Posted by admin on Apr 30, 2019 in |
A series of condensed Imidazo [2, 1-b] [1,3] thiazole Chalcones have been synthesized by Claisen–Schmidt condensation reaction. Imidazo thiazole Chalcones are associated with immense biological activities. Novel Imidazo thiazole-based chalcones were synthesized using Imidazo thiazole-3-carboxaldehydes and various acetyl ketones in PEG-400. The structures of the compounds were characterized by IR, 1H NMR and screened for their in-vitro antioxidant (DPPH and SOD) activity. DPPH free radical scavenging activity was given by the compounds 7a-j at with (0.5 mmol/L in methanol). The compounds having substituent hydroxyl, fluoro, methoxy, ethoxy, and fluoro as well as benzimidazole and a ferrocenyl moiety in chalcone structure (7a-j) shows enhancement in antioxidant activity. Particularly in compounds like 7e, 7f, 7g, and 7h show the moderate percentage and 7c show the strongest percentage of inhibition mainly at (C=1mM). Almost all the compounds indicate moderate to good antioxidant activity compared to that of standard ascorbic acid percentage antioxidant activity (1 mm) by DPPH and SOD as 44.18 and 74.07 respectively. The resultant absorbance was recorded at 517 nm after 30 min incubation...
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Posted by admin on Apr 30, 2019 in |
A lectin curcin was isolated from mature seeds of Jatropha curcas L. Seeds were collected locally and purified by affinity chromatography on guar gum column. The lectin content was found to be 1.3 mg/ml. Curcin agglutinated B+ve human RBC type showing galactose and N-galactosylamine specificity. Galactose sugar was found to be the most potent inhibitor of curcin hemagglutinating activity (minimum inhibitory concentration (MIC) ≤ 50 mM). The lectin was a glycoprotein with a neutral carbohydrate content of 0.26µg/ml. The molecular mass of protein was found to be 28 kDa similar to previous reports of separation by SDS-PAGE. Curcin was stable at pH range from 4-10 while for temperature range it was stable from 10 ºC to 80 ºC for 30 min. Alignment with other lectin proteins by BLAST tool resulted into its similarity to 17 entries with 10 proteins with 100% query coverage but only RIP (Ribosome Inhibiting Protein domain) was visible in search results while carbohydrate binding domain was...
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Posted by admin on Apr 30, 2019 in |
Ethnic peoples from Purulia district of West Bengal in India use a lot of plants for their primary health care without studying phytochemical analysis and biological activities in detail. Main objectives of the present study are to evaluate the antibacterial and antioxidant activities of the whole plant extract of Curculigo orchioides using polar and nonpolar solvents. Total phenol content (TPC) was determined by Folin-Ciocalteu assay using Gallic acid (GA) as standard and total flavonoid content (TFC) by AlCl3 method using quercetin as standard. The antibacterial screening was carried out by agar well diffusion method. Methanol extracts exhibit higher phenol (82.93 ± 2.74 mg of GA E/gm of the sample), flavonoid (48.41 ± 1.94 mg of QE/gm of the sample) and alkaloid (5.56 ± 1.56%) content in comparison to other solvents. Similarly, methanol extracts show higher free radicals scavenging property and there was a positive correlation between IC50 values with total phenol and flavonoid content. A positive correlation exists in total phenol and flavonoid with antioxidant activities. The plant contains...
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Posted by admin on Apr 30, 2019 in |
Brexpiprazole is an atypical antipsychotic drug indicated for the treatment of schizophrenia. Four novels, simple, precise and accurate spectrophotometric methods were developed for the determination of Brexpiprazole (Rexulti) in pharmaceutical dosage forms. The absorption maxima was found to be at 214 nm in method A (0.1N HCl) and shows linearity over the concentration range of 0.002-0.02 µg/mL with regression equation 15.4517x + 0.0221 (r2 = 0.9990). In Method B (Sodium acetate buffer, pH 4.5) the drug obeys Beer Lambert’s law (λmax214 nm) in the concentration range of 0.005-0.1 µg/mL with regression equation 23.457x – 0.0235 (r2= 0.9992). First derivative spectrophotometric methods (C and D) were developed in 0.1N HCl and sodium acetate pH 4.5 in which Brexpiprazole obeys Beer Lambert’s law 0.002-0.02 µg/mL and 0.005-0.1 µg/mL with regression equations 4.9605x + 0.0003 and 1.9990x – 0.0009 respectively. The proposed spectrophotometric methods were validated as per the ICH guidelines and can be applied for the determination of Brexpiprazole in pharmaceutical...
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Posted by admin on Apr 30, 2019 in |
Nymphoides hydrophylla (NH) is mainly an aquatic herb of Menynthaceae family and traditionally used to treat fever, insect bites, different ulcers, and also to wash skin, where parasitic affection occurs. In this study, methanolic extract of Nymphoides hydrophylla (NH) plant was examined for the determination of thrombolytic, antipyretic, Central Nervous System (CNS) and analgesic activities. The evaluation of thrombolytic potential, antipyretic, CNS and analgesic activities of the plant extract occurred by their clot lysis, temperature reducing, and depression-inducing and pain sensation decreasing ability respectively. The result from the study showed the thrombolytic activity 3.05 ± 0.70% to 26.45 ± 2.18% indicating the better thrombolytic activity at a lower dose. The maximum pyrexia suppression of 91.07% is observed for 400 mg/kg NH extract. In hole cross and open field tests, maximum 71.42% and 66.80% suppression of locomotor activity were observed with the methanolic NH extract (400 mg/kg) whereas suppression of the locomotor activity of the standard drug diazepam was 68.57% and 51.20% respectively. From the analgesic data, about 91.72% inhibition...
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