Posted by admin on Jun 30, 2019 in |
Syzygium cumini L. belongs to the family Myrtaceae is one of the widely used medicinal plants in the treatment of various diseases particularly diabetes. The present study has been focused on the synthesis of silver nanoparticles of leaf, seed and fruit extracts of S. cumini and to observe their antimicrobial activity. The synthesized nanoparticles were characterized by performing UV-Vis spectrophotometer, SEM and TEM. UV visible spectroscopic analysis revealed the Surface Plasmon Resonance (SPR), the final reaction product confirming the reduction of AgNO3 to silver nanoparticles. Spherical, triangular and cubical images are observed in SEM analysis of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. TEM analysis also revealed the formation of 5 to 20 sizes of silver nanoparticles with the presence of the layer around the particle. Subsequently tested antimicrobial activity of silver nanoparticles prepared from leaf, seed and fruit extracts of Syzygium cumini. Nanoparticles from seed extracts showed more antibacterial activity on Pseudomonas, followed by Bacillus, Klebsiella, Staphylococci and E. coli. Seed extracts also...
Read More
Posted by admin on Jun 30, 2019 in |
The emergence of antibiotic-multidrug resistant microorganisms is an essential issue in the health sector. Consequently, the exploration of new antimicrobial compounds from many promising resources is urgently required. Soft coral-associated bacteria are potential as antimicrobial compound producers. This study aims to get the best medium and fermentation conditions in the production of secondary metabolites having antibacterial activities and to identify antibacterial agents from a bacterium associated with soft coral. A soft coral sample was collected from Randayan Island, Indonesia, and was identified morphologically as Sarcophyton sp. One of the bacteria isolated from the Sarcophyton sp. showed antibacterial activities and identified as Bacillus sp. based on Bergey’s Manual of Determinative Bacteriology. The best antimicrobial activities of Bacillus sp. DA11 were carried out on medium 1/10 Zobell supplemented with 1% of glucose (1/10ZG) which were able to against all of the test bacteria except on a test fungus, C. albicans. The extract was prepared on medium 1/10ZG with liquid state fermentation without shaking showed the best condition to produce secondary metabolites...
Read More
Posted by admin on Jun 30, 2019 in |
This study was carried out to evaluate the antiviral activities of methanol and aqueous extracts prepared from the flowers and stems of Spartium junceum L., used as herbal medicine in the treatment of gastric ulcer in Turkish folk medicine, against herpes simplex virus type 1 (HSV-1). The maximum non-toxic concentration of the extracts (MNTC) was determined against Vero cells by using the serial two-fold dilution of MNTC (specific for each extract). The cytotoxic activity of the extracts and their ability to inhibit the cytopathic effect (CPE) caused by the virus in tissue culture was evaluated by colorimetric XTT assay after three days of inoculation and incubation. 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) were determined using graph pad prism, and the selectivity index (SI) was calculated from the EC50 ratio of CC50. As a result of the research, the strongest antiviral activity against HSV-1 was determined from flower aqueous extract with 1290.88 μg/mL EC50 and 24.09 SI values, and this was followed by stem aqueous extract (EC50...
Read More
Posted by admin on Jun 30, 2019 in |
Staphylococcus aureus, the facultative anaerobic and opportunistic Bacterium has been characterized as the pathogen with diversified molecular adaptability leading to the emergence of MDR. Advanced use of alternate antibiotics is increasing the scenario of the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) to Oxacillin Resistant Staphylococcus aureus (ORSA) after that generating associated health infections. Even though Staphylococcus aureus is being considered as one of the important pathogens for its combination of virulence including toxin-mediated multidrug resistance and invasiveness, staphylococcus food poisoning is of high on interest as this infection rate is increasing day by day. Bacteriophage therapy has been of interest due to the absence of secondary resistance and targeted attack and also the lack of recolonization of the pathogen. The present study aims to isolate and purify Staphylococcus aureus-specific bacteriophages from sewage samples and thereby expunging food poison, causing Staphylococcus aureus. On this aspect Staphylococcus aureus causing food poison were isolated from a stool sample of patients at hospitals, and the specific Bacteriophage was isolated from the sewage in...
Read More
Posted by admin on Jun 30, 2019 in |
Objective: To ensure the basic principles of drug utilization and the use and safety of blood thinning agents in tertiary care hospital. Experimental design: The study included 224 patients’ and was conducted in a tertiary care hospital and we were focused on to find out the drug-drug interactions of anticoagulants, antiplatelet, and thrombolytic agents, to study the major area involvement for blood thinning agents. The newly admitted case charts to the medicine ward will randomly be selected on a daily basis and will be reviewed for the medication safety compliance. Interpretation and Conclusion: Appropriate use of anticoagulant therapy and any deviation from the guidelines to a large extent also depends on patient characteristics and concomitant therapy patient is receiving. Some major complications are seen with oral anticoagulants which emphasize the need for a constant monitor and patient education. The need for dosage adjustments in different diagnostic situations or specific populations is very crucial. To increase patient education, an alert was built to notify pharmacists to educate patients on these...
Read More
