Posted by admin on Apr 30, 2019 in |
Ethnic peoples from Purulia district of West Bengal in India use a lot of plants for their primary health care without studying phytochemical analysis and biological activities in detail. Main objectives of the present study are to evaluate the antibacterial and antioxidant activities of the whole plant extract of Curculigo orchioides using polar and nonpolar solvents. Total phenol content (TPC) was determined by Folin-Ciocalteu assay using Gallic acid (GA) as standard and total flavonoid content (TFC) by AlCl3 method using quercetin as standard. The antibacterial screening was carried out by agar well diffusion method. Methanol extracts exhibit higher phenol (82.93 ± 2.74 mg of GA E/gm of the sample), flavonoid (48.41 ± 1.94 mg of QE/gm of the sample) and alkaloid (5.56 ± 1.56%) content in comparison to other solvents. Similarly, methanol extracts show higher free radicals scavenging property and there was a positive correlation between IC50 values with total phenol and flavonoid content. A positive correlation exists in total phenol and flavonoid with antioxidant activities. The plant contains...
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Posted by admin on Apr 30, 2019 in |
Brexpiprazole is an atypical antipsychotic drug indicated for the treatment of schizophrenia. Four novels, simple, precise and accurate spectrophotometric methods were developed for the determination of Brexpiprazole (Rexulti) in pharmaceutical dosage forms. The absorption maxima was found to be at 214 nm in method A (0.1N HCl) and shows linearity over the concentration range of 0.002-0.02 µg/mL with regression equation 15.4517x + 0.0221 (r2 = 0.9990). In Method B (Sodium acetate buffer, pH 4.5) the drug obeys Beer Lambert’s law (λmax214 nm) in the concentration range of 0.005-0.1 µg/mL with regression equation 23.457x – 0.0235 (r2= 0.9992). First derivative spectrophotometric methods (C and D) were developed in 0.1N HCl and sodium acetate pH 4.5 in which Brexpiprazole obeys Beer Lambert’s law 0.002-0.02 µg/mL and 0.005-0.1 µg/mL with regression equations 4.9605x + 0.0003 and 1.9990x – 0.0009 respectively. The proposed spectrophotometric methods were validated as per the ICH guidelines and can be applied for the determination of Brexpiprazole in pharmaceutical...
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Posted by admin on Apr 30, 2019 in |
Nymphoides hydrophylla (NH) is mainly an aquatic herb of Menynthaceae family and traditionally used to treat fever, insect bites, different ulcers, and also to wash skin, where parasitic affection occurs. In this study, methanolic extract of Nymphoides hydrophylla (NH) plant was examined for the determination of thrombolytic, antipyretic, Central Nervous System (CNS) and analgesic activities. The evaluation of thrombolytic potential, antipyretic, CNS and analgesic activities of the plant extract occurred by their clot lysis, temperature reducing, and depression-inducing and pain sensation decreasing ability respectively. The result from the study showed the thrombolytic activity 3.05 ± 0.70% to 26.45 ± 2.18% indicating the better thrombolytic activity at a lower dose. The maximum pyrexia suppression of 91.07% is observed for 400 mg/kg NH extract. In hole cross and open field tests, maximum 71.42% and 66.80% suppression of locomotor activity were observed with the methanolic NH extract (400 mg/kg) whereas suppression of the locomotor activity of the standard drug diazepam was 68.57% and 51.20% respectively. From the analgesic data, about 91.72% inhibition...
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Posted by admin on Apr 30, 2019 in |
BCS class II drugs usually suffer inadequate bioavailability as a dissolution step is the absorption rate-limiting step. In this work, the effect of solubility increase at the main absorption site for these drugs was investigated using ritonavir as a drug model. The liquidsolid technique was applied to prepare ritonavir per-oral tablets of high dissolution rate using versatile non-ionic surfactants of high solubilizing ability [Transcutol HP, labrasol and labrasol/labrafil (1:1) mixture] as liquid vehicles at different drug concentrations (10–30%) and fixed (R). The prepared liquisolid tablets were fully evaluated, and the dissolution rate at pH 1.2 was investigated. The formula of higher dissolution rate was subjected to solid state characterization using Differential Scanning Calorimetric (DSC), Infrared Spectroscopy (IR). Results showed that liquisolid tablet prepared using labrasol/labrafil (1:1) mixture as a liquid vehicle containing 10% ritonavir is a compatible formula with a higher dissolution rate (100% in 35 min). This led to the conclusion that liquisolid technique efficiently improved the drug solubility and dissolution rate of BCS class II...
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Posted by admin on Apr 30, 2019 in |
This study aims to assess the preventive effects of green tea infusion and epicatechin on doxorubicin (Dox)-induced kidney and heart injuries and oxidative stress in male Wistar rats. The male Wistar rats administered Dox at an intraperitoneal dose of 4 mg/kg body weight (b.w.)/ week were orally treated with green tea infusion (200 mg/kg b.w.) and epicatechin (25 mg/kg b.w.) every other day for 6 weeks. The treatments of Dox-administered rats with green tea infusion and epicatechin resulted in a significant decrease in the elevated serum creatinine, urea and uric acid levels reflecting an improvement in kidney function. Similarly, the elevated serum CK-MB, LDH and AST activities were significantly ameliorated as a result of treatments of Dox-administered rats, thereby manifesting an amendment of heart function. The treatments also led to the prevention of the elevated lipid peroxidation and amelioration of the lowered GPx and GST activities as well as GSH content in kidney and heart. However, the SOD activity was not significantly altered in kidney and heart as a...
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