Posted by admin on May 31, 2019 in |
Eurycomanone from the roots of Eurycoma longifolia Jack (ELJ) has been reported to exhibit anticancer activity. Four of ester eurycomanone derivatives: eurycomanone dibutyrate, eurycomanone monovalerate, eurycomanone dimethoxybenzene, and eurycomanone disuccinate were synthesized for knowing their activities on cancer cell lines T47D, MCF-7, Hela, WIDR and Vero cells. The cancer activity was evaluated by the MTT colorimetric assay method. The results showed that eurycomanone has anticancer activity on T47D, MCF-7, Hela, WIDR cancer cells with IC50 values (1.17 ± 0.09; 3.96 ± 0.02; 2.95 ± 0.08; 1.45 ± 0.01 µg/mL), and no toxic to Vero cells (609.89 ± 29.77 µg/mL). Its derivatives eurycomanone dibutyrate have anticancer activity on T47D, MCF-7, Hela, WIDR cancer cells with IC50 values (25.16 ± 2.25; 21.56 ± 4.55; 29.32 ± 1.25; 149.42 ± 12.50µg/mL), eurycomanone monovalerate (25.59 ± 1.31; 22.48 ± 1.25; 30.14 ± 1.89; 91.88 ± 8.90 µg/mL), eurycomanone dimethoxybenzene ( 102.77 ± 2.56; 38.83 ± 2.55; 66.65 ± 1.90; 51.61 ± 2.37µg/mL), eurycomanone disuccinate (218.94 ± 9.30; 198.87 ± 5.50; 166.67 ± 12.34;...
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Posted by admin on May 31, 2019 in |
‘Natural curing agents for diabetes’ is a matter of world research. Diabetes, the gift of altered life pattern, affected considerably larger proportion of world population. Ancient Indian literature has the treasure of knowledge regarding natural curing agents. The plant Operculina turpethum (Convolvulaceae) is one among them. The plant root is used in many Ayurvedic formulations to treat various diseases including diabetes. In the present study, anti-oxidant and anti-diabetic properties of leaf extract were done to find an alternative for root with an aim to promote sustainable utilization of the plant in future. The methanol extract of the leaf was fractionated with hexane and ethyl acetate, and the residue was dissolved in methanol. The antioxidant and hypoglycemic effect of these fractions were analyzed using standard protocols. The results projected anti-diabetic efficacy of the plant leaf and also powerful anti-diabetic agents in ethyl acetate fraction. The phytochemical quantification of the flavonoid content in the fractions revealed considerably high amount of flavonoids in ethyl acetate fraction than the other two. Based on this preliminary results, it...
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Posted by admin on May 31, 2019 in |
The Phlogacanthus thyrsiflorus roots have been used by the tribal population of western Assam to treat the hepatic disorder. The present study focuses on phytochemical screening, GC-MS analysis and in-vitro antioxidant activities using different extracts. Plant total phenolics, flavonoids, reducing power assay and antioxidant activity was evaluated. DPPH, FRAP, ABTS, Metal chelating activity, and H2O2 free radical scavenging activity were studied. The screening from extracts showed the presence of phenols, flavonoids, tannins, resins, terpenoids, alkaloids, glycosides, cardiac glycosides, steroids. The highest concentration of total phenolics, flavonoids, reducing power and total antioxidant activity was found in the hydro-alcoholic extract of RoPt. The results of the radical scavenging activity in 70% ethanolic extract showed better IC50 value in DPPH (265.87 ± 17.58), ABTS (17.89 ± 1.18) and H2O2 (17.89 ± 1.05) µg/ml respectively. EC50 value of 535.16 ± 34.82 was found in 70% ethanolic against 1471.32 ± 19.77 µg/ml of acetone extract whereas the FRAP showed high FeSO4.7H2O concentration in acetone extract with 820 ± 9.1 µM/ml. The major chemical constituents that were identified in GC-MS analysis were 2R-Acetoxymethyl-1,3,3-Trimethyl-4T-(3-Methyl-2-Buten-1-YL)-1T- Cyclohexanol (71.243%) and 2,4,4-Trimethyl-3-Hydroxymethyl -5A-(3-Methyl-But-2-Enyl)-...
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Posted by admin on May 31, 2019 in |
Biofilm in chronic rhinosinusitis (CRS) is the infection by bacteria, which is difficult to overcome. It has recurrent infections, mucosal inflammation, and postoperative symptoms. Propolis is a natural product that is potential as an anti-biofilm choice. The purpose of this study was to determine the ethanolic extract propolis (EEP) effect on the morphology of Staphylococcus aureus biofilm. The purpose of this study was to determine the effect of EEP on the morphology of Staphylococcus aureus biofilm. Isolate Staphylococcus aureus was taken from meatus medius of CRS patients in endoscopy sinus surgery at PHC Hospital Surabaya, Indonesia. Identification of Staphylococcus aureus uses Mannitol Salt Agar, Gram Staining, Catalase test, and Coagulase test. Biofilm produced from congo red agar culture. EEP was macerated from alcohol 70%, and after that the biofilm formed put in 24 well culture plate for 48 h using EEP solution dosages of 0.0%, 0.2%, 0.4%, 0.8%, 2.0%, 8.0%, 10.0% and negative control. Measurement intensity of expression Syto9 and the depth of biofilm using Confocal Laser Scanning Microscopy...
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Posted by admin on May 31, 2019 in |
Objective: The Indian traditional system of medicine, namely Ayurveda and Siddha, are primarily based on the use of the whole plant or different parts of plant singly or in combination to make multifactorial formulations. The use of plant drugs, however, demands correct identification of species and the characterization of phytoconstituents. Materials and Methods: The plants reported for their wound healing property in the Western Ghats by tribal community viz. Semecarpus anacardium L. (Bibba), Argemone mexicana L. (Firangi Dhotara), Cocculus hirsutus L. (Vasanvel), Woodfordia fruticosa Kurz. (Dhatki) were identified, authenticated and processed. The plant materials were subjected to morphological and microscopical evaluations. The proximate analysis (moisture content, ash values, extractive values and foreign organic matters) of powdered drugs were carried out. The powdered plant materials were extracted by a Soxhlet extraction process using different solvents. The concentrated extracts were subjected to preliminary phytochemical investigation. Results: The Semecarpus anacardium L. shows less moisture (fruits; 8.73 ± 0.95) and high inorganic content (leaves; 17.50 ± 0.92) as compared to other powdered drugs....
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