Posted by admin on Jun 30, 2019 in |
The pharmaceutical market of the Russian Federation receives medicines produced both in compliance with the international standards of good manufacturing practice and those coming from developing countries where manufacturers operate according to their national regulations. Asparagus racemosus is a medicinal plant that is widely used in oriental medicine and is included in the Ayurvedic pharmacopeia. The plant, among others contains steroid saponins, isoflavones, and polysaccharides. The roots of Asparagus racemosus are used as a phytoestrogen in disorders of the hormonal system by women. The plant is also known for its antioxidant, immunostimulating, and anti-inflammatory effects. The Russian State Pharmacopoeia (RSP), XIII edition does not include this plant species. However, medicinal plant raw materials (MPRM) and remedies based on them do enter the Russian pharmaceutical market. Therefore, the main objective of this study is to determine the quality and safety of raw Asparagus racemosus. We determinedsuch parameters of quality and safety as: extractive values i.e. alcohol soluble and water soluble; ash values: total ash, ash insoluble in 10% hydrochloric acid...
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Posted by admin on Jun 30, 2019 in |
Objective: To establish the preliminary phytochemical screening and fingerprint profile of stem bark Extracts of Maytenus emarginata by using high-performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated, and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, server vision CATS-server PH, version 2.5.18072.1 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of flavonoids, glycosides, tannins, alkaloids, terpenoids, phenolic compounds, proteins, reducing sugars, fats, and oils. The fingerprint analysis of petroleum ether extract showed four peaks; chloroform extract showed four peaks and methanolic extract showed six peaks in 10 μl of the sample analyzed. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether, chloroform and methanolic extract of stem bark of Maytenus emarginata can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant...
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Posted by admin on Jun 30, 2019 in |
Objective: The present study explores the anticancer potentially of plant sample based on the evidence from the ethnomedicinal practice of the plant. The main objective was to find out the in-vitro comparative anticancer activity of the various methanolic extract. Method: Plants samples such as Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinea galanga were collected, identified, and authenticated. By using Trypan blue test Human cervical cancer (Hela), cells were counted. The Human cervical cancer (Hela) cells were treated with methanolic extract of Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinia galangal and 20 µL of MTT (5 mg/mL) solution was added to cells per well, and the plate was moved to a CO2 incubator (3-4 h incubation). Measurement was performed using a Spectramax M2 Microplate Reader (Molecular Diagnostic, Inc.) at a wavelength of 570 nm. Results: The Cytotoxicity of studied medicinal plants of Mizoram such as Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinia galanga exhibited cytotoxicity in an increased manner with increase concentration against Hela cells. Their IC50...
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Posted by admin on Jun 30, 2019 in |
Context: Endolichenic fungi are the microorganisms living inside the thalli of the lichen and found to be a new source of bioactive secondary metabolites. This is an underutilized source of bioactive compounds and has not been studied extensively. Genus, Parmotrema is one of the foliose lichens with largely rounded lobes and occurs on rocks of rain forests, sub-montane and montane forests in Sri Lanka. Aims: The endolichenic fungi available in Sri Lanka and the chemistry of their secondary metabolites have not been explored thoroughly. This study aims at exploring novel bioactive compounds available in Penicillium citrinum inhabiting Parmotrema sp. Methods and Material: The lichen, Parmotrema sp., was collected from Hakgala Botanical Garden in Sri Lanka and its fungal strains were isolated and identified by Genomic DNA sequencing. Compounds were first extracted to ethyl acetate and subsequently subjected to bioassay-guided fractionation to isolate the bioactive compounds. The DPPH assay was used to determine the antioxidant activity. The active compound/s was separated by column chromatography, and its structure was elucidated by...
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Posted by admin on May 31, 2019 in |
Gastroenteritis, especially those caused by bacterial pathogens, remains as one of the major health issues in developing countries. Despite the availability of treatment, people are turning their interest in consuming certain food for its therapeutic value given the adverse effects of medications. This includes the consumption of date fruits (Phoenix dactylifera L.). This study aims to determine the antibacterial effect of two methods of extraction, i.e., hot aqueous extract and methanol extract of Ajwa date fruit against selected gram-negative bacteria causing gastroenteritis. The antimicrobial activities were determined quantitatively using well diffusion assay together with the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). Results showed that both hot aqueous and methanol extracts have the potential to suppress the growth of all tested bacteria. The methanol extract had higher inhibitory zones compared to hot aqueous extract, and the zones of inhibition were noted to increase with increase in the extract concentration. S. flexneri was the most sensitive bacteria to both types of extract. The methanol extract was also more...
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