Posted by admin on May 31, 2019 in |
Aspergillus flavus is a famous fungus that produces aflatoxins. Aflatoxins are the most well known toxic and carcinogenic chemical prominent produced in nature. The fungus and aflatoxins are the main concern of the corn consumer. Pangi (Pangium edule Reinw) is a potential native plant to reduce infestation of the fungus A. flavus to prevent fungal contamination of food or feed. The research was undertaken to assess the antifungal activity of the P. edule Reinw extracts of seed against fungus A. flavus. Higher concentration is shown as the best concentration to suppress the growth of the A. flavus both mycelial growth and emerging new colony. The concentration of the 15% effectively and consistently inhibits A. flavus than other treatments. The lower concentration of 0.5%, 0.75%, 1% showed not significantly different over the control. Another higher concentration of the 5% showed that there is no inhibition of the fungus growth. However, the concentration of the 10% indicates also as a good treatment to suppress the fungus growth. Colony appearance after 144...
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Posted by admin on May 31, 2019 in |
A stability indicating method was developed and validated for simultaneous estimation of Elvitegravir, Cobicistat, Emtricitabine, and Tenofovir Disoproxil Fumarate (Tenofovir D F) in solid dosage form using RP-UPLC method. Waters Acquity UPLC system with Column Endoversilo C18 (50 × 2.1 nm, 1.8 μm) having PDA detector at 252 nm wavelength was used. Mobile phase having a mixture of 700 ml of Acetonitrile and 300 ml 0.1% Ortho Phosphoric Acid (OPA) in the ratio 70:30 v/v was used. The flow rate was set to 0.3ml/min. The retention time was obtained at 0.59 min for Elvitegravir, 2.51 min for Cobicistat, 1.48 min for Emtricitabine, and 0.73 min for Tenofovir Disoproxil Fumarate respectively with a total run time of 4 min. The linearity was calculated with correlation coefficients (r2=0.999), which were found to be within limits. The percentage recoveries of Elvitegravir, Cobicistat, Emtricitabine, and Tenofovir Disoproxil Fumarate were within the acceptance criteria. The drugs were found to be stable at forced degradation conditions, and the net degradation was found to be within...
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Posted by admin on May 31, 2019 in |
A simple, Precise, Accurate method was developed for the estimation of Inotuzumab Ozogamicin by RP-HPLC and UV technique. Chromatographic conditions used were stationary phase Discovery c18 150 × 4.6 mm, 5 m. Mobile phase O- phosphoric acid buffer: Acetonitrile in the ratio of 50v:50v and the flow rate were maintained at 1 ml/min, detection wavelength at 240nm, column temperature was set to 30 ºC, and diluents was Acetonitrile: Water (50v:50v), Conditions were finalized as an optimized method. System suitability parameters were studied by five times standard was injected, and results were well under acceptance criteria. Linearity study was carried out between 4.5 µg/ml to 27 µg/ml levels, R2 value was found to be as 0.999. Precision was observed to be less than 2% for both repeatability and intermediate precision. LOD and LOQ are found to be 0.033 µg/ml and 0.099 µg/ml respectively. The explored HPLC method was fast, simple, sensitive, accurate and precise for the estimation of Inotuzumab Ozogamicin in bulk and its dosage form. The compounds were subjected...
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Posted by admin on May 31, 2019 in |
A FTIR method was developed to estimate Artemether and Lumefantrine simultaneously in bulk as well as in formulations. It is based on the measurement of absorption of radiation at the absorption band of ether at 1113.10-1091.77 cm-1 for ART and phenyl substitution band at 893.65-857.08 cm-1 for LUM, because those absorption bands did not occur in excipients present in a pharmaceutical preparation. The proposed method was validated as per ICH guidelines. The calibration curve was obtained for a series of concentration in the range of 17-470 mg for ART and 10-250 mg for LUM, and it was found to be linear. The linear regression equation was y = +84.78-193.1*x for ART and y = +353.75+2129.1*x for LUM with correlation coefficient value 0.999 for ART and LUM which were within the acceptance criteria. The precision was measured regarding repeatability and % RSD was calculated and was found to be 0.831 for intraday and 0.831 for inter-day precision. Recovery was carried out standard addition method at three different levels which are...
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Posted by admin on May 31, 2019 in |
The current research was accomplished to assess the analgesic activity of Argyreia cymosa (A. cymosa) methanolic extracts (MEAC) at 200 and 400 mg/kg. The entire herb was extracted with the methanol (MEAC). Acute oral toxicity of the extract was according to OECD-423 recommendations. Analgesic activity was explored by utilizing hot plate, tail immersion and acetic acid-evoked writhing models. In acute toxicity study, no mortality was observed when each extract was orally administered with 2000 mg/kg. At the doses (200 and 400 mg/kg) methanolic extract of Argyreia cymosa demonstrated considerable and dosed reliant analgesic effect. The conclusions from the present investigation confirmed the folkloric usage of Argyreia cymosa as an analgesic agent. This significant analgesic impact might be because of the presence of steroids, flavonoids, and glycosides within the extract. But, further phytochemical, as well as biological tests, are needed to determine the additional active chemical constituents responsible for the antinociceptive...
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