Posted by admin on Jul 31, 2019 in |
The aim of current study was to compare the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of roots of seven Asparagus species from North-West India. Total phenolic content measured by Folin-Ciocalteu method ranged from 3.85 ± 0.25 (A. racemosus) to 7.74 ± 0.03 (A. falcatus), 3.42 ± 0.14 (A. racemosus) to 7.22 ± 0.12 (A. falcatus) and 1.12 ± 0.13 (A. officinalis) to 3.93 ± 0.54 mg/g of dry weight of root powder (A. retrofractus) in methanolic, ethanolic and aqueous extracts, respectively, expressed as gallic acid equivalents. The total flavonoid content determined by Aluminium chloride method ranged from 0.61 ± 0.03 (A. officinalis) to 1.86 ± 0.0303 (A. densiflorus), 0.39 ± 0.01 (A. adscendens) to 1.63 ± 0.0403 (A. densiflorus) and 0.31 ± 0.00 (A. adscendens) to 1.51 ± 0.02mg/gDW (A. densiflorus) in methanolic, ethanolic and aqueous extracts, respectively expressed as rutin equivalents. The results showed that TPC and TFC values were highest in methanolic extracts followed by ethanolic and lowest in aqueous extracts. The...
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Posted by admin on Jul 31, 2019 in |
The present study aims to formulate colon targeted drug delivery of Metronidazole compression coated tablets by using different ratios of chitosan and pectin. Carbopol 934P coating is given for compression coated tablets which makes them able to release the drug at the pH of the colonic fluid. Core tablets of Metronidazole (400 mg) were prepared by using swellable and pH dependent polymers like chitosan and pectin in which PVP-K30 is used as a binder. Drug release profile was evaluated in simulated gastric fluid, intestinal fluid, and simulated colonic fluid. The results of drug release studies performed according to the USP paddle method by using 0.1N HCl for 2 h, pH 7.4 phosphate buffer for 3 h and pH 6.8 phosphate buffer up to 24 h without using rat caecal content. Compression coated tablets containing chitosan as polymer released 95-99% of Metronidazole in a simulated colonic fluid, whereas tablets containing pectin as polymer released 94-99% of Metronidazole. The stability study for prepared tablets at 40 °C / 75% relative humidity...
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Posted by admin on Jul 31, 2019 in |
The purpose of the present study was to explore the effect of co-administration of bioenhancer quercetin on membrane permeability of poorly permeable berberine chloride, on goat intestinal membrane model. The effect of co-administration of quercetin was investigated at 2, 6, and 10 mg concentrations. The study revealed a beneficial effect of low concentration of quercetin on % cumulative drug release (% CDR) of the drug under treatment. The co-administration process resulted in remarkable improvement in the permeability of berberine chloride (% CDR 28.33 ± 1.87) at 2 mg of quercetin. On the contrary, the permeability of berberine chloride was decreased (% CDR 10.46 ± 1.55) at 10 mg concentration of quercetin as compared to berberine chloride alone (% CDR 8.49 ± 1.45 at 10 mg). Apparent permeability coefficient, flux, and enhancement ratio were also found to be increased significantly with decreasing concentration of quercetin as compared with the control. It could be concluded that the use of quercetin will be beneficial for co-administration to enhance the permeability, bioavailability, and...
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Posted by admin on Jul 31, 2019 in |
Introduction: Antioxidant properties of flavonoids may guard against oxidative damage to cells, DNA, or lipids. The ample literature about flavonoids can affirm the structural and pharmacological cherishing of this class of phytochemicals. The presence of aromatic rings in the flavonoid molecule consent the receiving and donation of electrons from free radical species, which abets to curb the free radicals. Methodology: This is a prospective observational study conducted on diabetic patient’s over-period of one year in the outpatient department of a tertiary care teaching Hospital. Red blood cells (RBC) were separated from the 20 ml of blood samples, which have been collected from the 90 subjects. The collected RBC were divided into four equal parts for which superoxide dismutase, catalase, and glutathione peroxidase activity was estimated with and without the flavonoids. The absorbance was recorded in a spectrophotometer. Results and Discussion: The results symbolize a significantly higher superoxide dismutase, catalase, and glutathione peroxidase activities for quercetin than kaempferol and chrysin. Quercetin is more effective on Superoxide dismutase followed by kaempferol and chrysin. But there...
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Posted by admin on Jul 31, 2019 in |
A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of Aspirin (ASP) and Omeprazole (OMP). By using box benchen design method have been developed and optimized. Effective chromatographic separation achieved using C18 column (250 × 4.6 mm, 5 µm) as a stationary phase and mobile phase consisted of methanol: Disodium hydrogen phosphate buffer (68: 32 v/v), pH adjusted to 4.5 with phosphoric acid at a flow rate 1.15 mL/min at a detection wavelength of 280 nm. Analytical Quality by Design approach was applied to evaluate the effect of three factors are the volume of the organic phase in the mobile phase, pH of mobile phase and flow rate on chromatographic responses like retention time and tailing factor. The retention time of ASP and OMP were found to be 2.94 and 5.87 min respectively. Calibration curves were found to be linear over the concentration range of 10-60 µg/mL for ASP and 5-30 µg/mL for OMP. The % recovery of...
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