Posted by admin on Sep 30, 2019 in |
The objective of this study was to evaluate the toxicity of a saponin isolated from the fruits of the Momordica dioica as per OECD guidelines. Saponin was isolated from the methanolic extract of the fruits of M. dioica, and acute, sub-acute, sub-chronic and chronic toxicity was evaluated. The study of the acute toxicity of saponin M. dioica (SMD) at a single dose of 5000 mg/kg/b.w. by oral route in, recorded no mortality, and showed no change in the general behavior of the treated rats for 14 days. Administration at the doses; 1000, 500, and 250 mg/kg/b.w. by oral route in rats for 28 days and an animal showed doses of 500 and 250 mg/kg/b.w. of SMD was safe, no mortality for sub-acute but in a repeated dose of 1000 mg/kg/b.w. group produced treatment-related signs of toxicity. The study of Sub-chronic toxicity at the doses; 500, 250 and 100 mg/kg/b.w. of SMD in rats for 90 days and animals showed normal; hematological and biochemical parameters and gross findings compared to...
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Posted by admin on Sep 30, 2019 in |
Though at present, Post-Traumatic Stress Disorder (PTSD) stands to be one of the major mental debilitating psychiatric disorders, however, its clinical diagnosis remains unachieved due to the absence of any biological marker. Hence, this study is aimed at the identification of putative biological underpinnings of PTSD through the metabonomic approach. For this purpose, the animal model based NMR spectroscopy approach was undertaken for profiling of urine samples, for classification of metabolic changes brought about in body due to PTSD. Multivariate statistical analysis techniques, namely Principal Component Analysis (PCA) and Partial Least Square Discriminant Analysis (PLS-DA) were performed on 1H NMR spectral data, which was obtained from both rat urine sets that are, before and after exposing the rats to Underwater Trauma (UT) for PTSD. The behavioral changes were measured and recorded by conducting the Open Field Test (OFT). PCA showed partial separation with PC1 = 49.8% and PC2 = 21.7%, however PLSDA completely separated both urine sets with R2 = 0.9 and Q2 = 0.8. Eight endogenous metabolites were...
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Posted by admin on Sep 30, 2019 in |
In this study, in-situ raft forming Levofloxacin Suspension formulation was developed. In-vitro conditions like temperature, pH, and RPM simulating the in-vivo conditions like gastric pH, body temperature, and gastric motility respectively were identified as critical process parameters in system scale-up studies. Challenging and characterization were performed in-vitro. The working limits were identified and verified by calculation of process capability indices. Process Potential (Cp) and Process performance (Cpk) values greater than 2, and 1.33 respectively helped to select the conditions for the formation of raft system controlling the release up to 8 h with zero order release kinetics found to be significant (R2=0.9930). The release mechanism was found to be Korsemeyer-peppas showing the mechanism of drug release by diffusion and relaxation of polymeric raft structure. The Suspension formulation subjected to stability studies (40 ºC ± 2 ºC / 75% ± 5% RH) were found to be stable for pH, viscosity, floating lag time, content uniformity and percentage drug release from the raft structure. Moreover, radiographic x-rays evaluation for optimized formulation...
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Posted by admin on Sep 30, 2019 in |
Background: Garlic has been used in world culinary art as well as in herbal medicine since long back. Allicin, an active moiety in garlic, is truly recognized as heart of garlic. But allicin is highly unstable and undergoes formation of numbers of sulfur-containing compounds with reduced allicin content. The major problem associated with dosage form development is instability of allicin. Objective: The aim of the present research work was to obtain a stable garlic powder from the garlic bulbs with maximum allicin content with prolonged physical and chemical stability of the garlic powder. The target was to improve household storage for culinary purpose as well increased active drug content proclaimed for long term utilization in dosage form design. Methodology: The principles of microwave drying technology and the flower drying technology along with some modifications have been successfully utilized for preparing, processing and preserving garlic powder to improve its medicinal and culinary acceptance. Results: The allicin content was found to be increased (2533.7 µg/gm) as compared aqueous garlic extract and...
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Posted by admin on Sep 30, 2019 in |
The present research work was carried out to Formulate and evaluation of bilayer tablet dosage form for the treatment of Hypertension. The objective of this study to compare the specific characteristics of Olmesartan Midoxomil [Angiotensin II receptor antagonist] and Hydrochlorothiazide [Thiazide Diuretics] in order to design stable formulation. It can be concluded that Olmesartan Midoxomil [Angiotensin II receptor antagonist] and Hydrochlorothiazide [Thiazide Diuretics] were successfully formulated in combination as a Bilayer tablet form with Croscarmellose sodium, Lactose monohydrate and microcrystalline cellulose PH101 for immediate release of both drugs. Both drugs were found to be stable in Bilayer tablet formulation and were found to be stable up to 6 months. This bilayer tablet dosage form increases the stability which may reduce loss and cost of formulation. It improves the benefits of producer, retailer, and patients. Recently, greater attention has been focused on development of bilayer tablet formulations. Over the past 30 years, the expenses and complications involved in marketing new drug entities have increased with concomitant recognition of therapeutic advantages...
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