Posted by admin on Oct 31, 2019 in |
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the simultaneous estimation of cobicistat and elvitegravir in the pharmaceutical dosage form. A column of ODS (250mm 4.6mm; i.d and 5µ particle size) was used along with the mobile phase comprising of 0.02M dipotassium hydrogen orthophosphate buffer (pH adjusted to 3.3) and methanol in the ratio of 80:20 (v/v). The flow rate was maintained at 1.0 ml/min and the effluents monitored at 254 nm. The retention time for cobicistat was found to be 2.58 ± 0.3 min and elvitegravir was 3.71 ± 0.3 min. The detection concentration was linear over 125-750 µg/ml for cobicistat and 12.5-75 µg/ml for elvitegravir. Regression equations of cobicistat and elvitegravir were found to be y = 25883x + 19711 and y = 27696x + 6046 respectively with regression co-efficient 0.999. The % RSD for Intra and Inter day precision was < 2%. The accuracy of method was validated by recovery studies and found to be significant within acceptable range 98-102%. The developed...
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Posted by admin on Oct 31, 2019 in |
Metal (II) complexes of Co, Ni and Cu with Schiff base derived from 2, 4-Dihydroxy-5-acetylacetophenone and Aminothiophenol (DAAAP) were synthesized and characterized by elemental analysis, FTIR, IH NMR, electronic spectra, thermal analysis, magnetic susceptibility and conductometric measurements. On the basis of these studies, a six coordinated octahedral geometry has been proposed for all the complexes. The Schiff base and its complexes were tested for biological activity against various types of Gram-positive (Bacillus subtilis ATTC 6051 and S. pyogones ATTC 12600) and Gram – negative bacteria (Escherichia coli ATTC 11775 and Proteus vulgaris ATTC 13315) and Fusarium solani Martius and Aspergillus niger Fungus. It has been found that metal complexes are found to have sensitivity for inhibition of Gram‐positive more than Gram‐negative bacteria. All the metal complexes show antifungal activity and it is clear from the data that the complexes exhibit more antifungal activity compared to the parent...
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Posted by admin on Oct 31, 2019 in |
Objective: To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were extracted with methanol. Methanolic extract prepared separated with n-butanol, chloroform, and ethyl acetate as total saponin, total alkaloid and phenolic compound fractions respectively. The chemical characterization of oleanolic acid was done by High-Performance Thin Layer Chromatography (HPTLC) in a mixture of toluene: methanol (9:1) as a mobile phase which developed in anisaldehyde sulphuric acid reagent. Result: The extraction yield of total saponin fraction was 4.3% (w/w). The Rf value 0.31 found to same as the value of standard oleanolic acid. Linear regression analysis data for the calibration plots showed a good linear relationship between concentration (1-6 μg) R (r2) = 0.992408 concerning peak height and area. The limit of detection and limit of quantification of Oleanolic acid were detected to be 10.75 μg and 40.70 μg per spot. The amount of oleanolic acid...
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Posted by admin on Oct 31, 2019 in |
Taraxacum officinale (TO) Weber commonly known as dandelion has contained triterepenes-taraxasterol and taraxerol in its roots which are nowadays being recognized as potent anticancer compounds. The main objective behind the present study was to increase the yield of taraxasterol and taraxerol through induction of hairy roots in Taraxacum officinale Weber using two strains of Agrobacterium rhizogenes MTCC- 532 and MTCC-2084. An efficient protocol for the induction of hairy roots is proposed from the root callus cultures of Taraxacum officinale maintained on MS + IAA + BA + 2, 4-D (0.5 ppm + 1 ppm + 0.5 ppm) through infection of the root callus with two strains of Agrobacterium rhizogenes MTCC-532 and MTCC-2084. It was found that MTCC-532 proved to be effective in hairy roots induction whereas MTCC-2084 failed to induce hairy roots. HPLC results showed the retention time of taraxerol and taraxasterol at 4.975 and 3.5 min respectively. Showing fourfold increase in content of taraxasterol and a nearly two-fold increase in...
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Posted by admin on Oct 31, 2019 in |
Dissolution testing with biorelevant media is used in the pharmaceutical industry as a predictive tool for the estimation of drug formulation’s in-vivo performance in bioequivalence studies. The objective of this study was to determine the biorelevant dissolution media enabling the prediction of in-vivo performance of Valsartan and Chlorthalidone in solid oral dosage form as new fixed drug combination. Dissolution profiles were performed in different pH medias for the evaluation of Valsartan/Chlorthalidone film-coated tablets. The validation studies of the used dissolution method were conducted by performing the parameters specified in the ICH (The International Conference on Harmonization) Q2 (R1) Guideline and the results met the acceptance criteria. Although in-vitro dissolution test findings showed the similarity of release profile of test and mono reference drug products, the in-vivo results demonstrated that they are not similar. For this reason, biorelevant media was investigated and defined. This media proved to be biorelevant and has the potential to be further used to establish in-vitro in-vivo correlation (IVIVC) during the development of Valsartan and Chlorthalidone...
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