Posted by admin on May 31, 2024 in |
Introduction: Antibiotics’ efficacy is declining over a period of time because of haphazard use in humans and animals. Empirical use of antibiotics and reduction in the new drug development strategies by pharmaceutical industry can lead to antibiotic resistance crisis in near future. Hence, identifying the common causative organism and their resistance pattern in the geographical region would be helpful in choosing appropriate antibiotics for the diseases. Methods: This retrospective study is done at GMERS Medical College and hospital, Junagadh, Gujarat, India. Bacterial isolates and antibiotic susceptibility data from May 2021 to May 2023 were studied from culture and sensitivity register maintained by the microbiology department. Data were analyzed by descriptive statistics. Results: Among 43 positive samples 62.8 % were male and 37.2 % were female. Major samples were of sputum 39.53% and urine 32.56%. Organisms identified were Klebsiella 37.2%, E. coli 30.2%, Pseudomonas 16.3%, Acinetobacter 4.65%, S. aureus 9.3% and Enterococcus 2.3%. E. coli (69 %) were resistant to ampicillin/sulbactam and co-trimoxazole. Klebsiella (75%) were resistant to tetracyclin and...
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Posted by admin on May 31, 2024 in |
It belongs to the group of medications called as anti-metabolites and is a fluoropyrimidine carbonate with anti-cancer properties. The chemotherapy drug capecitabine is taken orally and is used to treat metastatic colorectal and breast cancers. A review of the literature reveals several analytical techniques have been developed for Capecitabinein single and combined with other drugs. No QbD-assisted RP-HPLC method is available to estimate Capecitabine. So, present work describes the development and validation of QbD driven RP-HPLC method for Capecitabine. This method has been developed using Agilent 1100 series HPLC with ODS (water) column (150 × 4.5mm, 5 µm). Based on the RP-HPLC method development Mobile phase and Flow rate were selected as CAA and retention time, peak area, theoretical plates, tailing factor of drug were monitored using Design Expert 13.0.0.5. By applying CCD, 8 trials having 2 factors and 4 responses method had been selected for method development of capecitabine. The optimum method development was selected based on the criteria of Retention time, Peak area, Theoretical plates, Tailing factor....
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Posted by admin on May 31, 2024 in |
Glinus oppositifolius (L) is a medicinal plant as well as edible leafy vegetables that has long been utilized for its therapeutic effects. The goal of this work is to use Atomic Absorption Spectroscopy to determine trace elements in Glinus oppositifolius (L). Acid digestion was used as a flexible method and all elements were determined using Atomic Absorption Spectroscopy. Calibration curves were created using standard solutions. The average concentration of the elements, expressed as mg/kg dry weight of the sample, were found to be 205.55mg/kg for Zn, 113.7mg/kg for Mn, 3.75mg/kg for Cr and 78.8mg/kg for Ca. Among the analysed elements, maximum amount of trace elements was Zn followed by Mn, Ca, and Cr. This study sheds light on that the Glinus oppositifolius (L) plants potential nutritional and health-related benefits through their high trace element content present. Trace element levels are being monitored and managed. Moreover, it showed that the AAS method is a simple, fast, and reliable for the determination of elements in plant materials. The obtained results of...
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Posted by admin on May 31, 2024 in |
Background: Self-medication is of particular concern as the trend is increasing now-a-days, especially during the post COVID-19 pandemic. Without adequate knowledge, self-medication may lead to adverse drug reactions, or in extreme cases, may require hospitalization for further management. Methods: A questionnaire-based cross-sectional study was undertaken involving the dental undergraduate students in a private dental college in Haryana after it was validated. 150 dental students filled the questionnaire within the age group of 17-25 years. Results: In the present study, the data has been obtained from self-medication questionnaire. Out of 150 students, the females were 106 (71%) and the males were 44 (29%). Our study showed that 91% of the students practice self-medication. Females practiced more self-medication (96.23%) than male students (79.54%). The most common reason for self-medication were previous visit to the doctor, medicines present at home of the previous visit, to save time, to avoid visit to the OPD/doctor and to save expenses. The sources of drug information which the students preferred were from the pharmacy (37.3%). Paracetamol...
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Posted by admin on May 31, 2024 in |
Livamurtha, traditional polyherbal formulation possess hepato-protective, antibacterial, and antihepatotoxic properties used for treating liver disorders. This exploratory study was designed to evaluate the acute and sub acute toxicity effects of traditional formulation in albino mice based on the OECD Guidelines. 10g of formulation ”Livamurtha” was boiled in 500ml of water for 1 hour. The decocation (Filtrate) obtained was evaporated and later were reconstituted in water for further analysis. In acute toxicity study, healthy albino mice were chosen for study with starting single dose of 5mg/kg/body weight followed by single doses of 50,300 and 2000mg/Kg/body weight in separate group of each 3 animals. The animals were observed for 14 days and its physical activities were noted. In sub toxicity study, the aqueous extract of formulation was administered orally at repeated dose of 300 and 600 mg/kg body weight for 28 days. The animals were sacrificed on the 29th day for further haematological, biochemical and histopathological examinations were done. In acute toxicity, no behavioural changes, mortality and toxicity was observed in...
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