Posted by admin on Jun 30, 2019 in |
Arunachal Pradesh (AP), the North-eastern state of India, is a natural habitat of 550 species of orchids including 37 species of medicinal importance. Though these plants are used extensively by locals for various ailments, very few scientific investigations have been conducted to establish their pharmacological potential. In the present study, we selected Tropidia curculioides, a relatively unexplored orchid to evaluate its cytotoxic potential. As any investigation has not been conducted to evaluate the cytotoxic potential of the extracts or compounds, we performed screening of the compounds, previously isolated by our group for cytotoxic activity against three cancer cells viz., MCF-7, A549, and SiHa cells. Interestingly, all three compounds exhibited IC50 in the range of 30-50 µM. Further, we studied apoptosis inducing property and ROS mediated cell death with the most active compound. The result indicated that TcR-3, a gallic acid derivative possesses maximum activity with IC50 of 34.26 µM against A549 cells. The cell proliferation assay and apoptosis-inducing property measured by flow cytometry indicated that the compound has comparable...
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Posted by admin on Jun 30, 2019 in |
Syzygium cumini L. belongs to the family Myrtaceae is one of the widely used medicinal plants in the treatment of various diseases particularly diabetes. The present study has been focused on the synthesis of silver nanoparticles of leaf, seed and fruit extracts of S. cumini and to observe their antimicrobial activity. The synthesized nanoparticles were characterized by performing UV-Vis spectrophotometer, SEM and TEM. UV visible spectroscopic analysis revealed the Surface Plasmon Resonance (SPR), the final reaction product confirming the reduction of AgNO3 to silver nanoparticles. Spherical, triangular and cubical images are observed in SEM analysis of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. TEM analysis also revealed the formation of 5 to 20 sizes of silver nanoparticles with the presence of the layer around the particle. Subsequently tested antimicrobial activity of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. Nanoparticles from seed extracts showed more antibacterial activity on Pseudomonas, followed by Bacillus, Klebsiella, Staphylococci and E. coli. Seed extracts also...
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Posted by admin on Jun 30, 2019 in |
The emergence of antibiotic-multidrug resistant microorganisms is an essential issue in the health sector. Consequently, the exploration of new antimicrobial compounds from many promising resources is urgently required. Soft coral-associated bacteria are potential as antimicrobial compound producers. This study aims to get the best medium and fermentation conditions in the production of secondary metabolites having antibacterial activities and to identify antibacterial agents from a bacterium associated with soft coral. A soft coral sample was collected from Randayan Island, Indonesia, and was identified morphologically as Sarcophyton sp. One of the bacteria isolated from the Sarcophyton sp. showed antibacterial activities and identified as Bacillus sp. based on Bergey’s Manual of Determinative Bacteriology. The best antimicrobial activities of Bacillus sp. DA11 were carried out on medium 1/10 Zobell supplemented with 1% of glucose (1/10ZG) which were able to against all of the test bacteria except on a test fungus, C. albicans. The extract was prepared on medium 1/10ZG with liquid state fermentation without shaking showed the best condition to produce secondary metabolites...
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Posted by admin on Jun 30, 2019 in |
This study was carried out to evaluate the antiviral activities of methanol and aqueous extracts prepared from the flowers and stems of Spartium junceum L., used as herbal medicine in the treatment of gastric ulcer in Turkish folk medicine, against herpes simplex virus type 1 (HSV-1). The maximum non-toxic concentration of the extracts (MNTC) was determined against Vero cells by using the serial two-fold dilution of MNTC (specific for each extract). The cytotoxic activity of the extracts and their ability to inhibit the cytopathic effect (CPE) caused by the virus in tissue culture was evaluated by colorimetric XTT assay after three days of inoculation and incubation. 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) were determined using graph pad prism, and the selectivity index (SI) was calculated from the EC50 ratio of CC50. As a result of the research, the strongest antiviral activity against HSV-1 was determined from flower aqueous extract with 1290.88 μg/mL EC50 and 24.09 SI values, and this was followed by stem aqueous extract (EC50...
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Posted by admin on Jun 30, 2019 in |
Staphylococcus aureus, the facultative anaerobic and opportunistic Bacterium has been characterized as the pathogen with diversified molecular adaptability leading to the emergence of MDR. Advanced use of alternate antibiotics is increasing the scenario of the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) to Oxacillin Resistant Staphylococcus aureus (ORSA) after that generating associated health infections. Even though Staphylococcus aureus is being considered as one of the important pathogens for its combination of virulence including toxin-mediated multidrug resistance and invasiveness, staphylococcus food poisoning is of high on interest as this infection rate is increasing day by day. Bacteriophage therapy has been of interest due to the absence of secondary resistance and targeted attack and also the lack of recolonization of the pathogen. The present study aims to isolate and purify Staphylococcus aureus-specific bacteriophages from sewage samples and thereby expunging food poison, causing Staphylococcus aureus. On this aspect Staphylococcus aureus causing food poison were isolated from a stool sample of patients at hospitals, and the specific Bacteriophage was isolated from the sewage in...
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