Posted by admin on May 31, 2019 in |
Overpopulation leads to rapid urbanization, and industrialization has directed an unplanned town expansion with no sanitary facilities and proper wastewater disposal arrangements drains in the pond. Mosquitoes create a significant public health problem in India and serve as vectors for transmitting various diseases like malaria, filariasis, dengue, chikungunya, etc., to humans. This results in the creation of water bodies which are highly conducive for the breeding of mosquitoes. The study revealed the biological activity of the compounds Capsaicin (C18H27NO3), and Phytol (C20H40O) from Capsicum annum L. docked against salivary gland protein (PMDBID PM 0079099). The Pharmacophore model development and 3D QSAR analysis of the selected compounds were carried out using the Pharmacophore alignment scoring engine (PHASE) module of Schrodinger. The 3D structures of proteins are of vital importance in providing insights into their molecular functions. The glide score of the compound Capsaicin was -2.63 and 2 two number of H-bond interactions with the distance of 2.298 and 1.485 and Phytol was -1.46 and one number of H-bond with a...
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Posted by admin on May 31, 2019 in |
Background: Bipolar disorder is a chronic mental illness due to a different interval of manic and depressive phase, the patient may become more prone to DRP, which interfere with the patients’ health outcome. Hence, clinical pharmacist can contribute to managing the DRP through proper pharmaceutical care intervention. Method/Design: A prospective, observational study had been done with 286 participants to assess, monitor, and resolve the DRP through Clinical Pharmacist intervention. Patients with Bipolar disorder, aged 18 to 65, included in the study except for the patient with other comorbid condition, lactating mothers, and pregnant women. Results: DRP in between, all the patients were observed, of which 70.9 % were drug-drug interaction followed by 29.1% ADR. The two major causes of DRP were at drug/dose selection level 95.5%, followed by a drug use process level 4.5%. Pharmacist intervention has been proposed at prescriber level, of which majority of interventions 52.7% had been accepted by the prescriber. Conclusion: In our study, the majority of DRP 64.7%, had been resolved due to the...
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Posted by admin on May 31, 2019 in |
Methanolic crude extract, diethyl ether fraction and sterol composition of Xestospongia muta were screened for cytotoxicity activity against HepG2 (liver hepatocellular) cells by MTS assay. Potential sterol mixture was profile using Gas Chromatography-Mass Spectrometry (GC-MS analysis), and luciferase assay were performed to further analyze its potentially on anti-atherosclerosis effects by increasing transcriptional activity of SR-B1 promoter. The result indicates that methanolic crude extract, diethyl ether fraction and sterol mixture (F2) does not exhibit cytotoxicity activity against HepG2 cell line with IC50 (>100 µg/mL, 94.45 ± 1.506 µg/mL and 55.30 ± 3.761 µg/mL). Cisplatin was used as a positive control, and the IC50 values against HepG2 cells were 3.22 ± 1.342 µg/mL. Sterol composition determines using GC-MS from diethyl ether fraction revealed 3 major sterols; cholesterol, stigmasterol, and ethanethioic acid. The mixture of different sterol analyses using luciferase assay showed potential on the ability to act as a ligand to activate the SR-B1 promoter. These preliminary findings suggested that sterol mixture from diethyl ether fraction is potential to be further...
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Posted by admin on May 31, 2019 in |
Tinospora cordifolia (Willd) Miers (Synonym: T. sinensis (Lour.) Merr.) is a genetically diverse plant known for its immense medicinal properties due to the presence of bioactive compounds among which alkaloids are most vital. So, the present study aimed to estimate the total alkaloid content in wild and in-vitro regenerated plant & callus tissue of Tinospora cordifolia. Different solvents, i.e., methanol, ethyl acetate, water, diethyl ether, and chloroform, have been tried and compared for their extraction efficiency. The presences of alkaloids were tested via Wagner’s and Mayer’s reagents. The quantitative estimation had been made by UV/Vis spectrophotometer using a dye-Bromocresol green. The methanol extracts of wild plant’s stem showed the highest concentration (3.3%) followed by water (1.85%) and ethyl acetate (0.99%). The alkaloid content in chloroform and diethyl ether extracts of wild plant’s stem was 0.49% and 0.28% respectively. The in-vitro regenerated plant extracts also showed the presence of alkaloids (though in minute amount) in all solvents viz. methanol (0.95%), diethyl ether (0.060%), chloroform (0.012%), water (0.80%) and ethyl acetate...
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Posted by admin on May 31, 2019 in |
The evaluation of acute oral toxicity of chloroform extract of Tephrosia calophylla (CETC) and methanolic extract of Tephrosia calophylla (METC) and sub-acute toxicity of CETC was done on albino Wistar rats. The animals (female) were divided into four groups containing 3 animals in each group normal control, and 200, 400 and 800 mg/kg of freshly prepared extracts respectively, every 24 h orally for 28 days. At the end of each study, physical parameters and hematological analysis were evaluated. There was no significant difference (p>0.05) observed with control in terms of body weights, food and water intake, hematological parameters, and gross abnormalities. No mortality was recorded. Hence, the study was concluded as oral administration of CETC for 28 days does not cause toxicity. In conventional therapy, various studies have been done to identify antioxidants from the plant sources. Excessive production of free radicals causes direct damage to the biological molecules such as DNA, proteins, lipids, carbohydrates leading to progression and tumor development. Phytochemicals from the plant origin serve as natural...
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