vitro 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radicals scavenging bioassay. Hexane leaf extract (IC50 = 385.34 ± 37.11 µg/mL), 2,4-di-tert-butyl phenol (A) (IC50 = 253.76 ± 24.67 µg ml-1) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) (IC50 = 174.09 ± 16.89 µg ml-1) exhibited significant free radicals scavenging activity. Thus, our study reports...
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