Posted by admin on Oct 31, 2019 in |
Objective: To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were extracted with methanol. Methanolic extract prepared separated with n-butanol, chloroform, and ethyl acetate as total saponin, total alkaloid and phenolic compound fractions respectively. The chemical characterization of oleanolic acid was done by High-Performance Thin Layer Chromatography (HPTLC) in a mixture of toluene: methanol (9:1) as a mobile phase which developed in anisaldehyde sulphuric acid reagent. Result: The extraction yield of total saponin fraction was 4.3% (w/w). The Rf value 0.31 found to same as the value of standard oleanolic acid. Linear regression analysis data for the calibration plots showed a good linear relationship between concentration (1-6 μg) R (r2) = 0.992408 concerning peak height and area. The limit of detection and limit of quantification of Oleanolic acid were detected to be 10.75 μg and 40.70 μg per spot. The amount of oleanolic acid...
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Posted by admin on Oct 31, 2019 in |
Taraxacum officinale (TO) Weber commonly known as dandelion has contained triterepenes-taraxasterol and taraxerol in its roots which are nowadays being recognized as potent anticancer compounds. The main objective behind the present study was to increase the yield of taraxasterol and taraxerol through induction of hairy roots in Taraxacum officinale Weber using two strains of Agrobacterium rhizogenes MTCC- 532 and MTCC-2084. An efficient protocol for the induction of hairy roots is proposed from the root callus cultures of Taraxacum officinale maintained on MS + IAA + BA + 2, 4-D (0.5 ppm + 1 ppm + 0.5 ppm) through infection of the root callus with two strains of Agrobacterium rhizogenes MTCC-532 and MTCC-2084. It was found that MTCC-532 proved to be effective in hairy roots induction whereas MTCC-2084 failed to induce hairy roots. HPLC results showed the retention time of taraxerol and taraxasterol at 4.975 and 3.5 min respectively. Showing fourfold increase in content of taraxasterol and a nearly two-fold increase in...
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Posted by admin on Oct 31, 2019 in |
Dissolution testing with biorelevant media is used in the pharmaceutical industry as a predictive tool for the estimation of drug formulation’s in-vivo performance in bioequivalence studies. The objective of this study was to determine the biorelevant dissolution media enabling the prediction of in-vivo performance of Valsartan and Chlorthalidone in solid oral dosage form as new fixed drug combination. Dissolution profiles were performed in different pH medias for the evaluation of Valsartan/Chlorthalidone film-coated tablets. The validation studies of the used dissolution method were conducted by performing the parameters specified in the ICH (The International Conference on Harmonization) Q2 (R1) Guideline and the results met the acceptance criteria. Although in-vitro dissolution test findings showed the similarity of release profile of test and mono reference drug products, the in-vivo results demonstrated that they are not similar. For this reason, biorelevant media was investigated and defined. This media proved to be biorelevant and has the potential to be further used to establish in-vitro in-vivo correlation (IVIVC) during the development of Valsartan and Chlorthalidone...
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Posted by admin on Oct 31, 2019 in |
Melastoma malabathricum is a medicinal plant found throughout Southeast Asian countries. Traditionally M. malabathricum was used for treating diseases such as hemorrhoids, cuts and wounds, toothache, and stomachache. Scientific findings also revealed the wide pharmacological actions of various parts of M. malabathricum such as anti-nociceptive, anti-inflammatory, wound healing, anti-diarrheal, cytotoxic, and antioxidant activities. The present study evaluated the mechanism of action of the anti-inflammatory activity of M. malabathricum. Initially different solvent extracts of M. malabathricum were evaluated for total phenolics, total flavonoids, anti-oxidant activity, anti-inflammatory activity (protein denaturation), anti-cancer activity (prostate cancer cell line), and for sPLA2 inhibition. Results showed that ethanolic extract of Melastoma. malabathricum leaves showed higher phenolic and flavonoid concentrations, i.e. 36.77mg GAE/g dry wt. and 245.5 mg QE/gm of dry weight, respectively. Ethanol extract also exhibited the highest free radical scavenging activity in the DPPH method and phosphomolybdenum assay of which the IC50 values were 77.424 µg/ml and 95.159 µg/ml respectively. The anti-inflammatory assay (protein denaturation assay) of leaf extract showed the IC50 value of 45.518 µg/ml. Further, the extract exhibited...
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Posted by admin on Oct 31, 2019 in |
Pharmacists play a vital role in providing counseling to the hypertensive patients to understand disease, risk factors, medications, and non-pharmacological measures required in controlling Blood Pressure (BP). This study aims to evaluate the impact of pharmacist delivered counseling services on KAP levels and control of BP in hypertensive patients. A prospective, open-labeled, randomized control trial was conducted among hypertensive patients attending medical outpatient department of Non-governmental Organization (NGO) Hospital located in rural settings in Anantapur district, Andhra Pradesh, India. The study was performed after getting ethical clearance from the Institutional Review Board with a registration number of RIPER/IRB/2018/038 and in accordance with ICH-GCP and CONSORT guidelines. The study was carried out for a period of one year from February 2018 to January 2019. The findings of the study reveal that there was a significant difference in the mean systolic and diastolic BP levels in intervention (138 ± 10.5, 82.3 ± 7.2) and control group (146.0 ± 8.2, 87.2 ± 5.4) at final follow-up visits. The mean percentage of knowledge,...
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