Posted by admin on Oct 31, 2019 in |
Objective: To investigate the anti-proliferative effects of T. procumbens leaf extracts on the growth of MCF 7, Hep G2 and K562 cells. An attempt was made to partially purify the cytotoxic acetone extract using a bioassay-guided isolation procedure and the apoptosis-inducing activity of bioactive sub-fraction, F15 (fraction 15), was probed further against K562 cells. Methods: Cytotoxicity of extracts was evaluated by MTT assay. Hoechst 33258, acridine orange/ethidium bromide and AnnexinV- FITC staining was performed to detect apoptosis. Mitochondrial membrane potential and cell cycle distribution were measured by flow cytometry. DNA fragmentation, alkaline comet assays and Western blotting of key apoptosis-related proteins were also performed. Results: The F15 showed the highest antiproliferative activity against K562 cells leaving the normal peripheral blood lymphocyte unaffected. TLC of F15 displayed a total number of four bands that individually exhibited lower anticancer activities than that of F15, suggesting the possibility of synergism among them. F15 induced phosphatidylserine externalization, loss of mitochondrial membrane depolarization, DNA laddering, increased sub-G1 population and altered expression of caspase 3,...
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Posted by admin on Oct 31, 2019 in |
Industrial soil contains a variety of L-Asparaginase producing microorganisms such as bacteria and fungi. Microorganisms can produce different types of enzymes which are frequently used in different types of industries for different purpose. In the present study, we focused on isolation of L-Asparaginase enzyme producing bacteria from industrial soil samples collected from different regions of Gwalior. These bacterial isolates were Salmonella sp., Proteus vulgaris, Bacillus subtilis which were identified based on colony morphology, Gram staining, biochemical tests and using selective and differential media. All the tested isolates were able to produce enzymes like L-Asparaginase. All the three bacteria which were isolated from soils of a different region of Gwalior able to produce L-Asparaginase enzyme. Maximum L-Asparaginase activity was shown in second (II) bacterial isolate Salmonella sp., which was 12.734 mg/ml. Salmonella sp. were not reported to show any type of allergenicity when their sequences were checked by bioinformatic tool Algpred. So, these bacterial isolates are considered as a good source of enzyme production and may be used at an...
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Posted by admin on Oct 31, 2019 in |
The study was aimed to discover the properties of Trigonella foenum-graecum L. seed mucilage (TFGSM) in the formulation of mucoadhesive microspheres of diclofenac sodium to prolong the residence time at the site of absorption. Diclofenac sodium microspheres were formulated by ion gelation technique. Different polymer ratios of TFGSM and sodium alginate were used to formulate eight formulations (F1 to F8) of TFGSM-based mucoadhesive microspheres of diclofenac sodium and characterized by determining their percentage yield, drug entrapment efficiency, swelling index, mucoadhesive and drug release. The drug entrapment efficiency of all eight formulations was within the range of 78 to 98% with the sustained in-vitro release of over 8 h. The in-vitro drug release of these microspheres followed controlled release (zero-order). The microspheres possessed good swelling properties and mucoadhesive properties. Diclofenac sodium microspheres were observed to adhere strongly with gastric mucosa with approximately 14 h of prolonged stay expecting improved bioavailability and reduced dosing frequency and subsequently improving patient’s compliance. Diclofenac sodium microspheres can be effectively used for sustained drug release...
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Posted by admin on Oct 31, 2019 in |
The aim of the present research was to develop and optimize novel expandable gastro retentive formulation of metronidazole to give local action for the management of Helicobacter pylori infection. The formulation contained a drug-loaded sandwich patch prepared by solvent casting method folded into a hard gelatin capsule. Metronidazole being needle shape crystals soluble in 0.1 N HCl, but after drying, the formulation show bursting effect in first 1 h. To avoid this drug-loaded middle layer containing Xanthan gum and HPMC E15 as release retardants was sandwiched between baking layers of HPMC E15. Drug loaded middle layer was subjected to 32 full factorial design with concentration of HPMC E15 (X1) and Xanthan gum (X2) as independent variables and release of the metronidazole at 1st h and at 4th h as Responses. According to 32 full factorial design 9 batches were prepared and evaluated for thickness, folding endurance, mucoadhesion, drug content and % drug release. The thickness of formulations F1 to F6 was found to be in the range of 0.266...
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Posted by admin on Oct 31, 2019 in |
A simple new rapid, accurate, robust, and specific HPLC method was developed for the assay of pantoprazole and domperidone from the oral solid dosage pharmaceutical formulations. The reverse-phase chromatographic method was developed on an RP C8 column (250 mm × 4.6 mm, 5 µm) using a mixture of 25 mM sodium dihydrogen phosphate solution of pH 6.8 and methanol in the ratio 40:60 v/v as mobile phase in an isocratic mode of elution at a flow rate of 1.0 ml/min at 35 ºC with a load of 20 µl. The detection was carried out at 286 nm. The method was validated concerning linearity, robustness, precision, accuracy, specificity & stability as per ICH guidelines. The method produced excellent separation with good linear correlation coefficients (≥ 0.999) for both the components. The proposed method could be successfully applied for the assay of pantoprazole and domperidone in the various oral solid dosages pharmaceutical formulations, namely tablets and capsules in the sustained release...
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