Posted by admin on Mar 31, 2019 in |
The concept of fast dissolving drug delivery system emerged from the desire to provide patients with more convenient means of taking their medication. It is difficult for many patients to swallow tablets and hard gelatin capsules. The main objective of the study is to develop a reproducible formulation of fast dissolving tablets of Lornoxicam already used the therapeutic molecule to enhance effectiveness and to avoid side effects (gastric irritation) of the drug. Different batches of tablets were prepared by direct compression method using a different concentration of superdisintegrants like gum karaya, chitosan, and fenugreek seed mucilage powder. Before compression pre-formulation studies were done which includes characterization of the blend and physical compatibility studies with excipients. Effect of change in super disintegrant and their concentration on the formulation was studied. Final batches were compared for the superiority of superdisintegrants in the formulation of MDT of Lornoxicam. Tablets were evaluated for weight variation, thickness, hardness, friability, drug content, in-vitro disintegrating time and in-vitro drug...
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Posted by admin on Mar 31, 2019 in |
This paper describes a new validated Reverse-Phase High-Performance Liquid Chromatography (HPLC) method for the simultaneous determination of two anti-cancer drugs, Daunorubicin and Cytarabine (Ara-C). A simultaneous determination method saves cost and time as both drugs can be injected into a single HPLC system without the need to change or re-equilibrate with a new mobile phase. The objective of the study is to develop a simultaneous determination method of two anti-cancer drugs, Daunorubicin and Cytarabine. The mobile phase consists of a mixture (55:45 v/v) of 0.1% OPA: acetonitrile at a flow rate of 0.8 ml/min, with a PDA detector at 240 nm. Separation was achieved on a kromosil C-18 column (5 µm; 250 mm × 4.6 mm) maintained at 30 °C temperature in a column oven. The method was linear between 7.25 µg/mL – 43.5 µg/mL for Daunorubicin and 16.2 µg/Ml – 97.5 µg/mL for Cytarabine. The limit of detection was 0.29 µg/mL for Daunorubicin, and 1.15 µg/mL for Cytarabine and the limit of quantification was 0.88 µg/mL for Daunorubicin...
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Posted by admin on Mar 31, 2019 in |
The objective of this work was to formulate and characterize microparticles containing piperine, and evaluate their activity against carbon tetrachloride (CCl4)-induced liver toxicity. Piperine microparticles were formulated by o/w emulsion solvent evaporation technique using poly-ε-caprolactone as a polymer. Four different microparticle formulations (PM1, PM2, PM3, and PM4) were prepared by varying the drug/polymer ratio. The particles were characterized for particle size, drug content, surface morphology, and in-vitro drug release. The pharmacokinetics and pharmacodynamics of the piperine formulations in male Wistar rats were evaluated following intraperitoneal administration, using piperine solution as reference. The hepatoprotective activity of the formulation was determined in a CCl4-treated rat model and also compared with piperine solution. Piperine microparticles were successfully prepared using o/w emulsion solvent evaporation technique. The microparticles sustained the release of the drug both in-vitro and in-vivo for up to 10 days and offered better pharmacokinetic properties than the free drug itself. Microparticle formulation tested in-vivo demonstrated better pharmacokinetics and pharmacodynamics compared to the reference. Drug levels in the liver were significantly higher...
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Posted by admin on Mar 31, 2019 in |
A simple and precise UV-Visible spectrophotometric method for the simultaneous estimation of Resveratrol and folic acid in fixed-dose combination (FDC) has been successfully developed and validated. A suitable FDC of Resveratrol and folic acid was formulated for the treatment of oral squamous cell carcinoma. The present method involves the application of simultaneous equations (Vierodt’s method) for quantification of both the drugs. Resveratrol and folic acid were found to have absorbance maxima at 303.4 nm and 280.2 nm respectively in simulated saliva pH 6.8: methanol (1:1). The linearity studies indicated a concentration range of 1-7 ppm and 7-13 ppm for Resveratrol and folic acid respectively. The high values of the correlation coefficient indicate good linearity of the calibration curve for both the drugs. The recovery of Resveratrol and folic acid was found to be between 95.368 ± 0.364% and 101.260 ± 0.270% for Resveratrol and 101.330 ± 5.834% and 105.229 ± 0.1.164% for folic acid. The recovery results indicated that this method was suitable for simultaneous estimation of Resveratrol and...
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Posted by admin on Mar 31, 2019 in |
Introduction: Measles virus (MeV) is still a common acute illness caused by viruses belonging to the family Paramyxoviridae and genus Morbillivirus. The objective of this study was to determine the prevalence of MeV in both vaccinated and unvaccinated children and consecutively, testing the efficacy of MeV vaccination among children in north India. Materials and Methods: A total of 323 blood samples were collected along with measles mumps rubella (MMR) vaccination history from children (0-15 years) manifesting fever and rashes. In serum samples, we employed the enzyme-linked immunosorbent assay (ELISA) to quantify the presence of IgM antibodies against measles virus infection. Results: Out of 323 serum samples 148 (45.8%) were found to be positive for MeV infections. Expectedly, In the vaccinated group we found the prevalence of MeV is 70 (47.3%), while in unvaccinated children occurrence of MeV infection is 78 (52.7%), showing a statistical significance between vaccination history and measles infection (P<0.0001*). In this study, 0-5 years old children have 93 (62.8%) MeV infection conversely, >5 years old age...
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