Posted by admin on Jul 31, 2019 in |
Fragaria ananassa fruit are small shrubs belonging to family rosaceae. Fragaria ananassa mainly contains anti-oxidants such as tocopherols, ascorbic acid and b-carotene which are effective against cancer, heart disease and cataracts. The focus of the study was toanalyze the diuretic activity of hydro alcoholic extract of Fragaria ananassa fruit in albino rats. Diuretic activity of hydroalcoholic (70:30) extract of Fragaria ananassa fruit (100mg/kg and 200mg/kg body weight orally) was studied in male wistar albino rats (n=5).Group I Serve as control receive 1% CMC solution, Group II serve as standard receive Furosemide 10mg/kg, Group III receive 100mg/kg Fragaria ananassa and Group IV receive 200mg/kg Urine volume at various interval was measured using metabolic cages (Lipschitz Method). The concentration of Na+, K+ in the urine at the end of 24 hours was estimated using flame photometer. One-way ANOVA technique was used for statically data analysis by which was followed by Dunnet t test (Graph Pad Prism software). On the basis of analysis, it was found that hydroalcoholic extract of Fragaria ananassa...
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Posted by admin on Jul 31, 2019 in |
Single-walled carbon nanotubes (SWNTs) though emerged as a promising material for delivery of biomolecules into various cells, due to their high cytotoxicity, they are limited in use in many biological systems and also in humans. The present research explores the preparation of functionalized SWNTs of low cytotoxicity and biocompatibility by altering the size and surface functionalization. Noncovalent amine functionalization improves their biocompatibility, solubility and alters their cellular interaction pathways resulting in reduced cytotoxic effects so that the biomolecule will be preferably released from the carbon nanotube when the complex has been taken up by endocytosis into the cells. The noncovalent functionalization of SWNTs was carried out by using polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol) – 2000] (ammonium salt) (DSPE-PEG 2000 amine) which makes the hydrophobic lipid chains of PEG to get strongly anchored onto the nanotube surface, whereas the hydrophilic chain renders SWNTs water soluble and biocompatible which results in the development of NH2-PEG-SWNTs. The functionalized SWNTs were further characterized by FE-SEM, Raman spectroscopy, FT-IR and UV-VIS-NIR, and zeta potential. So...
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Posted by admin on Jul 31, 2019 in |
Klebsiella pneumoniae is a significant pathogen for nosocomial infections and quickly becomes the most prevalent beta-lactamases produced that are resistant to several antimicrobials. To comprehend deeper the condition of multidrug resistance [MDR]. Objectives: Our research was directed at identifying Klebsiella pneumoniae isolates containing shv β-lactamase gene and the relationship between them and some antimicrobial resistance in order to prevent rejection of therapy. Methods: Overall, 64 K. pneumonia it gathered pneumonia isolates and conducted a drug susceptibility test for 13 different antibiotics by the system Vitek 2 to identify their susceptibility patterns. Phenotypic tests for species producing ß-lactamase have determined the resistance phenotype of isolates. Results: The information indicated that 60[93.7%] of the entire isolates tested were Shv genes, while the remainder were positive. The PCR was used to amplified drug resistance. Highest antibiotic resistance in instances of ESBL positive. In fact, 2[33.3%] of the phenotypical confirmatory Test [PCT] sample were shown to be negative isolates. The data has shown that prediagnosed positive phénotype isolates were genetically positive for EsBL development. Concept: the...
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Posted by admin on Jul 31, 2019 in |
Tectaria macrodonta C. Chr. locally known as Kaali negro in Sikkim is a rhizome and spore-bearing terrestrial fern, traditionally used in folk medicine. In the present study, the aqueous, ethanol and methanol extracts of the rhizomes of Tectaria macrodonta C. Chr were evaluated for its potential antibacterial and antioxidant properties. The phytochemical analyses of different solvent extracts were done. Antibacterial activity was determined by the agar well diffusion method against some gram-positive and gram-negative bacteria. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the plant extracts against the test bacteria were determined using the broth dilution method. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, ferric reducing power assay (FRAP) and hydroxyl radical (OH) scavenging assay. Potential antibacterial and antioxidant activity were exhibited by the methanol and ethanol extracts of Tectaria macrodonta. The methanol extract was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The results of the present study suggest the potential pharmaceutical importance of Tectaria macrodonta C. Chr. concerning its antibacterial...
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Posted by admin on Jul 31, 2019 in |
Paclitaxel is one of the best anti-cancer drugs and recently known as the best anti-cancer products of natural origin. The purpose of the present work was to study the physicochemical properties and compatibility of paclitaxel with the selected surfactants employed in nanocrystal formulations. The melting point and loss on drying of paclitaxel were found 216 °C and 0.11% respectively. A log P value of paclitaxel in different solvent systems like Octanol-Water, Dichloromethane-Water, Hexane-Water, and Oleyl Alcohol-Water was found 2.66, 2.91, 3.02 and 3.42 respectively. Paclitaxel is insoluble in water, soluble in ethanol, dichloromethane, and DMSO (dimethyl sulphoxide). The compatibility analysis of Paclitaxel with Egg lecithin, Poly Vinyl Pyrrolidine (PVP) and Poly (L‐lactic acid‐co‐glycolic acid) (PLGA) was studied by employing Fourier transform infrared spectroscopy (FTIR), X-Ray Powder Diffraction (XRPD) and Differential Scanning Calorimetry (DSC) techniques. FTIR, XRPD and DSC methods applied to the mentioned physical mixtures did not show evidence of interactions in the solid state. Based on these results supplied by FTIR, DSC, and XRPD, all the surfactants were...
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