Posted by admin on Aug 31, 2019 in |
The plant Knema attenuata or Myristica attenuata (Myristicaceae) is an endemic tree species found in the Western Ghats. Traditionally, the stem bark of K. attenuata is used in folk medicine without any scientific information available for the same. Phytochemical screening was carried out for ethanolic stem bark extract of K. attenuata to investigate the antioxidant potential of the extract. The antioxidant activity of the extract was estimated employing in-vitro models: DPPH radical scavenging assay, total antioxidant activity, nitric oxide scavenging assay and reducing power activity. The extract was found to be rich in phenolics, alkaloids, and flavonoids. Quantitative estimation of extract demonstrated higher levels of phenolics and flavonoids. The extract exhibited significant radical scavenging activity and a total antioxidant capacity compared with that of ascorbic acid (Standard) which confirms the use of the species in folk...
Read More
Posted by admin on Aug 31, 2019 in |
Objective: Studies have shown that sorafenib an anti-cancer agent has a neuroprotective effect. This study evaluated the neuroprotective activity of sorafenib in 6-OHDA induced rat model of Parkinson’s disease. Methods: 6-OHDA was injected into the forebrain bundle through the stereotaxic apparatus to induce fast and severe degeneration in dopaminergic neurons of substantianigra. The animals were divided into four groups. Group I- vehicle control, Group II- 6-OHDA induced, Group III- 6- OHDA + Levodopa (6 mg/kg), Group IV- 6-OHDA + sorafenib (10 mg/kg s.c). Treatment was given for 21 days after induction of 6-OHDA. The animals were subjected to behavioral parameters such as apomorphine-induced rotations, grip strength, catatonia and biochemical parameters such as total protein estimation, reduced glutathione, lipid peroxidase, calcium concentration in the brain. Results: Sorafenib significantly decreased the apomorphine-induced rotations as well as catatonia and significantly increased (p<0.001) the grip strength when compared to 6-OHDA. In biochemical estimation total protein and glutathione is increased (p<0.001). Both lipid peroxidase and calcium level have been decreased significantly (p<0.001) when compared...
Read More
Posted by admin on Aug 31, 2019 in |
Ethno-medicinal plants have a significant role in the field of natural product research and drug discovery since ancient times. Drymaria cordata (L.) Willd. Ex Schult. belongs to the family Caryophyllaceae is an ethnomedicinal plant used as a home remedy in jaundice by the tea tribes of erstwhile Nagaon district of Assam. The present study deals with the evaluation of the phytoconstituents of Drymaria cordata using methanol extract. All the standard phytochemical procedures were followed for the detection of the phytoconstituents. The preliminary phytochemical screening revealed the occurrence of alkaloids, flavonoids, phenols, tannins and saponins. The presence of O-H stretch, C-O stretch, C-H stretch, C-H bend, N-H stretch and N-H bend were confirmed through FT-IR analysis. Several bioactive compounds like Cyclohexan-1,4,5-triol-3-one-1-carboxylic acid, Beta-D-glucopyranose-1,6-anhydro, L-gala-L-ido-octose, 3, 7, 11, 15-Tetra-methyl-2-hexadecen-1-ol, n-Hexadecanoic acid, 9, 12 Octadecadienoic acids (Z,Z)-, 9,12-Octadecadienoic acid, methyl ester, Oleyl alcohol and 17-Octadecynoic acid were identified through GC-MS analysis. From this study, it can be concluded that Drymaria cordata possesses several medicinally important secondary metabolites. Further phytochemical and pharmacological investigations...
Read More
Posted by admin on Aug 31, 2019 in |
Amantadine hydrochloride (1), a well-known antiviral drug, has been used to treat certain type-A influenza infections and been also medicated as an anti-dyskinetic agent for Parkinson’s disease. In this paper, we report a simple and economical procedure for synthesis of amantadine hydrochloride (1) from 1-bromoadamantane (3) and urea in a one-step procedure with high yield (81%) and advantage. The structure of the obtained amantadine hydrochloride was confirmed by MS, 1H-NMR and 13C-NMR spectra and its quality was validated following the United State Pharmacopeia (USP 38). In addition, the synthesis condition was optimized following parameters including reaction reagent as source of nitrogen, reaction temperature, reaction time, reagent molar-ratio, solvent and HCl concentration, successively, to archive the highest yield of amantadine hydrochloride (1) at the following certified condition: reaction temperature = 175°C; reaction time = 1.0 h; molar ratio of (urea: 1-bromo-adamantane) = (3 : 1), in diphenyl ether with ratio of (Ad-Br : Ph2O) = 1.1 g :1 mL),...
Read More
Posted by admin on Jul 31, 2019 in |
The objective of this study was to formulate luteolin with radioisotope Technetium-99m (99mTc) to be 99mTc-Luteolin as an antioxidant compound. After optimization, this radiopharmaceutical preparation should be examined for its physicochemical characteristics, such as radiochemical purity, stability, lipophilicity, protein plasma binding, electronic charge, and stability test. The formulation was done by mixing the luteolin compound with radioisotope 99mTc by using SnCl2 as a reducing agent. Radiopharmaceutical compound 99mTc-Luteolin was formed, then tested for physicochemical characteristics. The result of radiochemical purity was 98.19% ± 1.30%, Log P 0.95 ± 0.07, protein plasma binding 38.01% ± 4.28%, and had neutral charges, test stability at room temperature showed that the radiopharmaceutical purity value was better than in plasma. The results obtained in this research work indicated 99mTc-Luteolin radiopharmaceutical compounds had good physico-chemical properties and were relatively stable in room temperature than in blood plasma. This optimization condition has the potential to be developed into a radiopharmaceutical...
Read More
