Posted by admin on Jul 31, 2019 in |
Klebsiella pneumoniae is a significant pathogen for nosocomial infections and quickly becomes the most prevalent beta-lactamases produced that are resistant to several antimicrobials. To comprehend deeper the condition of multidrug resistance [MDR]. Objectives: Our research was directed at identifying Klebsiella pneumoniae isolates containing shv β-lactamase gene and the relationship between them and some antimicrobial resistance in order to prevent rejection of therapy. Methods: Overall, 64 K. pneumonia it gathered pneumonia isolates and conducted a drug susceptibility test for 13 different antibiotics by the system Vitek 2 to identify their susceptibility patterns. Phenotypic tests for species producing ß-lactamase have determined the resistance phenotype of isolates. Results: The information indicated that 60[93.7%] of the entire isolates tested were Shv genes, while the remainder were positive. The PCR was used to amplified drug resistance. Highest antibiotic resistance in instances of ESBL positive. In fact, 2[33.3%] of the phenotypical confirmatory Test [PCT] sample were shown to be negative isolates. The data has shown that prediagnosed positive phénotype isolates were genetically positive for EsBL development. Concept: the...
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Posted by admin on Jul 31, 2019 in |
Tectaria macrodonta C. Chr. locally known as Kaali negro in Sikkim is a rhizome and spore-bearing terrestrial fern, traditionally used in folk medicine. In the present study, the aqueous, ethanol and methanol extracts of the rhizomes of Tectaria macrodonta C. Chr were evaluated for its potential antibacterial and antioxidant properties. The phytochemical analyses of different solvent extracts were done. Antibacterial activity was determined by the agar well diffusion method against some gram-positive and gram-negative bacteria. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the plant extracts against the test bacteria were determined using the broth dilution method. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, ferric reducing power assay (FRAP) and hydroxyl radical (OH) scavenging assay. Potential antibacterial and antioxidant activity were exhibited by the methanol and ethanol extracts of Tectaria macrodonta. The methanol extract was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The results of the present study suggest the potential pharmaceutical importance of Tectaria macrodonta C. Chr. concerning its antibacterial...
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Posted by admin on Jul 31, 2019 in |
Paclitaxel is one of the best anti-cancer drugs and recently known as the best anti-cancer products of natural origin. The purpose of the present work was to study the physicochemical properties and compatibility of paclitaxel with the selected surfactants employed in nanocrystal formulations. The melting point and loss on drying of paclitaxel were found 216 °C and 0.11% respectively. A log P value of paclitaxel in different solvent systems like Octanol-Water, Dichloromethane-Water, Hexane-Water, and Oleyl Alcohol-Water was found 2.66, 2.91, 3.02 and 3.42 respectively. Paclitaxel is insoluble in water, soluble in ethanol, dichloromethane, and DMSO (dimethyl sulphoxide). The compatibility analysis of Paclitaxel with Egg lecithin, Poly Vinyl Pyrrolidine (PVP) and Poly (L‐lactic acid‐co‐glycolic acid) (PLGA) was studied by employing Fourier transform infrared spectroscopy (FTIR), X-Ray Powder Diffraction (XRPD) and Differential Scanning Calorimetry (DSC) techniques. FTIR, XRPD and DSC methods applied to the mentioned physical mixtures did not show evidence of interactions in the solid state. Based on these results supplied by FTIR, DSC, and XRPD, all the surfactants were...
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Posted by admin on Jul 31, 2019 in |
The present study was designed to investigate the antioxidant and immunomodulatory activity of Azathioprine-Pioglitazone combination therapy to check whether it has added on benefit over monotherapy with Azathioprine or Pioglitazone in rodent models. The test drugs in combination showed better inhibition of free radicals in both H2O2 radical scavenging assay and nitric oxide scavenging assay than individual counterparts revealing its potential antioxidant activity. The combination showed its significant effect on both cell-mediated and humoral immunity to suppress stimulated immune responses in delayed-type hypersensitivity test, carbon clearance test, and nitro blue tetrazolium test. The study proved that Pioglitazone enhances the antioxidant and immunomodulatory activity of Azathioprine indicating the possible synergistic interaction between...
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Posted by admin on Jul 31, 2019 in |
In the present study the 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one isolated from the methanolic extract of leaves of Andrographis echioides was studied for alpha-amylase and alpha-glucosidase inhibition using an in-vitro model. The isolation was done using column chromatography using gradient elution with different mobile phase. Structural elucidation was carried out on the basis of spectral analysis. The study revealed that the 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 36.2 ± 0.42 and 41.4 ± 0.34% respectively and well compared with standard acarbose drug. The infra-red spectrum specific absorption bands for 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one viz. 3401.71 (O-H stretching vibration of phenol), 1666.95 (C=O Aryl ketonic stretch), 1612.42 (C-C Aromatic ring stretch), 1519.96 (C=O aromatic stretch), 1463.09 (C=C aromatic stretch), 1380.89 (O-H bending of phenols), 1318.98 (C-H bond in Aromatic hydrocarbon), 1246.04 (C-O stretch of Aryl ether), 1213.26 (C-O stretch of phenol), 1169.06 (C-CO-C stretch and bending in ketone), 932.62, 807.89, 702.92, 638.65 (C-H bending of aromatic hydrocarbons). Structural elucidation of 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one was done by spectrum analysis such as 13C and...
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