Posted by admin on Oct 31, 2019 in |
A simple and reliable head space Gas Chromatographic method has been developed for the determination of residual ethanol, isopropyl alcohol and toluene in Amiodarone hydrochloride drug substance. The proposed method is based on flame ionization detection technique with DB 624 as stationary phase. Linearity of detector response was established up to 150 % of the limit level for ethanol, isopropyl alcohol and toluene and the quantitation limit was 80 ppm for ethanol, 40 ppm for isopropyl alcohol and 4 ppm for toluene respectively. Performance of the method was assessed by evaluating the recovery, repeatability, reproducibility, linearity and limits of detection and quantification. The proposed method has a potential for application to drug substances which may contains traces of residual solvents. Results prove that the validated method was suitable for determining the residual ethanol, isopropyl alcohol and toluene in Amiodarone hydrochloride drug...
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Posted by admin on Oct 31, 2019 in |
Four isatin mixed ligand complexes of the type ML1L2, where M =Co(II), Ni(II), Cu(II) and Zn(II) (LI = Schiff base formed by the condensation of isatin and p-nitroaniline and L2= Schiff base formed by the condensation of isatin and 2,4-dinitroaniline) are synthesized. Mixed ligand complexes are different from routine metal complexes that they have a minimum of two special kinds of ligands linked to the one metal ion in a complex. Ethanol is used as a solvent for the synthesis of ligands and their mixed ligand complexes. All the mixed ligand complexes have been characterized by various physicochemical techniques such as Elemental analysis, IR, UV-Visible, conductivity measurements, magnetic moment measurements, thermogravimetric analysis, etc. Tetrahedral geometry is assigned for all the mixed ligand complexes. Here both the ligands are bidentate and potent donors are carbonyl oxygen and azomethine nitrogen atoms. The ligands and the mixed ligand complexes are screened for cytotoxicity and antioxidant properties. The mixed ligand metal complexes show higher antioxidant and cytotoxic activities as compared to the...
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Posted by admin on Oct 31, 2019 in |
The desire of the present study was to investigate in-vitro antimicrobial activity on different crude extracts of Ziziphus rugosa pericarp. The antibacterial assay was carried out against Bacillus subtilis MTCC 1133, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 1692 and Salmonella typhi ATCC 19430. Meanwhile, Candida albicans ATCC 18804, Aspergillus niger ATCC 16404, Trichoderma harzianum MTCC 3832 and Polyporus rubidus MTCC 140 were used for the antifungal assay. Both activities were carried out by cup plate method using 100, 200, and 300mg/ml of hexane, ethanol, and aqueous extracts. The hexane extract showed MIC at 100 mg/ml for B. subtilis, 60 mg/ml for S. aureus and S. typhi, and 80 mg/ml for E. coli. The ethanolic extract showed 80 mg/ml for all tested organisms. The aqueous extract showed 80, 40, 60, and 100 mg/ml for B. subtilis, S. aureus, E. coli, and S. typhi, respectively. Among the extracts, aqueous extracts showed highest level activity against both gm +ve (B. subtilis 26mm) and gm -ve (S. typhi 24mm) at 300 mg/ml....
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Posted by admin on Oct 31, 2019 in |
Tuberculosis is a global problem. The tuberculosis is the common cause of male infertility in developing countries due to the use of second-line anti-tuberculosis drugs (ATB). The experiments were conducted at Unique Bio diagnostic Enterprise Veterinary Pathology Laboratory Parel, Mumbai for chemiluminescence immunoassay to study the levels of testosterone in male Sprague-Dawley rats. The histological analysis was also carried out to confirm the study. From the study it was found that the body weights are inversely proportional to the weight of the testis. It was also found that Ethionamide administered rats showed lowest levels of testosterone, which may lead to infertility. To increase fertility potential we have administered Piper nigrum (Linn.) (PnS) seed ethanolic extract in combination with Ethionamide (ETH) + Para aminosalicylic acid (PAS) drugs. From the above results it is found that Piper nigrum increases the level of testosterone by maintaining the fertility potential in male Sprague-Dawley rats, it is also confirmed that the histological reconciliation of testis was appreciably restored in Sprague-Dawley rats administered with Piper...
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Posted by admin on Oct 31, 2019 in |
Nigella sativa is considered a miracle herb plant belongs to the Ranunculaceae family, inhabitant to South Asia and South-west Asia. The most important part of the Nigella sativa plant used by human beings are seeds, mostly as a spice because it contains a specific aroma. The seeds derived from Nigella sativa being exploited by people and used for thousands of years for culinary purposes, to cure various digestive disorders, recover from joint pains, obesity, common cold, headache, asthma, rheumatic diseases, etc. in India, Pakistan, Bangladesh, China, and Middle East countries. In the current study, the seed extracts of Nigella sativa were targeted for evaluation of qualitative biochemical analysis of phytochemicals, antibacterial, cytotoxicity, and antioxidant properties. Nanoparticles formulations derived as of the aqueous extract of seeds of Nigella sativa were developed and tested against selected microorganisms. FTIR and HPLC also performed to evaluate the functional group analysis and to detect the presence of a bioactive compound in the phytochemical extract. Cytotoxicity properties of methanolic and ethanolic seed extracts of Nigella...
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