Posted by admin on Jun 30, 2019 in |
Saffron is derived from the flower of Crocus sativus as a dried stigma. Kashmiri saffron is the most expensive herb and in sky-scraping demand by herbal and pharmaceutical industries due to which the illegal trade is prevailing in the industry to earn instant Profit. Detection of adulteration and comparison of genuine saffron vs. fake saffron is the crucial aspect which needs a scientific solution. Samples were taken Mogra (A), Lacha (B), Iranian (C) and unknown grade of saffron (D) from Pampore and Anantnag region of Kashmir. The DNA from the samples was extracted with the help NucleoSpin® Plant II Kit and were amplified with the help of reported universal primers of matk, rbcL and MatK, and bidirectional Sanger sequencing was performed. Only ITS gene showed positive extraction and amplification results. ITS gene was capable of distinguishing the interspecific as well as intraspecific variations. The blind trial conducted on unknown grade sample revealed the presence of substituent, which turned out to be Safflower, i.e. Carthamus tinctorius as observed through nucleotide...
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Posted by admin on Jun 30, 2019 in |
Expansion of secondary metabolites from marine resources mainly from echinoderms has been a subject of intense research due to massive bioactive nature. Isolation of new secondary metabolites from sea stars has been found to be highly polar. An initial assessment and validation of these compounds are necessary to assess human risk. Zebrafish are used as a part of a diverse phase of the drug discovery development and has proved to be a constructive and cost-effective alternative to various mammalian models. The current study was performed to observe the effect of sterol isolate from Stellaster equestris on Zebrafish (Danio rerio). The selected active crude extract (which showed enhanced cytotoxic activity) was subjected to purification and was characterized by spectroscopic analysis. The absorption frequency of the FTIR and NMR analysis resembled the absorption frequency of a steroidal compound. The analyzed compound was then evaluated for toxicity using Danio rerio using DNA fragmentation assay, Embryotoxicity, Fin regeneration, and Cardiotoxicity assay as the endpoints. The fishes were exposed to 4 different concentrations (50...
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Posted by admin on Jun 30, 2019 in |
To assess the effect of Perillyl alcohol (POH) on the lipid profile of high-fat-diet-induced hyperlipidemia in white male albino Wistar rats, the hyperlipidemia was induced by feeding cholesterol-rich high-fat diet for 45 days in male albino Wistar rats. The decline in the triglycerides, LDL and VLDL was significant at 100 & 200 mg/kg dose of POH compared to Atorvastatin group. The decrease in total cholesterol was significant at 100 and 200 mg/kg doses compared to hyperlipidemic control. The results demonstrate that POH at a dose of 100 and 200 mg/kg body weight lowers the serum triglyceride, LDL, VLDL, total cholesterol and increase HDL level significantly in high-fat-diet-induced hyperlipidemic rats. Though there was no significant effect on the lipid profile at 50 mg/kg dose. Thus, the results of the present study provide scientific validation for the use of Perillyl alcohol (POH) a terpene as an Antihyperlipidemic...
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Posted by admin on Jun 30, 2019 in |
The purpose of the research was to assess the prescribing pattern in geriatrics and to analyze the prescription using World Health Organization (WHO) core prescribing indicators and American Geriatric Society (AGS) Beers criteria 2012 for potentially inappropriate medication use in older adults. This prospective drug utilization study was conducted for a period of 6-months. Patients of either gender, age 65 or above who were admitted in the secondary care hospital. Collected data were analyzed with the WHO core prescribing indicators and American Geriatric Society Beers criteria 2012 for potentially inappropriate medication use in older adults. A total of 200 patients were taken in the study. The majority of the patients were in the age group of 65-70 years (64%) with male predominance (53%). The average number of drugs per encounter was 6.86. Out of 1372 drugs, 1157 drugs (84.32%) were prescribed by generic name. Drugs acting on the cardiovascular system (27.92%) were the most commonly prescribed. In this study, Ranitidine (24.4%) is the most commonly prescribed drug. The study...
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Posted by admin on Jun 30, 2019 in |
Entamoeba histolytic is a protozoan parasite which causes amoebiasis, about 50 million people are affected yearly, and it caused more than 100,000 deaths per year. The study investigates the therapeutic effect of ethanolic and aqueous Tamarindus indica leaves extracts on infected rats with Entamoeba histolytica. Twenty-one Rattus norvegicus rats were infected with Entamoeba histolytica and divided into seven groups (three rats in each group). Groups (1, 2, and 3) were treated with aqueous Tamarindus indica extracts at doses 125, 250, and 500 mg/kg respectively for ten days. Groups (4, 5 and 6) were treated with ethanolic extract of Tamarindus indica at doses 125, 250, and 500 mg/kg respectively for ten days. Group (7) was left untreated as a positive control group. The results showed a significant decrease at p≤0.05 in the numbers of parasite cell from the second day of treatment in all the groups treated with aqueous as well as ethanolic extract. This decrease gradually continued during the days of treatment until it reached zero on the last...
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