Posted by admin on Apr 30, 2019 in |
This study sought to evaluate the heavy metal content of the raw powder and extract of the plant, the phytochemical constituents, antioxidant effect by the use of DPPH assay, the total phenolic content using Folin Ciocalteu assay and the cytotoxic effect using the MTT Assay of Ficus pumila ethanolic extract, methanolic and hydro fractions on liver cancer cells (HepG2), Leukemic cells (Jurkat) and normal liver cells (Chang). FTIR and Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify the functional groups and major constituents of the most active fraction of F. pumila. Alkaloids, terpenoids, flavonoids, cardiac glycoside, saponins, and tannins were present in the ethanolic extract of F. pumila. The heavy metal analysis revealed the presence of Iron in both the raw powder (1.97 ± 0.11 mg/l) and extract (0.92 ± 0.02 mg/L). Zinc was also detected in both the raw powder (1.19 ± 0.00 mg/l) and extract (0.6595 ± 0.02 mg/l). The results from the FTIR revealed the presence of alkynes, alkyl halides, aromatics and aliphatic amines common to...
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Posted by admin on Apr 30, 2019 in |
Dipterocarpus gracilis Blume is one tropical species of Dipterocarps which is included in the red list IUCN as a rare and endangered tree species. In consideration of Dipterocarpus gracilis Blume conservation and sustainability of its oleoresin, this present study aimed to determine specific characteristics of oleoresin origin of Dipterocarpus gracilis Blume as an establishing basis for appropriate product diversification of human remedy. Here, is also evaluated the presence of different phytochemical along with GC-MS investigations of water and ethanol soluble crude extracts, and anti-oxidant obtained in oleoresin. The results showed that oleoresin of Dipterocarpus gracilis Blume revealed the presence of alkaloid and triterpenoid, whereas antioxidant activity test had low in 95% ethanol soluble. While, GC-MS analysis of organic compound of oleoresin of Dipterocarpus gracilis Blume identified that the most organic compound such as Caryophyllene, Caryophyllene oxide, Cyclohexanepropanal, 2,2-dimethyl-6-methylene-, 2, 6, 10, 14, 18, 22-, Tetracosahexaene, 2, 6, 10, 15, 19, 23-hexamethyl-, (all-E)-, 4-Pregnen-21-ol-3,20-dione glucoside, 1,4-Methanophthalazine, 1, 4, 4a, 7, 8, 8a-hexahydro-9,9-dimethyl-, (1α, 4α, 4a α, 8a α)-, and 9,...
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Posted by admin on Apr 30, 2019 in |
Marine organisms are excellent sources of structurally diverse molecules that are potentially valuable as drugs. The brittle starfish was shown to contain the bioactive components which exhibit various biological activities including cytotoxicity effects. Hence, the present study was carried out to determine the cytotoxicity of the extracts of two different starfish species like Ophiocoma erinaceus and Ophiomastrix annulosa. The homogenized fresh body component of brittle starfish was extracted and concentrated. The cytotoxic effect of EtOH extract of Ophiocoma erinaceus and Ophiomastrix annulosa against cancer cell lines was determined by MTT assay. In this study, it was observed that the extracts induce a concentration-dependent inhibition of cells against MCF -7, VERO and HELA cell line at a lower concentration. Based on abundance, the top three major compounds present in the O. erinaceus were n-Nonadecanol-1 (24.643), Ethyl 9-hexadecenoate (26.437) and hexadecanoic acid, ethyl ester (26.868). The starfish extract exhibited strong cytotoxicity against cancer cells. Overall, these findings confirmed the utility of clinical investigations of the efficacy of starfish extracts in preventing...
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Posted by admin on Apr 30, 2019 in |
To develop and validate simple, selective, specific, sensitive and accurate stability indicating assay method by RP-HPLC for determination of Loxapine succinate in the capsule dosage form. Several trials are taken by changing mobile phase composition, and ratio and finally the drug was eluted on a C18 Purospher star (250 mm × 4.6 mm, 5 µm) column using mobile phase consisting of water: Methanol: Acetonitrile: TEA:: 40:10:50:1 ratio and pumped at flow rate of 1.0 ml/min using 10 µl injection volume at 254 nm. The Retention time of Loxapine succinate was found to be 10.783 ± 0.02 min. The calibration curve was linear over concentration range of 75-225 µg/ml (r2 = 0.999). The accuracy of the method was estimated by recovery studies and % recovery was in a range of 98% to 102%. Stability of Loxapine succinate was studied under different stressed condition like acid, alkali, peroxide, thermal and photolytic conditions. The drug was degraded in all conditions except thermal and photolytic conditions. The developed method was validated as per...
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Posted by admin on Apr 30, 2019 in |
Introduction: Angiotensin-converting enzyme (ACE) inhibitors are a class of potential drugs used as first-line agents for the treatment of hypertension and heart failure in physiologically young patients despite its side effects. Apart from the other common side effects, they are known to induce bradykinin release which alters vascular permeability leading to pancreatic edema resulting in intrapancreatic entrapment of enzymes, toxins and acute pancreatitis. Objective: To determine the effect of long term ACE inhibitors use on serum amylase and lipase levels. Methods: A total of 122 patients were divided into two groups as case and control group, were case group the patients using ACE inhibitors for more than 8 months, and control group is the patients who are on other antihypertensive. Pancreatic enzyme levels were measured by ELISA method, and the elevation of pancreatic enzymes was determined in both the group and compared by Unpaired T-test. Results: Study showed a significant difference in amylase levels between case and control groups (P≤0.002) and Lipase level among case and control groups (P≤0.003)....
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