Posted by admin on Jun 30, 2019 in |
Background: Hypertension is one of the major risk factors for stroke, heart attack, heart failure, and kidney failure, thereby cause of deaths and disability worldwide. The most predominant type of HTN is essential hypertension (HTN). Unani scholars mentioned clinical manifestations and management of hypertension and have documented it under the context of ‘Imtila.’ The drug Sankhaholi (Evolvulus alsinoides Linn.) is one of the widely prescribed medicines for the management of essential hypertension in Unani medicine. Material and Method: Present clinical study was carried out to evaluate the efficacy and safety of Sankhaholi (Evolvulus alsinoides Linn.) in the management of stage-1 essential hypertension. Newly diagnosed 41 patients of Essential Hypertension (22 patients were in the test group and 19 patients in the control group) were enrolled and their clinical, as well as hematological parameters, were recorded at baseline, 21st day and 42nd day. All the patients in the test group were provided with the test drug 3 g powder of Sankhaholi which is to be taken twice a day for...
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Posted by admin on Jun 30, 2019 in |
Clustered regularly interspaced palindromic repeats are the centerpiece of a robust adaptive bacterial immune system that forms the basis for the CRISPR-Cas9 genetic engineering technology. CRISPR-associated protein-9 (Cas9) is part of this immune system that seeks out, cuts, and degrades viral DNA. By taking advantage of the CRISPR-Cas9 system, a tool has been developed to delete or insert DNA into target genes in bacterial cells or mammalian cells for obtaining cells with desired traits. In this study, we used the CRISPR-Cas9 system for engineering the genome of a non-pathogenic, laboratory strain of bacteria Escherichia coli. We introduced a mutation in the gene of the bacteria which converted its phenotype from sensitivity to the antibiotic streptomycin to resistance to streptomycin; thus allowing the bacteria to grown on streptomycin containing plates. We also investigated different transformation conditions to determine which condition gave the maximum number of transformants, which reflects the efficiency of the CRISPR-Cas9 genome editing...
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Posted by admin on Jun 30, 2019 in |
A simple, accurate, precise, and rapid high-performance thin-layer chromatographic (HPTLC) method for determination of Apixaban (API) in bulk drug and the pharmaceutical formulation was developed and validated. The separation was performed on aluminum plates, pre-coated with silica gel 60F254 as the stationary phase using toluene: methanol (7:3 v/v), as mobile phase. Densitometric measurement of the API was carried out at 290 nm. The retention factor (Rf) was found to be 0.62 ± 0.04. The method was successfully validated as per International Conference on Harmonization (ICH) guidelines Q2 (R1). The calibration curve was linear in range 50-300 ng/band. The limits of detection and quantitation were 0.272 and 0.824 ng/band, respectively. Accuracy and precision of the proposed method were evaluated by recovery studies and intra-day and inter-day precision studies, respectively (standard deviation for precision studies was found to be less than 2). The developed method was used to analyze marketed formulation samples (tablet) of the API. The API was subjected to various stress conditions as per ICH Q1A (R2) guidelines. In...
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Posted by admin on Jun 30, 2019 in |
Tinospora cordifolia is a requisite medicinal plant, has been used for the treatment of various diseases and has been recommended for improving the immune system mentioned in Ayurveda and other systems of medicine as a monoherbal or polyherbal preparation. Nonetheless, Ayurvedic and other ancient literature reported that Tinospora cordifolia growing on neem plant gives better immunomodulatory activity. The traditional and scientific data comprising immunomodulatory potential, pharmacognostic description, therapeutic uses, phytochemical constitution, of Tinospora cordifolia and neem guduchi using the aqueous extract in the form of satwa has well established. The present study evaluates the comparative immunomodulatory activity using satwa of Tinospora cordifolia and neem guduchi using in-vitro models, for understanding the influence of edaphic factors on biologically active compounds. Evaluation of the immuno-modulatory potential of the guduchi satwa and neem guduchi satwa at the dose of 150 and 300 mg/kg body weight was done in rats; assessment of immunomodulatory activity was carried out by assessment of neutrophil adhesion whereas phytochemical profile was established by HPTLC method. Simultaneously, the correlation...
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Posted by admin on Jun 30, 2019 in |
Green tea represents a popular beverage worldwide due to its high polyphenol and antioxidant activity. It is commonly brewed along with vitamin C source which plays a positive role in maintaining its antioxidant potential. Orange juice can serve as an excellent medium for supplementation of green tea for the formulation of nutraceutical nectar by enriching drink with vitamin C and overcoming the astringent taste of tea. Therefore, the present study was planned to develop nutraceutical nectar by blending green tea with orange juice at 60, 50, 40 and 30% levels while orange juice and green tea served as control. The formulations were analyzed for their sensory as well as nutritional properties. Among all the samples, the highest values for ash content, ascorbic acid content, titratable acidity, and TSS were recorded for orange juice. Green tea displayed the highest values for total flavonoid content, antioxidant activity (FRAP, DPPH, TEAC), moisture content and pH compared to other blends. Blending green tea with an increasing concentration of orange juice resulted in a...
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