Posted by admin on May 31, 2019 in |
Acute gastroenteritis (AGE) and acute respiratory tract infection (ARTI) are the most common causes of morbidity and mortality in young children worldwide. This study aimed to analyze the sensitivity and specificity of laboratory diagnostic methods. We compared to cell culture and polymerase chain reaction (PCR) technique for the detection of Human adenovirus (HAdV) in AGE and ARTI children below 6 years of age. We analyzed 1270 samples taken from children, consisting of 537 fecal specimens from AGE and 733 throat swabs from ARTI pediatric patients. Hep-2 cell line was used for the isolation of HAdV. These viruses were identified by targeting partial hexon gene from fecal specimens and throat swabs. The total positivity for HAdV in AGE patients was 5.96% by cell culture and 8.75% by PCR, so increasing the percentage of identification from 5.96% to 8.75% (p>0.08). In the case of ARTI patients, 4.64% were positive by cell, while 7.23% by PCR in the increased rate of detection of HAdV infection from 4.64% to 7.23% (p<0.04*). Compared to...
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Posted by admin on May 31, 2019 in |
Purpose/Aim: In the present study, we investigate the antilipidemic effect of the aqueous extract Cymbopogon citratus. Background: Lemongrass is an aromatic plant belonging to the Gramineae family. C. citratus has been cultivated over many years for medicinal purposes in different countries throughout the world. Earlier in research, it was reported that the elevated cholesterol concentration was significantly lowered in the animals given the plant extract. This reduction was found to be dose-dependent. We attempt to evaluate the antilipidemic effect of aqueous extract of roots and flower of C. citratus in mice. Methods: Aqueous extract of roots and flower of Cymbopogon citratus evaluated for antilipidemic activity in mice. Blood was collected by intracardiac puncture, under mild ether anesthesia, and the total serum cholesterol, HDL, and LDL of treated mice were examined. Results: we observed that there is a significant decrease in the cholesterol levels in mice administered with root and flower extracts, there was an increase in HDL level and decrease in the LDL level in groups treated with the...
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Posted by admin on May 31, 2019 in |
The aim of the present study is to develop and evaluate the immediate release tablet of Vilazodone by direct compression method. The superdisintegrant crospovidone (CP), croscarmellose sodium (CCS), sodium starch glycolate (SSG), and Locust beam gum were used for the immediate release of drug from the tablet. Optimization of superdisintegrant concentration that can control the release of the drug as the hypothetical release profile was based on the release profile of the prepared trials. The prepared tablets were evaluated for all pre-compression parameters. Evaluation parameters Like weight variation, the hardness of the tablet, friability, thickness, disintegration test, drug content uniformity, and in-vitro release studies were performed. The drug-excipients interaction was investigated by FTIR. All formulation showed compliances with Pharmacopoeial standards. Among all the formulations, a formulation containing croscarmellose sodium as a super disintegrant is fulfilling all the parameters satisfactorily. The study reveals that formulations prepared by direct compression F5 exhibits the highest dissolution using croscarmellose sodium showed faster drug release 96.57% over 30...
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Posted by admin on May 31, 2019 in |
The present study evaluates the antidepressant activity of Nyctanthes arbor-tristis (NT). In the present study antidepressant effects of hydroalcoholic leaf extract (250 & 500 mg/kg) (NTL), flower extract (250 & 500 mg/kg) (NTF) and combination extract (leaf+flower extract in equal ratio) (250 & 500 mg/kg) (NTC) of Nyctanthes arbor-tristis were evaluated by the use of forced swim test. The animals were divided into different groups and were given daily dosing for seven successive days. On the seventh day, the immobility time and locomotor activity were noted down. The locomotor activity was taken before and after dosing. Initial phytochemical investigations showed the presence of some active constituents which were important for the antidepressant activity. All extracts of NT & imipramine treated groups demonstrated a significant reduction of immobility time. There were also statistically remarkable differences in immobility time in groups treated with combination extract compared to groups treated with leaf and flower extract alone. All extracts of NT also showed slight increment in the locomotor activity but were not statistically...
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Posted by admin on May 31, 2019 in |
Although doxorubicin is used widely in animal models to induce cardiotoxicity, serial dose-dependent cardiac effects have not been investigated in experimental animal models to date. Therefore, the objective of this study was to find out the dose-dependent changes of doxorubicin-induced acute cardiotoxicity, both biochemically and histopathologically in Wistar rats. Wistar rats were divided into nine groups. Group 1: normal control; Groups 2-9: eight doses of doxorubicin (13, 14, 15, 16, 17, 18, 19 & 20 mg/kg, intraperitoneal injection, after 16 h fast). Animals were sacrificed on the 4th day, and blood was collected for the estimation of cardiac troponin I (cTnI), aspartate aminotransferase (AST) and superoxide dismutase (SOD) activities and heart tissues were collected for histopathological assessment. Mean cTnI concentrations of groups 1-9 were 0, 39.46 ± 1.8, 60.92 ± 2.7, 87.79 ± 1.8, 116.96 ± 2.7, 147.79 ± 2.3, 163.96 ± 1.5, 197.38 ± 2.3 and 221.54 ± 1.8 pg/mL respectively. In groups 1-9, mean AST activities were 38.90 ± 0.98, 42.23 ± 0.99, 44.07 ± 1.20, 50.77...
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