Posted by admin on Mar 31, 2019 in |
The present manuscript describes a new simple, specific, precise and accurate RP-HPLC development and subsequent validation for simultaneous estimation of Pyrantel pamoate and Febantel in their combined dosage form. The proposed method involves the utilization of a hypersil BDS C18 (150 × 4.6 mm, 5 µ) column, mobile phase containing acetonitrile: phosphate buffer in the ratio 85:15% v/v and 55:45% v/v with apparent pH adjusted and maintained to 3.5 using 0.01M orthophosphoric acid, pumped at a flow rate of 1.0 ml/min. The injection volume was 20 µl, and detection was monitored at 286 nm. The components were eluted by gradient mode. The peaks obtained were sharp and well resolved with retention times 4.5 min (Pyrantel pamoate) and 6.5 min (Febantel). The calibration curves were linear (r2=0.999) over the concentration range of 25-150 µg/mL for Pyrantel pamoate and Febantel both. Validations of the proposed method were carried out for its accuracy, precision, linearity, robustness LOD and LOQ according to ICH Q2 R1 guidelines. The statistical analysis was carried out and...
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Posted by admin on Mar 31, 2019 in |
Background: Studies on challenges of the visually impaired population during medicine utilization, their difficulties and the Pharmacist’s information assessment towards such population are very limited. Objective: To study the problems during medicine utilization among blind patients in Nepal. Methods: A cross-sectional descriptive study was carried out in 120 blind persons using a schedule at various blind associations, hostels, colleges, and a blind cricket tournament. Participants of at least 18 years and had taken 2 medicines were included in the study. SPSS was used for analysis. Results: We found 60% could read Braille alphabet with most were male (63.88%) population. We found 21.43% only had adherence in their medicines. Most people had faced problem while using liquid dosage form where 46.7% of people spilled drug during administration and even 12.5% administered the drug directly from the bottle. The information given by dispenser was found to be inadequate. We found 65.8% of participants didn’t get to know their medicine name, staggering 80% haven’t got their medicine expiry date, 60% haven’t known...
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Posted by admin on Mar 31, 2019 in |
Formulating the essential oils with the chemotherapeutic agents in nanocarrier may develop a potent effect on the cancer cells. A nanoemulsion consisting of the camphor oil (CAM-NE) and Ifosfamide (IFO)-loaded in CAM-NE (CAM-IFO) were physically characterized by the zetasizer and evaluated for in-vitro antiproliferative activity against MCF-7 breast and HeLa cervical cancer cell lines. The cell growth inhibition, cell morphological changes, and apoptosis of the cancer cells, subjected into the CAM-NE, CAM-IFO and the free-drug (IFO), were examined by the MTT assay, light microscopy, and DAPI stain, respectively. It has been found that the dispersed nanodroplets of CAM-NE had mean particle diameter and zeta potential of 34.975 ± 9.35 nm and -13.75 ± 1.06 mV, respectively, which had got enlarged to 96.235 ± 9.00 nm and -22.00 ± 0.49 mV, respectively, when IFO was incorporated (CAM-IFO). The results of the cell growth inhibition and cellular uptake have demonstrated that CAM-IFO has the highest cytotoxic effect on both tested cells when compared with CAM-NE and IFO. These findings suggest that...
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Posted by admin on Mar 31, 2019 in |
The present work involves the extraction of phytochemicals from the bark of the medicinal plant Hopea ponga, with various solvents and evaluation of their antimicrobial, anthelmintic and antioxidant activities by following standard methods. The antioxidant capacity of the plant extracts was measured by their ability to scavenge free radicals such as (a) DPPH (2,2-diphenyl-1-picrylhydrazyl), (b) ABTS (2,2’-azinobis-(3-ethylbenzenothiaziline-6-sulfonate), (c) FRAP (Ferric Reducing Antioxidant Power) (d) Hydrogen peroxide assay. The methanol extract exhibited, high antiradical activity against DPPH, ABTS with an IC50 value of 19.48 µg/ml, 25.22 µg/ml, respectively, higher content of phenols (195.11 ± 2.14 mg expressed as mg of Catechol equivalents/100 mg dried extract and highest anthelmintic activity among all tested extracts. The ethyl acetate extract exhibited high antiradical activity against hydrogen peroxide with an IC50 value of 38.00 µg/ml and exhibited significant activity against Streptococcus aureus with a maximum inhibition zone of 35.93 ± 0.12 mm for 1000 mg/ml. The present study suggests that the methanol extract may contain active compounds, which have effective antimicrobial, anthelmintic and antioxidant...
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Posted by admin on Mar 31, 2019 in |
Pyrazolines were prepared from the corresponding chalcones by using isonicotinylhydrazine as cyclizing agent. The synthesized compounds were characterized by spectral data and screened for antimicrobial activity. In-silico studies of physiochemical and drug-like properties revealed that these compounds have good bioavailability and drug-likeness properties. Compound PZ2 which exhibited good antimicrobial activity was incorporated into polymeric nanoparticles using PLGA polymer by solvent evaporation method. The prepared nanoparticles were characterized by particle size, entrapment efficiency (EE%), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and in-vitro release studies. Prepared nanoparticles were incorporated into different hydrogel systems, which were prepared by using Carbopol 934 and HPMC in different concentrations ratio 2:1, 1:1 & 1:2. Hydrogel formulations were evaluated for drug content, spreadability, viscosity and drug release studies. Hence, the studies confirmed that entrapment of pyrazolthe ine as antimicrobial drugs in nanoparticle systems has found to be an inventive alternative that improves therapeutic effectiveness and curtails undesirable side effects of the drugs. Further, incorporation of pyrazoline nanoparticles into hydrogel provides an additional benefit of...
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