Posted by admin on Jul 31, 2019 in |
The purpose of the present study was to explore the effect of co-administration of bioenhancer quercetin on membrane permeability of poorly permeable berberine chloride, on goat intestinal membrane model. The effect of co-administration of quercetin was investigated at 2, 6, and 10 mg concentrations. The study revealed a beneficial effect of low concentration of quercetin on % cumulative drug release (% CDR) of the drug under treatment. The co-administration process resulted in remarkable improvement in the permeability of berberine chloride (% CDR 28.33 ± 1.87) at 2 mg of quercetin. On the contrary, the permeability of berberine chloride was decreased (% CDR 10.46 ± 1.55) at 10 mg concentration of quercetin as compared to berberine chloride alone (% CDR 8.49 ± 1.45 at 10 mg). Apparent permeability coefficient, flux, and enhancement ratio were also found to be increased significantly with decreasing concentration of quercetin as compared with the control. It could be concluded that the use of quercetin will be beneficial for co-administration to enhance the permeability, bioavailability, and...
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Posted by admin on Jul 31, 2019 in |
Introduction: Antioxidant properties of flavonoids may guard against oxidative damage to cells, DNA, or lipids. The ample literature about flavonoids can affirm the structural and pharmacological cherishing of this class of phytochemicals. The presence of aromatic rings in the flavonoid molecule consent the receiving and donation of electrons from free radical species, which abets to curb the free radicals. Methodology: This is a prospective observational study conducted on diabetic patient’s over-period of one year in the outpatient department of a tertiary care teaching Hospital. Red blood cells (RBC) were separated from the 20 ml of blood samples, which have been collected from the 90 subjects. The collected RBC were divided into four equal parts for which superoxide dismutase, catalase, and glutathione peroxidase activity was estimated with and without the flavonoids. The absorbance was recorded in a spectrophotometer. Results and Discussion: The results symbolize a significantly higher superoxide dismutase, catalase, and glutathione peroxidase activities for quercetin than kaempferol and chrysin. Quercetin is more effective on Superoxide dismutase followed by kaempferol and chrysin. But there...
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Posted by admin on Jul 31, 2019 in |
A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of Aspirin (ASP) and Omeprazole (OMP). By using box benchen design method have been developed and optimized. Effective chromatographic separation achieved using C18 column (250 × 4.6 mm, 5 µm) as a stationary phase and mobile phase consisted of methanol: Disodium hydrogen phosphate buffer (68: 32 v/v), pH adjusted to 4.5 with phosphoric acid at a flow rate 1.15 mL/min at a detection wavelength of 280 nm. Analytical Quality by Design approach was applied to evaluate the effect of three factors are the volume of the organic phase in the mobile phase, pH of mobile phase and flow rate on chromatographic responses like retention time and tailing factor. The retention time of ASP and OMP were found to be 2.94 and 5.87 min respectively. Calibration curves were found to be linear over the concentration range of 10-60 µg/mL for ASP and 5-30 µg/mL for OMP. The % recovery of...
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Posted by admin on Jul 31, 2019 in |
Introduction: Type 2 diabetes, which is the most common type, is often a result of excess body weight and physical inactivity in genetically predisposed individuals. Over due course of time diabetes can cause blindness, kidney damage, neurological problems, and coronary artery disease. Improvement in glycemic control is important factors in delaying the onset and progression of diabetes-related complications. In spite of the availability of newer antidiabetic drugs, prevention of complication is still a challenge. Nishamalaki, Patoladi Yoga and Shobhanjan are the Ayurvedic formulations claimed to be effective in preventing nephrotoxicity. Objective: To evaluate the therapeutic efficacy of Nishamalaki, Patoladi Yoga, and Shobhanjan in diabetic nephropathy. Materials and Methods: Total of 36 Wistar rats were used for the study. Diabetes was induced in 30 Wistar rats of either sex with STZ (35 mg/kg) i.p followed by High Fat High Fructose diet (HFHF), (coconut oil+ vanaspati) till the development of Diabetic nephropathy (10wks) which was confirmed by Sr. creatinine, blood urea levels. Out of six groups included in the study, group...
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Posted by admin on Jul 31, 2019 in |
This work aimed to evaluate the quality of some commercial peppermint oil products available in the Egyptian market. Also, analysis of peppermint oil extracted from Mentha piperita L. leaves marketed in Egypt. Thus, quality control of both herb extracted and purchased oil products. Essential oils were analyzed using GC/MS, and the main ingredients of each peppermint oil sample were quantified. The antioxidant potential for each of the tested six oil samples was performed using the DPPH radical scavenging assay using ascorbic acid as a reference standard. Also, their EC50% values were calculated. The quality of the samples had been evaluated according to the European Pharmacopeia limits for each of the major peppermint oil components. Thus, menthol, menthone, menthyl acetate, carvone, and pulegone percent composition in each sample was used to evaluate its quality. All the tested samples were found to possess strong to moderate antioxidant activities with EC50% values ranged from 20.44 and 66.10 depending on the oil chemical composition variation. Percent composition of the major peppermint oil components...
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