Posted by admin on Jun 30, 2019 in |
In the present investigation, the chalcone intermediates are synthesized by Claisen-Schmidt condensation reaction between acetanilide and appropriate aromatic aldehydes. Further, these chalcone intermediates were cyclized with phenylhydrazine in glacial acetic acid to give new pyrazolines derivatives using ultrasonic irradiation with higher yields in lesser time. All the synthesized derivatives are characterized by their TLC, Physical constant, FTIR, and 1H NMR. The Chalcone intermediates were screened for their antimicrobial activity while cyclized pyrazolines derivatives were screened in search of potential anti-inflammatory compounds. The compound 4c had shown good potential for anti-inflammatory...
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Posted by admin on Jun 30, 2019 in |
The NSAIDs are popular in reducing acute and chronic inflammation as they have no abuse liability. QSAR (Quantitative structure-activity relationship) approach is a very useful and widespread technique for drug design. 2D QSAR models are based on descriptors derived from a two-dimensional graph representation of a molecule. The 2D QSAR study was performed on selected twenty-four compounds from synthesized indole derivatives for elucidating the structural requirements for COX-2 inhibition using multiple linear regression method. Statistically, significant models were generated using VLife Molecular Design Suite 3.5 software. The physicochemical parameters contributed significantly to biological activity. Amongst all the models generated, model 3 was found to be best with high r2 (squared correlation coefficient) of 0.9382. Model is robust as q2 (cross-validated squared correlation coefficient) value is also high as 0.8557 with good predictive power as indicated by pred_r2 = 0.7443. The model showed two alignment independent (AI) descriptors T_2_O_0 and T_2_N_7 as well as two physicochemical descriptors –ve Potential Surface Area and SA Most Hydrophobic contributing for activity. The present...
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Posted by admin on Jun 30, 2019 in |
The entire plant (Monochoria vaginalis (Burm. f.) C. Presl.) except root is being used as a vegetable and has been used in traditional Indian system of medication for treating various diseases. But there are not many scientific data on the antioxidant and anticancer activity of M. vaginalis. Therefore, we aimed to determine the in-vitro antioxidant and anticancer activity of methanolic extract of M. vaginalis leaves. Standard biochemical methods evaluated the phytochemical investigation. In-vitro antioxidant activities of the methanolic extract of leaves were determined against DPPH, hydroxyl radical, reducing power assay, metal chelating activity, and hydrogen peroxide radicals. Also, we evaluated the anticancer activity of methanolic extract on Hep2 and HeLa lines by MTT calorimetric assay. Phytochemical analysis established the presence of rich secondary metabolites of total phenol (52.66 GAE mg/g), and flavonoids (305.88 QE mg/g). The methanolic extract of M. vaginalis exhibited strong antioxidant property (with IC50 value) against DPPH (52.24 mg/ml), hydroxyl radical (76.08 mg/ml), reducing power (58.15 mg/ml), metal chelating activity (35.70 mg/ml), and hydrogen peroxide scavenging...
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Posted by admin on Jun 30, 2019 in |
Arunachal Pradesh (AP), the North-eastern state of India, is a natural habitat of 550 species of orchids including 37 species of medicinal importance. Though these plants are used extensively by locals for various ailments, very few scientific investigations have been conducted to establish their pharmacological potential. In the present study, we selected Tropidia curculioides, a relatively unexplored orchid to evaluate its cytotoxic potential. As any investigation has not been conducted to evaluate the cytotoxic potential of the extracts or compounds, we performed screening of the compounds, previously isolated by our group for cytotoxic activity against three cancer cells viz., MCF-7, A549, and SiHa cells. Interestingly, all three compounds exhibited IC50 in the range of 30-50 µM. Further, we studied apoptosis inducing property and ROS mediated cell death with the most active compound. The result indicated that TcR-3, a gallic acid derivative possesses maximum activity with IC50 of 34.26 µM against A549 cells. The cell proliferation assay and apoptosis-inducing property measured by flow cytometry indicated that the compound has comparable...
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Posted by admin on Jun 30, 2019 in |
Syzygium cumini L. belongs to the family Myrtaceae is one of the widely used medicinal plants in the treatment of various diseases particularly diabetes. The present study has been focused on the synthesis of silver nanoparticles of leaf, seed and fruit extracts of S. cumini and to observe their antimicrobial activity. The synthesized nanoparticles were characterized by performing UV-Vis spectrophotometer, SEM and TEM. UV visible spectroscopic analysis revealed the Surface Plasmon Resonance (SPR), the final reaction product confirming the reduction of AgNO3 to silver nanoparticles. Spherical, triangular and cubical images are observed in SEM analysis of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. TEM analysis also revealed the formation of 5 to 20 sizes of silver nanoparticles with the presence of the layer around the particle. Subsequently tested antimicrobial activity of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. Nanoparticles from seed extracts showed more antibacterial activity on Pseudomonas, followed by Bacillus, Klebsiella, Staphylococci and E. coli. Seed extracts also...
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