Posted by admin on Aug 31, 2019 in |
The study aimed to develop a specific, exact, selective, precise and accurate Reversed-Phase Stability Indicating Ultra-fast Liquid Chromatography (RP-UFLC) strategy is created and validated for the determination of glimepiride in the tablet dosage form. The method showed an adequate separation for glimepiride from their degradation products. The optimum separation was achieved by using 250 mm × 4.6 mm C18 column (5 μm) with a mixture of potassium dihydrogen orthophosphate adjusted to pH 4.6 using (orthophosphoric acid) and acetonitrile in the ratio of 75:25 at a flow rate of 1 ml/min. The detection was carried out at 236 nm, and the retention time was found to be 5.9 min. Linearity was observed (correlation coefficient r2 0.9989). % R.S.D was found to be less than 2%. Accuracy of the method found to be in the range of 98.0 to 102.00 (% w/w). The above method was validated concerning system suitability, linearity, precision, the limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery) and robustness according to ICH guidelines. The...
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Posted by admin on Aug 31, 2019 in |
γ-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the vertebrate central nervous system (CNS); it activates the GABAA receptors that play a role in epilepsy. Targeting GABAA receptors through specific enhancement of neuronal inhibition involving GABA can be a better mechanism for identification of novel antiepileptic drugs. In this report, screening of novel isolated homoisoflavanones from bulbs of Ledabouria revoluta against human GABRB3 active site using molecular docking studies. The docking analysis reveals the identification of leads with favorable binding energy and hydrogen bond interactions confirmed the effective modulation of the receptor. Based on the dock score and number of hydrogen bond interactions, compound 3 observed to be the most...
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Posted by admin on Aug 31, 2019 in |
The leaves of Coriandrum sativum commonly known as Cilantro and Petroselinum crispum commonly known as Parsley, family Apiaceae, have several health benefit effects. Most of the people, confused while buying these leaves. In the present study, comparative morphological, microscopic, fluorescence, TLC, and phytochemical parameters, comparative assessment of phenolic and flavonoid contents and in-vitro antioxidant activity of C. sativum and P. crispum leaves were carried out using standard methods. The leaves of these plants are morphologically similar, except other characteristics such as smells, number of lobes and serrate. The microscopic study showed the presence of different types of stomata, epidermis, fibers, and trichrome. Comparative fluorescence, ash content, LOD, and extractive content study were shown the distinct features. The total phenols and flavonoids of the leaves of P. crispum were higher than the leaves of C. sativum, and its antioxidant activity was also stronger than the leaves of C. sativum. The present comparative study reports the first time and can be useful for the identification or authentication of P. crispum and...
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Posted by admin on Aug 31, 2019 in |
The plant Knema attenuata or Myristica attenuata (Myristicaceae) is an endemic tree species found in the Western Ghats. Traditionally, the stem bark of K. attenuata is used in folk medicine without any scientific information available for the same. Phytochemical screening was carried out for ethanolic stem bark extract of K. attenuata to investigate the antioxidant potential of the extract. The antioxidant activity of the extract was estimated employing in-vitro models: DPPH radical scavenging assay, total antioxidant activity, nitric oxide scavenging assay and reducing power activity. The extract was found to be rich in phenolics, alkaloids, and flavonoids. Quantitative estimation of extract demonstrated higher levels of phenolics and flavonoids. The extract exhibited significant radical scavenging activity and a total antioxidant capacity compared with that of ascorbic acid (Standard) which confirms the use of the species in folk...
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Posted by admin on Aug 31, 2019 in |
Objective: Studies have shown that sorafenib an anti-cancer agent has a neuroprotective effect. This study evaluated the neuroprotective activity of sorafenib in 6-OHDA induced rat model of Parkinson’s disease. Methods: 6-OHDA was injected into the forebrain bundle through the stereotaxic apparatus to induce fast and severe degeneration in dopaminergic neurons of substantianigra. The animals were divided into four groups. Group I- vehicle control, Group II- 6-OHDA induced, Group III- 6- OHDA + Levodopa (6 mg/kg), Group IV- 6-OHDA + sorafenib (10 mg/kg s.c). Treatment was given for 21 days after induction of 6-OHDA. The animals were subjected to behavioral parameters such as apomorphine-induced rotations, grip strength, catatonia and biochemical parameters such as total protein estimation, reduced glutathione, lipid peroxidase, calcium concentration in the brain. Results: Sorafenib significantly decreased the apomorphine-induced rotations as well as catatonia and significantly increased (p<0.001) the grip strength when compared to 6-OHDA. In biochemical estimation total protein and glutathione is increased (p<0.001). Both lipid peroxidase and calcium level have been decreased significantly (p<0.001) when compared...
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