Posted by admin on Jul 31, 2019 in |
The genus Euphorbia has about 2000 members making it one of the largest ones among the flowering plants. Euphorbia helioscopia L. As the chemical composition of the essential oil from Euphorbia helioscopia developed in Egypt has not yet been explored, along these lines the present to extricate the essential oil and investigate the oil chemical composition and antimicrobial activity. Essential oil from the whole inflorescence of the flowering plant, Euphorbia helioscopia Linn. was extracted by hydrodistillation method. The chemical composition of the obtained volatile oil was investigated by GC-MS. The major components include thymol (48.36%), Caryophyllene (23.57%), carvacrol (6.70%), caffeic acid (6.48%) and caryophyllene oxide (5.58). Minor components as translongipinocarveol (2.77%) and DL-malic acid (1.9%). Screening antimicrobial activity of the isolated volatile oil against four bacteria showed that it was active only against Bacillus subtilis with an inhibition zone of 11 mm ± 0.25 and MIC value 17 mg /ml. Gentamycin was utilized as a positive antibacterial control. The data of this study might be considered as the first...
Read More
Posted by admin on Jul 31, 2019 in |
One of the proposed groupings of the fluoroquinolones describes the excellent broad-spectrum activity forms an invaluable part of the present anti-infective armory of the clinicians. The fluoroquinolones are most significant weapons can be credited for saving more human lives than any other area of medicinal therapy. In this current research segment, the novel C-3 substituted fluoroquinolone scaffolds were designed, synthesized and elucidated by IR, H1NMR, and MS spectral data. The clinically banned drug gatifloxacin is acting as starting material used to obtain targeted compounds. The proposed scheme comprised of main cyclization by using phosphorous oxychloride, thiosemicarbazide, gatifloxacin. The former compounds were undergoing diazotization reaction followed by a coupling reaction with various tertiary amines. Further, all seven 3- substituted gatifloxacin derivatives (PGI-PGVII) were screened for antimicrobial and hypoglycemic activity. In the novel series of compounds PG III, PG IV, PG V exhibited good antimicrobial activity by inhibition of DNA gyrase enzyme whereas PG I, PG III, PG VI, PG VII were found to be the most active compounds with hypoglycemic...
Read More
Posted by admin on Jul 31, 2019 in |
Bacterial resistance and oxidative stress are an important etiology of chronic diseases. This is the reason why new alternatives were established to overcome the incidence of some pathologies. Thus, the present study was aimed to evaluate the antibacterial activity of the extracted oil against bacteria that causes nosocomial infections in neonatal intensive care by using disc diffusion method. The antioxidant activity was evaluated using various methods (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power assay, total antioxidant activity) and the content of total phenolic and flavonoids were determined. In the essential oil, the twenty-eight compound was identified by GC/MS analysis. The major components were: 1,8-cineol (26.76%), 1-α-pinene (8.63%), linalool (7.78%). The oil strongly reduced the DPPH radical (IC50 = 4. 4 µg/ml) and reducing of molybdate (0.53 ± 0.0027 mg Eq ascorbic acid/µg EO. The oil showed strong antibacterial activity against nosocomial bacteria tested. This study confirms the essential oil of laurus nobilis. L processes antibacterial and antioxidant properties...
Read More
Posted by admin on Jul 31, 2019 in |
Objective: The antiepileptic potential of the petroleum ether (PETO) and methanolic extracts (METO) of the whole plant (roots, leaves, stem, and bark) of Trema orientalis (family: Ulmaceae) was evaluated using different experimental models of epilepsy in rats and mice. Materials and Methods: The anti-epileptic activity of the PETO and METO was evaluated using Picrotoxin, Isoniazid, and NMDA induced convulsion models. The brain GABA was also estimated in Picrotoxin induced convulsion model. The amount of the Lupeol present in PETO was estimated by HPTLC. Results: Preliminary phytochemical screening of the extracts revealed the presence of phytosterols, triterpenoids, Fixed oils & Fats, Tannins, phenolics, flavonoids, and carbohydrates. The anti-epileptic activity of PETO and METO at doses of 250 mg/kg and 500 mg/kg showed a significant increase (p˂0.001) in the onset of convulsions in the models used. The extracts also showed a significant increase in the brain GABA levels in the doses used. The amount of lupeol present in PETO was estimated to be 8.76 µg/ml. Conclusion: The PETO and METO showed...
Read More
Posted by admin on Jul 31, 2019 in |
The validated analytical method was applied for the estimation of Crisaborole in aqueous and human plasma with Crisaborole-D4 as an internal standard by using UPLC-ESI-MS/MS. The chromatographic separation was achieved with 10mM ammonium acetate buffer solution (pH- 4.5): Methanol, (10:90) (% v/v) using the Xterra C18, 100 × 4.6, 5µ. The total analysis time was 2 min, and the flow rate was set to 0.5 ml/min. The mass transitions of Crisaborole and Crisaborole-D4 obtained were m/z 252.1 ® 222.1 and 256.1 ® 222.1. The standard curve shows a correlation coefficient (r2) greater than 0.999 with a range of 75.00-225.00 ng/ml using the linear regression...
Read More
