Posted by admin on Aug 31, 2024 in |
Osteoarthritis in the hip creates a major cause of pain and functional limitation. Acupuncture is an effective treatment for reducing inflammation and protecting muscles and produce pain control associated with disorder. Acupuncture is a proven, comprehensive method that helps to restore normal body function. The aim of this study is to find out the effect of acupuncture for relieving chronic hip pain. Here we report a 45-year-old female presenting with swelling, redness and pain on the right hip and with left hip congenital dislocation and eventually diagnosed as right hip osteoarthritis. X-ray showed superior congenital dislocation of left hip. The patient was treated with the selected acupoints ST44, ST36, UB 32, UB 35, UB 40, GB 32, LI4, LI11, DU20, P6, UB60, UB11, and GB34 for half an hour in alternate day for one week and then 15 days once followed for 6 months. VAS was used to measure pain. After treatment, the patient showed improvement in pain and functional ability. The patient is under regular check...
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Posted by admin on Aug 31, 2024 in |
Cytochrome P450 2D6 (CYP2D6) is an essential enzyme that affects the safety and effectiveness of a broad range of medications through its metabolism. We provide here an extensive in-silico investigation of new drug candidates that target CYP2D6. To find putative CYP2D6 inhibitors, we used virtual screening, structure-based drug design, molecular docking, and simulation approaches in the Biovia Discovery Studio 2022 framework. By maintaining ADMET and TOPKAT filters, a broad variety of small molecules were subjected to virtual screening. After that, the two chosen compounds, cmp1 and cmp13, were used in molecular docking studies against the 4WNU and 4XRZ proteins to evaluate binding affinities and interactions. For 2815 compounds, ADMET and TOPKAT profiling were done to get non-toxic molecules. The non-toxic molecules after filtrations were taken for the docking studies followed by molecular dynamic simulation of the best complex. The best two molecules after ADMET and TOPKAT profiling were taken for docking studies, where these results demonstrated several intriguing therapeutic options with high binding affinities and favorable interactions of compound cmp1...
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Posted by admin on Aug 31, 2024 in |
Oyster mushrooms are considered functional foods due to their structure. Our work focused on assessing the antioxidant power and determining the secondary metabolite content of mushroom teas formulated from the best-known, cultivated and eaten species. We carried out an ethnonutritional survey, and the species selected were used for the rest. We implemented a mixing plan after which 13 formulations were selected to prepare the various herbal teas. Pleurotus pulmonarius, Pleurotus ostreatus and Pleurotus floridanus were the most cultivated, known, and consumed species. DPPH test revealed that T5, T6 and T2 exhibited the best CI50: 17.39 ± 0.35b, 17.65 ± 1.20b, and 18.93 ± 0.31bc respectively compared to the standard which had a CI50 of: 3.09 ± 1.56a. In the FRAP test, T3, T9 and T11 displayed the best IC50 values: 7.72 ± 2.51b, 10.16 ± 0.14c, 11.00 ± 0.17cd, respectively, while the standard had an IC50 of 1.80 ± 1.06a. In the hydrogen peroxide test, T1, T3 and T4 had the best CI50 values: 23.44 ± 2.94b, 24.98 ±...
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Posted by admin on Aug 31, 2024 in |
Methotrexate (Mtx) is used to treat a number of conditions, including certain types of malignancy. Therefore, the purpose of this study was to investigate the potential protective impact of flavonoid quercetin on toxicity caused by methotrexate using hematological, biochemical, histopathological and oxidative stress approaches. There were four different groups, each group consisting of ten rats. The group 1 was a control, and animals were treated with 0.5 ml of carboxymethyl cellulose. Group 2 and 3 were treated with methotrexate (0.250 mg/kg); quercetin (50 mg/kg) and group 4 were treated with methotrexate (0.250 mg/kg) + quercetin (50 mg/kg). All drugs were given orally via oral gavage once daily for 28 days. At end of the trial, blood samples were collected from all of the animals for hematological, biochemical and oxidative stress parameters estimation. Following the animal’s sacrifice, organs were collected for histopathological examination. The levels of TP, ALB, renal indicators like BUN and creatinine and liver enzymes like AST, ALT, and ALP were markedly elevated by methotrexate at dose rate...
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Posted by admin on Aug 31, 2024 in |
The objective of this research was to design and validate an alternative accurate method for quantitative determination of Amphotericin B. Amphotericin B deoxycholate life-saving drug belongs to the polyene class approved for the treatment of progressive and potentially fatal fungal infections. Amphotericin B was estimated at 382 nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were less than 2% for the routine analysis of drug. The limit of detection and limit of quantitation were 0.5μg/ml and 1.5μg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of Amphotericin B without the interference of common...
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