Posted by admin on Apr 30, 2019 in |
A simple, specific, accurate and precise and stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Amlodipine Besylate and Valsartan in pharmaceutical formulation. The method was developed using Enable C 18G column (250 × 4.6 mm, 0.5 µm) with a mobile phase consisting of sodium acetate buffer (pH 5.0) and methanol (35:65% v/v) with a flow rate of 1 mL/min. The UV detection was carried out at 234 nm. The retention time for Amlodipine Besylate and Valsartan were found to be 3.146 and 6.543 min respectively. The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was observed over a concentration range 0.5-250 µg/ml for amlodipine besylate (r2 = 0.9996) and 1-90 µg/ml for Valsartan (r2 = 0.9984). The % RSD for Intraday and Interday precision was found to be 0.37 and 0.57 for Amlodipine Besylate and 0.48 and 0.75 for Valsartan. The LOD and LOQ were found to be 0.01 µg/ml, and 0.04 µg/ml for Amlodipine Besylate and LOD...
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Posted by admin on Apr 30, 2019 in |
Background: In India, the respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections (17 to 32%) in the pediatric age group. Viral detection during the acute phase of infection is a critical step for care and prevention. Objectives: We sought to investigate the detection of RSV by rt-RT-PCR, conventional PCR and cell culture among pediatric patients. Methods: Throat/nasal swab collected from the children (0-60 months) either outpatients/in patients having a respiratory illness from 2 to 5 days. Results: Total 375 samples were collected during 2011-2014, out of these 43 (11.4%) were positive for RSV by rt-RT-PCR, 38 (10.1%) and 22 (5.8%) were positive by conventional PCR and cell culture respectively. Bronchiolitis and pneumonia were commonly present in RSV (p<0.005). The positivity of the RSV was higher in infants (0-6 months). Conclusion: The rt-RT-PCR is rapid and sensitive, may replace conventional methods for early clinical diagnosis. However, cell culture and conventional PCR will remain important for molecular epidemiology. Age was an important risk factor which affects...
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Posted by admin on Apr 30, 2019 in |
Portulaca oleraceae commonly known as Purslane and Portulaca quadrifida commonly known as chicken weed are consumed in salads, pickles, and soup in Asia, Africa, and Mediterranean region. In the traditional systems of medicine in Asia and Africa, they have been attributed to several pharmacological properties. Phytochemical investigations point to the presence of various beneficial constituents including flavonoids, tannins, alkaloids, polysaccharides, vitamins, unsaturated fatty acids among others. In this study, ethanolic extracts of P. oleraceae and P. quadrifida were prepared using microwave-assisted extraction. These extracts were used separately to synthesize silver nanoparticles, and their characteristics were studied using SEM, TEM, and FTIR analysis. The antibacterial activity was tested on different gram-positive and gram-negative bacteria using agar diffusion technique and measuring the zone of inhibition. The analysis of biogenic nanoparticles indicated the presence of chemical bonds between the surface of nanoparticles and phytochemicals. The nanoparticles were found to be mostly spherical of diameters ranging from 140 nm to 1.1 µm present in clusters. All the nanoparticle samples exhibited antibacterial activity against...
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Posted by admin on Apr 30, 2019 in |
The worldwide usage of medicinal plants have been rapidly improved due to their medicinal property. Endophytic microorganisms are recognized as a potential source of novel chemical molecules that might be useful in the treatment of various diseases. In the present study, the plant samples were collected from Muthathi Wild Life Sanctuary, Mandya. The purpose of this study was to isolate the endophytic bacteria and their antibacterial activity against few human pathogens. Isolation of endophytic bacteria was carried out on nutrient agar media. About 41 bacterial endophytes were obtained from 17 medicinal plants in the study area. Out of this, 5 bacterial endophytes inhibits the best antimicrobial activity against Enterobacter aeruginosa, Klebsiella pneumonia, Lacto bacillus, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus...
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Posted by admin on Apr 30, 2019 in |
In an attempt to identify potential new agents active against tuberculosis with Shikimate kinase as the target, a novel series of 2-phenyl- 3-(5-sulfanyl-1,3,4-thiadiazol-2-yl)-1,3-thiazolidin-4-one derivatives were synthesized by convenient one-pot three-component reaction of amine, aldehyde and mercaptoacetic acid on montmorillonite KSF clay as a solid acidic catalyst in good yields. The structures of the newly synthesized compounds were confirmed by IR, 1H-NMR and elemental analysis and were subjected for anti-tubercular activity by Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37Rv. Docking and ADMET studies were used to better describe the titled compounds as potential anti-tubercular agents. The compound, 2-(3,4-dimethoxyphenyl)-3-(5-sulfanyl-1,3,4-thiadiazol-2-yl)-1,3-thiazolidin-4-one(4j), was found to be the most active against Mycobacterium tuberculosis H37Rv with MIC of 1.6 μg/ml and good drug likeness and dock scores. Molecular docking study revealed that the molecules fit well into the cavity of Shikimate kinase. Also, the molecular properties and bioactivity scores for the synthesized compounds obtained by in-silico studies were found to be within the acceptable range defined for human use revealing their potential as possible...
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