Posted by admin on Jul 31, 2019 in |
Platelet or thrombosis is one of the most important factors in blood clots formation. However, activation of platelet aggregation plays a significant role in homeostasis process, but in excess, it causes different cardiovascular diseases such as myocardial infarction, atherothrombosis disease, and coronary artery disease. To reduce the occurrence of these diseases, anti-platelet agents can be used to prevent blood clots formation. Therefore, this study was conducted to test the antiplatelet activity of crude Fucoidan from the brown seaweed (Sargassum polycystum) on mice. The measurement parameters used included bleeding time, coagulation time and decrease in plasma uptake by the addition of ADP as a platelet aggregation agent. The mice used were divided into 5 groups: normal control group (0.1% Na-CMC suspension), positive control group (clopidogrel 75 mg/kg), and the crude Fucoidan doses of 50 mg, 100 mg, and 200 mg. The results showed that positive control and crude Fucoidan with doses of 50 mg, 100 mg, and 200 mg groups could prolong coagulation time and bleeding time and also increase...
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Posted by admin on Jul 31, 2019 in |
Objective: To evaluate the antimicrobial potential of Nocardiopsis flavescens isolated from the marine environment. Methods: Attempts were made to isolate actinomycete strains from marine coastal regions of Andhra Pradesh, India. The soil samples collected were pre-treated with calcium carbonate, diluted and plated on three different media to isolate actinomycetes. All the isolates were screened for antimicrobial activity by using the Kirby-Bauer disc diffusion method. Among the fifty isolates screened, one potent strain designated as VJMS-18 exhibited high antimicrobial activity. The isolate was identified by using micro-morphological, cultural, biochemical, physiological and molecular approaches. Results: The Potent strain was identified as Nocardiopsis flavescens VJMS-18. It showed good growth on YMD agar amended with 3% NaCl. Growth pattern of the isolate was studied by culturing it in YMD broth. The culture broth was extracted with ethyl acetate and tested for antimicrobial activity. It exhibited high antimicrobial activity against gram positive and gram negative bacteria as well as fungi. Conclusion: The present investigation reveals that Nocardiopsis flavescens VJMS-18 isolated from the coastal environment...
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Posted by admin on Jul 31, 2019 in |
The aim of current study was to compare the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of roots of seven Asparagus species from North-West India. Total phenolic content measured by Folin-Ciocalteu method ranged from 3.85 ± 0.25 (A. racemosus) to 7.74 ± 0.03 (A. falcatus), 3.42 ± 0.14 (A. racemosus) to 7.22 ± 0.12 (A. falcatus) and 1.12 ± 0.13 (A. officinalis) to 3.93 ± 0.54 mg/g of dry weight of root powder (A. retrofractus) in methanolic, ethanolic and aqueous extracts, respectively, expressed as gallic acid equivalents. The total flavonoid content determined by Aluminium chloride method ranged from 0.61 ± 0.03 (A. officinalis) to 1.86 ± 0.0303 (A. densiflorus), 0.39 ± 0.01 (A. adscendens) to 1.63 ± 0.0403 (A. densiflorus) and 0.31 ± 0.00 (A. adscendens) to 1.51 ± 0.02mg/gDW (A. densiflorus) in methanolic, ethanolic and aqueous extracts, respectively expressed as rutin equivalents. The results showed that TPC and TFC values were highest in methanolic extracts followed by ethanolic and lowest in aqueous extracts. The...
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Posted by admin on Jul 31, 2019 in |
The present study aims to formulate colon targeted drug delivery of Metronidazole compression coated tablets by using different ratios of chitosan and pectin. Carbopol 934P coating is given for compression coated tablets which makes them able to release the drug at the pH of the colonic fluid. Core tablets of Metronidazole (400 mg) were prepared by using swellable and pH dependent polymers like chitosan and pectin in which PVP-K30 is used as a binder. Drug release profile was evaluated in simulated gastric fluid, intestinal fluid, and simulated colonic fluid. The results of drug release studies performed according to the USP paddle method by using 0.1N HCl for 2 h, pH 7.4 phosphate buffer for 3 h and pH 6.8 phosphate buffer up to 24 h without using rat caecal content. Compression coated tablets containing chitosan as polymer released 95-99% of Metronidazole in a simulated colonic fluid, whereas tablets containing pectin as polymer released 94-99% of Metronidazole. The stability study for prepared tablets at 40 °C / 75% relative humidity...
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Posted by admin on Jul 31, 2019 in |
The purpose of the present study was to explore the effect of co-administration of bioenhancer quercetin on membrane permeability of poorly permeable berberine chloride, on goat intestinal membrane model. The effect of co-administration of quercetin was investigated at 2, 6, and 10 mg concentrations. The study revealed a beneficial effect of low concentration of quercetin on % cumulative drug release (% CDR) of the drug under treatment. The co-administration process resulted in remarkable improvement in the permeability of berberine chloride (% CDR 28.33 ± 1.87) at 2 mg of quercetin. On the contrary, the permeability of berberine chloride was decreased (% CDR 10.46 ± 1.55) at 10 mg concentration of quercetin as compared to berberine chloride alone (% CDR 8.49 ± 1.45 at 10 mg). Apparent permeability coefficient, flux, and enhancement ratio were also found to be increased significantly with decreasing concentration of quercetin as compared with the control. It could be concluded that the use of quercetin will be beneficial for co-administration to enhance the permeability, bioavailability, and...
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