Posted by admin on Jul 31, 2019 in |
Background: Cisplatin is active cytotoxic agents in the treatment of cancer and has many adverse side effects, including hepatotoxicity. Honey and royal jelly are natural products and having antioxidants properties. Aim of the study: To investigate the protective role of combined administration of honey and royal jelly against cisplatin-induced changes in biomarkers of oxidative stress in rat liver. Materials and methods: Male Wistar albino rats of approximately the same age and weight were randomly divided into four groups. Group I: rats were controlled and were given 0.9% saline. Group II; rats were injected cisplatin (7 mg/kg/day) intraperitoneally for 15 days. Group III; rats were fed orally honey (500 mg/kg/day) with royal jelly (100 mg/kg/day) for 15 days. Group IV; rats have injected cisplatin (7 mg/kg/day) intraperitoneally and fed orally honey (500 mg/kg/day) with royal jelly (100 mg/kg/day) daily for 15 days. At the end of experimental animals were deprived of food overnight and sacrificed by cervical decapitation for estimation of antioxidant enzyme in liver tissue. Results: Administration of cisplatin...
Read More
Posted by admin on Jul 31, 2019 in |
A simple, specific, accurate reversed-phase high performance liquid chromatographic method was developed for the quantification of Cabozantinib. Although extensive studies on Cabozantinib have been developed for determining Cabozantinib in human place and urine by LC-MS, studies on the pharmaceutically active ingredient and formulation are scarce. The effective separation was achieved through BDS C18 150 × 4.6 mm, 5m using a mobile phase KH2PO4 Buffer and ACN (55:45% v/v). The flow rate of the mobile phase was 1.0 mL/min, and the detection was carried at a wavelength of 210 nm. The retention time of Cabozantinib was 2.932 nm. The correlation coefficient is 0.9998. The developed method was validated in terms of system suitability, specificity, linearity range, precision, accuracy, limits of detection and quantification. Degradation studies were performed on Cabozantinib to indicate the stability property and specificity of the proposed method. The information presented in this study will be useful for industrial application for determining Cabozantinib in active pharmaceutical ingredient and pharmaceutical dosage...
Read More
Posted by admin on Jul 31, 2019 in |
Though possessing a lot of ethnopharmacological use, Mimosa diplotricha did not explore thoroughly for its bioactivity & the phytoconstituents responsible for its bioactivity. The purpose of the current work was to conduct phytochemical screening and antinociceptive activity of methanolic extract and its different fractions of Mimosa diplotricha leaves. Phytochemical screening of methanolic extract revealed the presence of alkaloids, carbohydrates, saponins, glycosides, phytosterols, phenols, flavonoids, proteins and lipids in Mimosa diplotricha leaves. The antinociceptive activity was assessed by using acetic acid induced writhing method and tail immersion method at two different doses using Swiss-albino mice as an animal model. In the mice model the methanolic extract and its n-hexane fraction showed Significant peripheral-antinociceptive activity at a dose of 400 mg/kg body weight with percentage of inhibition of acetic acid-induced writhing 56.25% (P<0.001) and 51.62% (P<0.001) respectively compared to the standard diclofenac sodium (62.50%, P<0.001) group. The antinociceptive effect of methanolic extract and its n-hexane fraction of Mimosa diplotricha at the 400 mg/kg and 200mg/kg dose level was also found to...
Read More
Posted by admin on Jul 31, 2019 in |
A series of new Schiff bases were synthesized by condensation of 3-(5-bromothiophen-2-yl)-1-(4-chlorophenyl)-1H-pyrazole-4-carbaldehyde with different aromatic aldehydes. The 3-(5-bromothiophen-2-yl)-1-(4-chlorophenyl)-1H-pyrazole-4-carbaldehyde was prepared from 1-(1-(5-bromothiophen-2-yl)ethylidene)-2-(4-chlorophenyl)hydrazine by the Vilsmeier Haack reaction. The 1-(1-(5-bromothiophen-2-yl)ethylidene)-2-(4-chlorophenyl) hydrazine was prepared by the condensation reaction of 2-acetyl-5-bromothiophene with 4-chlorophynylhydrazine hydrochloride. The structures of newly synthesized compounds were elucidated by NMR, IR, and Mass spectral – data. Prepared Schiff bases were evaluated for antibacterial activity against four organisms: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae using streptomycin as a standard drug. Agar well-diffusion method was followed to determine the antimicrobial activity. All prepared Schiff bases showed poor to good activity against test organisms. Based on the zone of inhibition results, it is observed that the newly prepared Schiff bases showed better activity against Klebsiella pneumoniae than Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Schiff base 4c showed good activity against Pseudomonas aeruginosa, Schiff base 4l showed good activity against Staphylococcus aureus and all the Schiff bases 4a-4l except 4c showed good activities against Klebsiella...
Read More
Posted by admin on Jul 31, 2019 in |
Objective: The Failure in chemotherapy is mainly because of the resistance of the chemotherapeutic drugs towards the neoplastic cells. The main reason behind this study was to develop a novel combination of 5-Fluorouracil (5-FU) lipid curcumin conjugates for the treatment of cancer. Methods: In this study, the lipid group stearic acid and oleic acid was conjugated with the parent drug moiety 5-Fluorouracil to acquire lipophilicity. The next step involves the conjugation of curcumin with 5-Fluorouracil lipid to form a lipid dual drug conjugate. The characterization of conjugates followed the synthesis part by using FT-IR, DSC, and LCMS. The conjugates hence obtained were further carried for formulation aspect. After the successful completion of the formulation part, in-vitro studies and MTT Assay was performed. Results: The results depict the successful synthesis of the conjugates, and its characterization was successfully done. The conjugates are formulated into Self Nanomulsifying drug delivery system (SNEDDS). The results obtained from the MTT assay also shown a significant cytotoxic effect in both the conjugation. Conclusion: The in-vitro...
Read More
