Posted by admin on May 31, 2019 in |
Epilepsy is a disorder of the central nervous system. A seizure occurs when the brain’s nerve cells misfire and generate a sudden uncontrolled surge of electrical activity in the brain. Seizures can be controlled with modern medicines and surgical techniques and are found to have so many side effects. Natural products from folk remedies are an alternative source of anti-epileptic drugs with better safety and efficacy profiles. Rubiadin is an anthraquinone glycoside isolated from roots of Rubia cordifoia (Manjishta) known for several pharmacological activities. The present study was aimed to assess the anti-epileptic activity of isolated rubiadin from Rubia cordifolia. The evaluation of anti-epileptic activity was carried out with maximal electric shock (MES) and Pentylene Tetrazole (PTZ) models. The epileptic seizure was induced in mice of either sex and the challenged animals treated with whole Rubia cordifolia root extract & isolated Rubiadin suspension at two doses 100 mg & 250 mg respectively. The isolated Rubiadin suspension at 250 mg dose showed a significant reduction in MES and PTZ induced...
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Posted by admin on May 12, 2019 in |
In the present work, we have measured natural radioactivity in twelve building materials samples for four kinds (Brick, cement, gypsum and sand) obtained from some Iraqi governorates by using High Purity Germanium (HPGe) radiation detectors system. The results of measurements have shown that the average values specific activity concentrations for 238U, 232Th and 40K which were equal to (24.236 ± 4.4 Bq/kg), (26.394 ± 4.0 Bq/kg) and (174.888 ± 29.1 Bq/kg), respectively., which were lesser than the corresponding recommended global values reported by (UNSCEAR, 2000) publication. The radiation hazard indices [Raeq DƔ, (AED)in, (AED)out, Hin and Hex] were also studied. The obtained results were also found to be lesser than the allowed limits given by (UNSCEAR, 2000). Thus, all results obtained in the present work have shown no significant radiological hazard when the studied building materials is used for construction of...
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Posted by admin on Apr 30, 2019 in |
A simple, specific, accurate and precise and stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Amlodipine Besylate and Valsartan in pharmaceutical formulation. The method was developed using Enable C 18G column (250 × 4.6 mm, 0.5 µm) with a mobile phase consisting of sodium acetate buffer (pH 5.0) and methanol (35:65% v/v) with a flow rate of 1 mL/min. The UV detection was carried out at 234 nm. The retention time for Amlodipine Besylate and Valsartan were found to be 3.146 and 6.543 min respectively. The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was observed over a concentration range 0.5-250 µg/ml for amlodipine besylate (r2 = 0.9996) and 1-90 µg/ml for Valsartan (r2 = 0.9984). The % RSD for Intraday and Interday precision was found to be 0.37 and 0.57 for Amlodipine Besylate and 0.48 and 0.75 for Valsartan. The LOD and LOQ were found to be 0.01 µg/ml, and 0.04 µg/ml for Amlodipine Besylate and LOD...
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Posted by admin on Apr 30, 2019 in |
Background: In India, the respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections (17 to 32%) in the pediatric age group. Viral detection during the acute phase of infection is a critical step for care and prevention. Objectives: We sought to investigate the detection of RSV by rt-RT-PCR, conventional PCR and cell culture among pediatric patients. Methods: Throat/nasal swab collected from the children (0-60 months) either outpatients/in patients having a respiratory illness from 2 to 5 days. Results: Total 375 samples were collected during 2011-2014, out of these 43 (11.4%) were positive for RSV by rt-RT-PCR, 38 (10.1%) and 22 (5.8%) were positive by conventional PCR and cell culture respectively. Bronchiolitis and pneumonia were commonly present in RSV (p<0.005). The positivity of the RSV was higher in infants (0-6 months). Conclusion: The rt-RT-PCR is rapid and sensitive, may replace conventional methods for early clinical diagnosis. However, cell culture and conventional PCR will remain important for molecular epidemiology. Age was an important risk factor which affects...
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Posted by admin on Apr 30, 2019 in |
Portulaca oleraceae commonly known as Purslane and Portulaca quadrifida commonly known as chicken weed are consumed in salads, pickles, and soup in Asia, Africa, and Mediterranean region. In the traditional systems of medicine in Asia and Africa, they have been attributed to several pharmacological properties. Phytochemical investigations point to the presence of various beneficial constituents including flavonoids, tannins, alkaloids, polysaccharides, vitamins, unsaturated fatty acids among others. In this study, ethanolic extracts of P. oleraceae and P. quadrifida were prepared using microwave-assisted extraction. These extracts were used separately to synthesize silver nanoparticles, and their characteristics were studied using SEM, TEM, and FTIR analysis. The antibacterial activity was tested on different gram-positive and gram-negative bacteria using agar diffusion technique and measuring the zone of inhibition. The analysis of biogenic nanoparticles indicated the presence of chemical bonds between the surface of nanoparticles and phytochemicals. The nanoparticles were found to be mostly spherical of diameters ranging from 140 nm to 1.1 µm present in clusters. All the nanoparticle samples exhibited antibacterial activity against...
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