Posted by admin on Dec 31, 2019 in |
The research herbal formulation prepared by mixing dried stem barks of Azadirachta indica A. Juss. and Saraca asoca Roxb. in equal amounts is standardized through pharmacognostic and phytochemical studies for assessing its efficacy in the treatment of leucorrhoea/vaginitis/ excessive white discharge. Pharmacognostical analysis revealed total ash value of 9.43% having 1.89% acid insoluble ash, 4.62 pH and 7.70% moisture content and presence of nitrogen and halogens. Flavonoids, tannins, carbohydrates, glycosides, saponins and fixed oils were found present in the hydro-alcoholic extracts. The results also showed a high concentration of flavonoids compounds (43.37 µg quercetin equivalent/mg) and high phenolic content (101.22 µg gallic acid equivalent/mg). UV-Visible spectroscopy scanning showed a prominent peak at 277 nm in the hydro-alcoholic extract. HPTLC analysis at 254 nm, 277 nm, and 330 nm indicated the presence of tannic acid, gallic acid, quercetin and catechol. Similarly, HPLC analysis at 277 nm suggested the presence of gallic acid, tannic acid and catechol. FTIR analysis exhibited the presence of N-H stretching, C-H stretching, N-H bending, N-O asymmetric...
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Posted by admin on Dec 31, 2019 in |
Analytical method for simultaneous estimation of Ertugliflozin (ERT) and Metformin hydrochloride (MET) was developed and validated by high-performance liquid chromatography (HPLC) as per ICH guidelines. The drugs were injected into the inertsil C18 (250 × 4.6 mm) maintained at room temp and wavelength 220 nm. The mobile phase consists of buffer (potassium dihydrogen pH 4.0) and methanol (65:35 v/v). The flow rate is maintained at 1.0 mL/min. The calibration curve was linear and regression coefficient (R2) value was found to be 0.999 and concentration ranging from 1.5-4.5 µg/mL and 100-300 µg/mL for Ertugliflozin and Metformin hydrochloride respectively. The LOD and LOQ of the method were found 1.04 μg/mL, 9.61 µg/mL and 0.0007 µg/mL, 0.006 µg/mL for Ertugliflozin and Metformin HCl. The developed method was found to be simple, precise, specific, linear and accurate as validated as per USP and International Conference on Harmonization (ICH)...
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Posted by admin on Dec 31, 2019 in |
Arnebia benthamii (synonym – Microtomia benthamii) commonly known as Ratanjot or Laljari found in western Himalayan region and used as wound healer roots by local vaids and tribes. The roots of Arnebia benthamii contain a red dye that is used in various health disorders like fungal infection, inflammation, fever and coloring/flavoring agent in Indian curries. To estimate wound healing potential of root extract of Arnebia benthamii, percentage wound contraction, wound area, epithelization time, tensile strength and wound index were measured in 13 days of wound healing study. The results showed highly significant wound healing in excision as well as incision wound healing models (P<0.01). The Arnebia benthamii extract-treated wounds showed about 41% higher wound contraction rate and 44% increased tensile strength in comparison with negative control animals. The epithelization time decreased by 43% and wound index value decreased by 89% in comparison to negative control animals which indicated that methanolic root extract of Arnebia benthamii is very helpful in faster and high-quality wound healing (P <...
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Posted by admin on Nov 30, 2019 in |
The research work focused on the optimization of Terbinafine HCl (TBH) pluronic lecithin organogels (PLOs) for increasing the penetration and efficacy of the drug. The factors affecting the PLOs such as viscosity and percentage drug release, were screened and optimized using the Box-Behnken design. The optimum formulation based on desirability (0.995) exhibited 3139.92 Cp for viscosity and 93.4561% for drug release. The optimized PLO was evaluated for drug content, pH, in-vitro drug release and ex-vivo studies. The percentage cumulative release of TBH permeated from optimized PLOs and marketed cream (MC) at the end of 12 h after the application was found to be 60.29 ± 0.69% and 19.35 ± 2.19% respectively. The comparatively higher permeability TBH in optimized PLOs could be due to increased solubility and diffusion coefficient of the drug. In-vitro microbial assay, studies showed that the PLO reduced the fungal burden of Candida albicans as compared to M.C (0.34 ± 0.08 mm) in a shorter duration of time with a larger zone of inhibition (0.58 ± 0.12...
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Posted by admin on Nov 30, 2019 in |
This study aimed to investigate the preparation and characterization of polymeric nanoparticles containing extract of Andrographis paniculata using Eudragit S 100 polymer. The methanolic extract of aerial parts of Andrographis paniculata Nees (Acanthaceae) was prepared and standardized for andrographolide content using the High-Performance Liquid chromatography technique. Three formulations containing 50, 100 and 150 mg of extract were prepared by solvent displacement method. Parameters including drug: polymer ratio, stabilizer, solvent, and stirring speed were optimized. Characterization of the formulations was carried out using particle size analysis, PDI, DSC, X-RD, SEM techniques, entrapment efficiency and in-vitro drug release studies. The particle size of the nanoformulations was found to be within the range of 300-400 nm, with entrapment efficiency more than 75% and drug release more than 55% after 8 h. Aerial parts extract of Andrographis paniculata was embedded and dispersed in Eudragit S 100 polymer as smooth spherical nanoparticles which may prove to be a novel drug delivery...
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