Posted by admin on Apr 30, 2019 in |
In the present study, we examined the effect of Silibinin on the accumulation of oxidative stress induced by MPTP by in-vitro. After decapitation, healthy rat brain was removed rapidly from the skull and rinsed with cold artificial cerebrospinal fluid (ACSF) which has been equilibrated with 95% O2 / 5% CO2 gas mixture. Group I brain was incubated in CSF serve as normal, Group II lenses brain was incubated in CSF and DMSO (10%) serve as disease, Group III brain was incubated with MPTP (1 ng/mL) Group IV brain was incubated in MPTP with bromocriptine (10 µg/mL), Group V brain was incubated in MPTP with Silibinin (10 µg/mL) Group VI brain was incubated in MPTP with Silibinin (20 µg/mL). After 1 h of incubation brain slices were homogenized in PBS buffer, pH 7.4 and estimated for protein content, LPO* and GSH as per the procedures. MPTP incubated brain LPO* levels were increased, and GSH activity decreased compared to normal group. Silibinin treated group brain LPO* levels were significantly decreased and...
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Posted by admin on Apr 30, 2019 in |
This article presents the development of a direct, simple and sensitive spectrophotometric method for the quantitative analysis of mebendazole (MBz), an anthelmintic medication in its pure and pharmaceutical forms. The method is based on the charge transfer (CT) interactions between MBz, an electron donor, and chloranilic acid (CA), 3,5-dinitrosalicylic acid (DNSA), and picric acid (PA), (all of which are p-acceptors). The products of the CT reactions exhibit new absorption bands at 409 and 310 nm for CA and DNSA in methanol, respectively, and at 402 nm for PA in dichloroethane. Following the optimization of the experimental conditions, linearity ranges for MBz concentrations of 2.00-120, 1.00-40.0 and 2.00-25.0 µg mL-1 were achieved for CA-MBz, DNSA-MBz, and PA-MBz, respectively. Furthermore, the limits of detection (LOD) and quantification (LOQ) were calculated. The method was used to determine the MBz concentrations of tablets with good accuracy and precision and without interference from common tablet...
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Posted by admin on Apr 30, 2019 in |
Introduction: Tracheal intubation is commonly facilitated by the use of muscle relaxants. Sevoflurane fulfills the criteria for successful rapid & smooth inhalational induction when muscle relaxants are contra-indicated. Aim: To compare the success rate of intubation characteristics, hemodynamic changes, the effectiveness of the trapezius squeezing test for an adequate condition for intubation. Method: A total of 150 patients of ASA grade I and II undergoing elective surgical procedures of aged 20-60 years were included in our study. Patients on MAO-inhibitors, antidepressants, and B-blockers were excluded. 50 patients in each group; were randomized to groups A, B, and C with sevoflurane dial concentration of 3%, 5%, and 8% respectively. Before induction, priming of the circuit with allotted group done with 3 times bag full and empty cycles. Once the trapezius squeeze test is negative, IPPV (intermittent positive pressure ventilation) was continued for another one minute. Mean arterial pressure (MAP) and heart rate (HR), jaw relaxation, body movements vocal cord position; complications were recorded for all patients. Results: The incidence of...
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Posted by admin on Apr 30, 2019 in |
Bacopa monnieri is a herb, known to possesses various pharmacological and medicinal properties. Aim: The objective of the study was to analyse the activity of Bacopa monnieri as an antipyretic, analgesic and anti-ulcerogenic agent. Methods: Indomethacin was used as the standard of comparison for this study. Animals were arbitrarily separated into three groups of six animals in each group. The first group was accorded saline, while the second group was administrated with the test drug Bacopa monnieri, and the third group received indomethacin. Hot plate test and acetic acid test were performed to analyze the analgesic activity of Bacopa monnieri. The antipyretic activity was evaluated by a yeast solution which was used to induce fever before administering the drugs. For the ulcerogenic activity, the rats were kept on a fast to induce ulcers and then the action of Bacopa monnieri was evaluated. HPLC analysis of the Bacopa monnieri sample was performed to evaluate the active components. Conclusion: It was possible to conclude that Bacopa monnieri showed significant analgesic, antipyretic...
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Posted by admin on Mar 31, 2019 in |
Phytosomes are recently introduced drug delivery system and novel botanical formulation to produce lipophilic molecular complex to improve absorption and bioavailability of phytoconstituents. The objective of the present study was to formulate and evaluate tablets of Carica papaya phytosomes. Solvent injection method was used for the preparation of phytosomes. Carica papaya phytosomes were characterized by particle size, Zeta potential, Scanning electron microscopy, Fourier Transform Infrared Spectroscopy, Differential scanning calorimetry, and in-vitro drug release. The results showed that the average particle size and zeta potential of the optimized phytosomes formulation were 135.8 nm and −36.7 mV. In-vitro drug release studies revealed that the cumulative % drug release of tablets of Carica papaya phytosomes was found to be 85.32%. Cytotoxic effect of aqueous extracts and phytosomes on human leukemia cell line K-562 was evaluated by performing Sulforhodamine B (SRB) assay. The GI50 value of aqueous extract and F2 was found to be 75.2 μg/mL and 48.4 μg/mL respectively. The result showed that the phytosomes exhibited better anticancer activity than Carica papaya...
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