Posted by admin on Nov 30, 2019 in |
Background: Sida spinosa (Family: Malvaceae) is a well-known herb, has considerable medicinal significance in the tribal region of Chhattisgarh. Several ethnomedicinal survey reports have been revealed its potential to cure ailments such as skin diseases, diarrhea, gonorrhea, and asthma. Objective: The aim of the present study to screen phytochemical, flavonoid content, antioxidant and anthelmintic potential of Sida spinosa. Methods: Aqueous and ethanolic extracts of Sida spinosa leaves were prepared by the Soxhlet method. The extracts were phytochemically analyzed for alkaloid, flavonoid, tannin, carbohydrate, glycoside, and Phytosterols. Extracts were also tested for flavonoid content, antioxidant potential, and anthelmintic activity using Indian adult earthworm (Pheretima posthuma). Results: The phytochemical screening confirmed the presence of alkaloids, carbohydrates, glycosides, flavonoids, steroids, in the leaves of Sida spinosa. The Present study also reported that ethanolic extract has exhibited significant free radical scavenging capacity and anthelmintic activity as compared to aqueous extract. Conclusion: The present study has evidenced the prominent antioxidant and anthelmintic action of Sida spinosa, which may contribute to the development of an...
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Posted by admin on Nov 30, 2019 in |
In this study we aimed at isolation, identification, and quantification of two phenolic compounds reported for the first time in leaves of Amaranthus hypochondriacus. A non targeted GC-MS metabolite profiling of hexane extracts of amaranth leaves was done which indicated the presence of major phenolic compounds. Two alkylated phenols namely 2,4-di-tert-butyl phenol (A) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) were isolated for the first time from the hexane extract of A. hypochondriacus leaves. Structure elucidation of both the compounds was done using 1H NMR and purity was checked by GC-MS. Quantitative estimation of purified compounds in leaves extract was carried out using RP-analytical HPLC. The antioxidant potential of the isolated compounds (A), (B) and the hexane extract of leaves were investigated using in-vitro 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radicals scavenging bioassay. Hexane leaf extract (IC50 = 385.34 ± 37.11 µg/mL), 2,4-di-tert-butyl phenol (A) (IC50 = 253.76 ± 24.67 µg ml-1) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) (IC50 = 174.09 ± 16.89 µg ml-1) exhibited significant free radicals scavenging activity. Thus, our study reports...
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Posted by admin on Nov 30, 2019 in |
Objective: The present study was designed for Isolation of phytoconstituents from pharmacologically potent extracts of leaves of Cassia uniflora based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of leaves, stems, and fruits of cassia uniflora were prepared using various solvents such as water, methanol and hydro alcohol. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. These active extracts were subjected to column chromatography by means of different mobile phases followed by TLC. The isolated compounds were subjected to IR, 1H NMR, 13C NMR, and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of leaf of Cassia uniflora was found to be potent when compared with other extracts. This extract was subjected to column chromatography to get fractions and eluted fractions were run in TLC mobile phase with different solvent ratios. The fractions with similar Rf values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be Methyl inositol, Luteolin,...
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Posted by admin on Nov 30, 2019 in |
Delonix elata (D. elata) whole plant was chosen to evaluate in-vitro anti-oxidant activities. The total polyphenols and flavonoids were determined after the extraction. DPPH (2, 2′-diphenyl-1-picrylhydrazy), H2O2 (Hydrogen peroxide), NO (Nitric oxide) and TBARS (Thiobarbituric acid) methods were used to evaluate antioxidant activities of D. elata whole plant. The results showed that the highest levels of polyphenols were recorded in DEM (15.32 ± 0.32 mg of GA/g of extract). Total flavonoid contents of extracts/fractions showed that DEM (15.57 ± 0.69 mg of Qu/g of extract) contains the highest level. The DEM extract shows more inhibition of DPPH (92.56), NO (143.88), H2O2 (182.28) and TBARS (121.94 µg/ml), respectively when compared with other extracts. These findings suggest that the extracts obtained from D. elata stem have active substances contributing to the increase in natural antioxidant potential. It can be concluded from the findings that D. elata extracts have potent antioxidant...
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Posted by admin on Nov 30, 2019 in |
Objective: To explore the Teratogenicity of isolated Polyphenols of Symphyocladia latiuscula (PSL) on pregnant female rats. Experimental Design: The marine alga Symphyocladia latiuscula were the preparation of methanolic extract followed by isolation of Polyphenols. The Teratogenic study was performed according to the OECD guidelines, no 415. And the test drug; PSL 100, 200 and 1000 mg/kg and positive control Valproic acid (VPA); 1000 mg/kg was administered from 6th to 17th day of gestation during the period of fetal organogenesis. All females were killed on the 20th day of pregnancy, and their uterus was dissected to examine the Gross Abnormalities. Results and Conclusion: VPA significantly reduced all body measurements in terms of growth retardation, body weight, height, lower and upper limb, VPA showed mortality of 2 pups in the concentration of 1000 mg/kg/bw i.v whereas in PSL no significant differences were noted concerning the positive control group. The highest difference was shown especially PSL 1000 mg/kg/bw fetal morphological abnormalities; regarding the weight and length of the fetuses, there was a...
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