Posted by admin on Jun 30, 2019 in |
In an attempt to reveal the medicinal values of Orthosiphon glabratus Benth leaf, which claims to have traditional use for the management of diabetes. In-vitro antioxidant and antidiabetic potentials of Orthosiphon glabratus leaf in different solvent fractions were studied by utilizing DPPH, ABTS, and α-amylase, α-glucosidase inhibitory methods respectively. A preliminary phytochemical study shows the presence of important constituents such as alkaloids, flavonoids, phenols, cardiac glycosides and terpenoids in all the solvent fractions. The ethyl acetate fraction exhibits the best inhibition activity against DPPH and ABTS, and its IC50 was found to be 2.28 and 1.68 µg/mL respectively, which is significantly lower (P<0.05) than reference. In case of antidiabetic activity, ethyl acetate fraction shows the highest enzyme inhibitory action against α-amylase and α-glucosidase and IC50 value was found to be 2.09 and 4.66 µg/mL respectively than (P<0.05) acarbose. The results of in-vitro antioxidant and antidiabetic assays on different solvent fractions of Orthosiphon glabratus leaf, it is evident that the ethyl acetate fraction could be potential candidature, which possessing the...
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Posted by admin on Jun 30, 2019 in |
A new precise, selective, accurate and rapid Ultra Performance Liquid Chromatographic stability indicating method has been developed and validated for simultaneous quantitative determination of Dapagliflozin and Saxagliptin in bulk and pharmaceutical dosage form. The chromatographic separation was achieved with HSS 100 × 2.1 mm, 1.8 m size column. The optimized mobile phase consisting of Phosphate buffer: acetonitrile in the ratio of 50:50 v/v. The flow rate was 0.3 mL/min, and eluents were detected at 260 nm using a PDA detector. The retention time of Dapagliflozin and Saxagliptin were found to be 0.900 and 1.119 min respectively. The percentage recoveries for both the molecules were found to be in the range of 98.61 -100.5%. The calibration curve was constructed between peak areas vs. concentration and demonstrated good linearity in the range of (2.5-15 µg/ml Dapagliflozin and 1.25-7.5 µg/ml Saxagliptin). Degradation studies were carried for Dapagliflozin and Saxagliptin under various stress conditions such as acid, base, oxidation, thermal, photochemical and UV degradation peaks were resolved...
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Posted by admin on Jun 30, 2019 in |
Acanthophora Spicifera is a species of marine red seaweed and in the family Rhodomelaceae. They were freshly collected from Mandapam Coastal Area, Rameswaram Tamil Nadu, India and rinsed in seawater and packed in aseptic bags for further proceedings to the laboratory. Seaweeds are potential renewable resources in the marine environment. It has been used as antioxidant and antimutagen. The results of the present study revealed the reduction of cell viability after the treatment of methanolic extract of Acanthophora spicifera (10-200 µg/mL). In DNA Fragmentation by comet assay showed the methanolic extract of Acanthophora spicifera, the presence of the tail reflect the DNA damage and the potent cell mortality was shown at the concentration 200 µg/ml then decreased significantly with the decreased concentration of extract. Our study also demonstrated the revealed the protective effect of Acanthophora spicifera in HT-29 cell line treated with the methanolic extract of Acanthophora spicifera. Therefore, we concluded that Acanthophora spicifera might be used as a rich source of phytoconstituents and natural...
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Posted by admin on Jun 30, 2019 in |
The present study was designed to assess the pharmacokinetics of drug metabolized by hepatic cytochrome 3A4 (CYP3A4) in human volunteers with physical activity at the high altitude hypobaric hypoxic environment. The International Physical Activity Questionnaire short form 7 (IPAQ SF-7) was used to measure the physical activity. The total of 24 healthy male volunteers participated in this study; for them, the intensity of physical activity was measured in terms of metabolic equivalence of tasks (METs). The pharmacokinetic study was conducted using an oral dose of Alprazolam. The blood samples were collected at fixed intervals up to 24 h. The pharmacokinetic variables were found to be changed with increased intensity of physical activity. The metabolism phase was significantly decreased in vigorous and walking physical activity groups. The area under the curve (AUC) and half-life were also decreased. The pharmacokinetics of drug was found to be decreased with increased intensity of vigorous and walking physical activity in humans working at high altitude hypobaric hypoxia environment. It was assumed that oxygenation of...
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Posted by admin on Jun 30, 2019 in |
Adverse drug reactions are considered to be among the leading causes of morbidity and mortality. Approximately 5-25% of hospital admissions are due to ADR’s, and 6-15% of hospitalized patients experience serious adverse drug reactions (SADR’s), causing significant prolongation of hospital stay. The adverse drug reactions reported were classified based on Wills and Brown classification, Causality assessment by the Naranjo’s causality assessment scale, the severity of the adverse drug reactions was assessed using modified Hartwig and Siegel severity assessment Scale. Preventability of the adverse drug reactions was assessed using Schumock and Thornton preventability scale. A total of 108 suspected adverse drug reactions (ADR) were reported during the study period. Highest percentages of ADRs were seen in adults (62.03%) than geriatrics (24.07%) followed by pediatrics (13.89%). The present study concluded that continuous ADR monitoring Programme was found to be useful and beneficial in identifying the percentage of ADR occurrence on an annual basis in the hospital and also helpful to the patient and health care professionals for a better therapeutic outcome....
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