Posted by admin on Apr 30, 2019 in |
The worldwide usage of medicinal plants have been rapidly improved due to their medicinal property. Endophytic microorganisms are recognized as a potential source of novel chemical molecules that might be useful in the treatment of various diseases. In the present study, the plant samples were collected from Muthathi Wild Life Sanctuary, Mandya. The purpose of this study was to isolate the endophytic bacteria and their antibacterial activity against few human pathogens. Isolation of endophytic bacteria was carried out on nutrient agar media. About 41 bacterial endophytes were obtained from 17 medicinal plants in the study area. Out of this, 5 bacterial endophytes inhibits the best antimicrobial activity against Enterobacter aeruginosa, Klebsiella pneumonia, Lacto bacillus, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus...
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Posted by admin on Apr 30, 2019 in |
In an attempt to identify potential new agents active against tuberculosis with Shikimate kinase as the target, a novel series of 2-phenyl- 3-(5-sulfanyl-1,3,4-thiadiazol-2-yl)-1,3-thiazolidin-4-one derivatives were synthesized by convenient one-pot three-component reaction of amine, aldehyde and mercaptoacetic acid on montmorillonite KSF clay as a solid acidic catalyst in good yields. The structures of the newly synthesized compounds were confirmed by IR, 1H-NMR and elemental analysis and were subjected for anti-tubercular activity by Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37Rv. Docking and ADMET studies were used to better describe the titled compounds as potential anti-tubercular agents. The compound, 2-(3,4-dimethoxyphenyl)-3-(5-sulfanyl-1,3,4-thiadiazol-2-yl)-1,3-thiazolidin-4-one(4j), was found to be the most active against Mycobacterium tuberculosis H37Rv with MIC of 1.6 μg/ml and good drug likeness and dock scores. Molecular docking study revealed that the molecules fit well into the cavity of Shikimate kinase. Also, the molecular properties and bioactivity scores for the synthesized compounds obtained by in-silico studies were found to be within the acceptable range defined for human use revealing their potential as possible...
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Posted by admin on Apr 30, 2019 in |
The use of plants for contraception and abortion is recorded throughout history, with contemporary reports from India and many other countries. Daucus carota is one of the several plants traditionally used for birth control. The present work was undertaken to evaluate the antifertility effect of the seeds of the plant by carrying out pharmacological studies with the methanolic extract of the seeds. The seeds of Daucus carota were extracted with methanol. The dried methanol extract was administered orally to an experimental group of Swiss albino mice for 21 days. The duration of the phases of the estrous cycle, changes in the levels of reproductive hormones (LH, FSH, prolactin, estradiol, and progesterone) and the number of litters obtained were monitored and compared with the control group of the animals. Daucus carota seed extracts prolonged the estrous cycle of the experimental group of animals with a significant increase of the duration of the diestrus phase. The number of litters produced by the experimental animals was significantly less. Moreover, the extract caused an alteration in the hormone levels...
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Posted by admin on Apr 30, 2019 in |
Introduction: We investigated to an in-vitro and in-vivo evaluation of antimalarial and toxicity of the alkaloid-rich fraction of Momordica charantia fruits. Materials and Methods: In-vitro and in-vivo antimalarial activity tests of Plasmodium falciparum strain 3D7 culture were performed on Plasmodium berghei-infected mice using suppressive and prophylactic methods, and evaluation of fraction toxicity was conducted on zebrafish (Danio rerio). Results and Discussion: The study found that IC50 value of the in-vitro test was 0.17 ± 0.12 µg/mL and anti-malaria test using a suppressive and prophylactic method at a dosage of 100 mg/kg/day was found to inhibit parasites by 74 and 73%, respectively. The evaluation of toxicity by the use of zebrafish revealed abnormalities in the formation of the heart (51.28%) and yolk sac (51.28%). Conclusion: Alkaloid rich-fractions have powerful in-vitro and in-vivo antimalarial activity, but they have teratogenic effects that can inhibit the formation of the heart and yolk...
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Posted by admin on Apr 30, 2019 in |
Kinetics and stoichiometry S-oxidation reaction of sodium monohydrate Mezlocillin (Mezl) using potassium hydrogen peroxomonosulfate in aqueous solutions using iodometric titration were studied. Baypen®-powder Mezl sodium monohydrate in flacons for preparation of solution for injections (Mezl 1.0 g). A new iodometric method for the quantitative determination of sodium monohydrate Mezl in Baypen® preparation using potassium hydrogen peroxomonosulfate (KHSO5) as analytical reagent was proposed. Peroxomonosulfate acid as triple potassium salt 2КНSO5∙КНSO4∙K2SO4 (Oxone®) of “extra pure” qualification was used as the oxidant. At pH 1-4 for 1 mole of penicillin, 1 mole of KHSO5 is consumed, the quantitative interaction is achieved within a time of more than 1 min (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 2.01%, δ = (+ 0.51) %. It can be seen that Piperacillin can be determined successively with good accuracy and reproducibility. The new procedure was developed, and the ability of quantitative determination of penicillin in pharmaceutical preparation Baypen®...
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