Posted by admin on May 31, 2019 in |
Biofilm in chronic rhinosinusitis (CRS) is the infection by bacteria, which is difficult to overcome. It has recurrent infections, mucosal inflammation, and postoperative symptoms. Propolis is a natural product that is potential as an anti-biofilm choice. The purpose of this study was to determine the ethanolic extract propolis (EEP) effect on the morphology of Staphylococcus aureus biofilm. The purpose of this study was to determine the effect of EEP on the morphology of Staphylococcus aureus biofilm. Isolate Staphylococcus aureus was taken from meatus medius of CRS patients in endoscopy sinus surgery at PHC Hospital Surabaya, Indonesia. Identification of Staphylococcus aureus uses Mannitol Salt Agar, Gram Staining, Catalase test, and Coagulase test. Biofilm produced from congo red agar culture. EEP was macerated from alcohol 70%, and after that the biofilm formed put in 24 well culture plate for 48 h using EEP solution dosages of 0.0%, 0.2%, 0.4%, 0.8%, 2.0%, 8.0%, 10.0% and negative control. Measurement intensity of expression Syto9 and the depth of biofilm using Confocal Laser Scanning Microscopy...
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Posted by admin on May 31, 2019 in |
Objective: The Indian traditional system of medicine, namely Ayurveda and Siddha, are primarily based on the use of the whole plant or different parts of plant singly or in combination to make multifactorial formulations. The use of plant drugs, however, demands correct identification of species and the characterization of phytoconstituents. Materials and Methods: The plants reported for their wound healing property in the Western Ghats by tribal community viz. Semecarpus anacardium L. (Bibba), Argemone mexicana L. (Firangi Dhotara), Cocculus hirsutus L. (Vasanvel), Woodfordia fruticosa Kurz. (Dhatki) were identified, authenticated and processed. The plant materials were subjected to morphological and microscopical evaluations. The proximate analysis (moisture content, ash values, extractive values and foreign organic matters) of powdered drugs were carried out. The powdered plant materials were extracted by a Soxhlet extraction process using different solvents. The concentrated extracts were subjected to preliminary phytochemical investigation. Results: The Semecarpus anacardium L. shows less moisture (fruits; 8.73 ± 0.95) and high inorganic content (leaves; 17.50 ± 0.92) as compared to other powdered drugs....
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Posted by admin on May 31, 2019 in |
Overpopulation leads to rapid urbanization, and industrialization has directed an unplanned town expansion with no sanitary facilities and proper wastewater disposal arrangements drains in the pond. Mosquitoes create a significant public health problem in India and serve as vectors for transmitting various diseases like malaria, filariasis, dengue, chikungunya, etc., to humans. This results in the creation of water bodies which are highly conducive for the breeding of mosquitoes. The study revealed the biological activity of the compounds Capsaicin (C18H27NO3), and Phytol (C20H40O) from Capsicum annum L. docked against salivary gland protein (PMDBID PM 0079099). The Pharmacophore model development and 3D QSAR analysis of the selected compounds were carried out using the Pharmacophore alignment scoring engine (PHASE) module of Schrodinger. The 3D structures of proteins are of vital importance in providing insights into their molecular functions. The glide score of the compound Capsaicin was -2.63 and 2 two number of H-bond interactions with the distance of 2.298 and 1.485 and Phytol was -1.46 and one number of H-bond with a...
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Posted by admin on May 31, 2019 in |
Background: Bipolar disorder is a chronic mental illness due to a different interval of manic and depressive phase, the patient may become more prone to DRP, which interfere with the patients’ health outcome. Hence, clinical pharmacist can contribute to managing the DRP through proper pharmaceutical care intervention. Method/Design: A prospective, observational study had been done with 286 participants to assess, monitor, and resolve the DRP through Clinical Pharmacist intervention. Patients with Bipolar disorder, aged 18 to 65, included in the study except for the patient with other comorbid condition, lactating mothers, and pregnant women. Results: DRP in between, all the patients were observed, of which 70.9 % were drug-drug interaction followed by 29.1% ADR. The two major causes of DRP were at drug/dose selection level 95.5%, followed by a drug use process level 4.5%. Pharmacist intervention has been proposed at prescriber level, of which majority of interventions 52.7% had been accepted by the prescriber. Conclusion: In our study, the majority of DRP 64.7%, had been resolved due to the...
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Posted by admin on May 31, 2019 in |
Methanolic crude extract, diethyl ether fraction and sterol composition of Xestospongia muta were screened for cytotoxicity activity against HepG2 (liver hepatocellular) cells by MTS assay. Potential sterol mixture was profile using Gas Chromatography-Mass Spectrometry (GC-MS analysis), and luciferase assay were performed to further analyze its potentially on anti-atherosclerosis effects by increasing transcriptional activity of SR-B1 promoter. The result indicates that methanolic crude extract, diethyl ether fraction and sterol mixture (F2) does not exhibit cytotoxicity activity against HepG2 cell line with IC50 (>100 µg/mL, 94.45 ± 1.506 µg/mL and 55.30 ± 3.761 µg/mL). Cisplatin was used as a positive control, and the IC50 values against HepG2 cells were 3.22 ± 1.342 µg/mL. Sterol composition determines using GC-MS from diethyl ether fraction revealed 3 major sterols; cholesterol, stigmasterol, and ethanethioic acid. The mixture of different sterol analyses using luciferase assay showed potential on the ability to act as a ligand to activate the SR-B1 promoter. These preliminary findings suggested that sterol mixture from diethyl ether fraction is potential to be further...
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