Posted by admin on Feb 28, 2019 in |
This study reports the potential of Alternanthera sessilis leaves as antioxidant, antimicrobial, anti-inflammatory and anti-diabetic agent and also the characterization of a novel phytochemical from A. sessilis using bio-activity guided extraction. Antimicrobial property was studied against five bacterial pathogens, Escherichia coli (MTCC: 1687), Bacillus cereus (MTCC: 0430), Klebsiella pneumonia (MTCC: 7028), Staphylococcus aureus (MTCC: 3160), Proteus mirabilis (MTCC: 3310) and against two fungal pathogens, Aspergillus niger [MTCC 281] and Aspergillus flavus [MTCC 10938]. Petroleum ether extract of A. sessilis leaves demonstrated significant antimicrobial property and hence was further purified to identify its active phytochemicals. Silica-gel Column Chromatography followed by antimicrobial screening, lead to the identification of the active fraction, that was characterized by spectroscopic analyses. FT-IR, GC-MS and NMR (C13, and DEPT-45; 90; 135) analysis of the active fraction, characterized the phytochemical to be 3,3’-(propane-2, 2-diyl)-bis-(3,4,5,6,7,8-hexahydro-1H-isochromene) abbreviated as PDHC. To the best of knowledge, this is the first report of PDHC due to novelty in its chemical structure. In-silico molecular docking was performed for this identified compound to identify...
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Posted by admin on Feb 28, 2019 in |
The main objective of this study is to evaluate the ability of production of xylanase by a fungal strain Penicillium sp. isolated from decaying wood. Xylanases are hydrolytic enzymes produced by many microorganisms which catalyze the hydrolysis of xylan polymer to xylose monomer. The xylanase production was carried out under submerged fermentation xylose as the main substrate, and the enzyme yield was regularly monitored. Maximum xylanase production was observed on the 4th day of incubation with 123.1 U/ml. The protein secretion was high on the 6th day with 787 μg/ml; total sugar was on the 7th day of incubation (982 μg/ml). Among natural substrates used in this study, corn cobs amended medium had yielded more xylanase production by Penicillium sp. after 6 days of incubation (167.2 U/ml) followed by sawdust and rice bran (128.1 and 120.8). The crude enzyme was used in bleaching of paper pulp and in biobleaching sample kappa number was reduced from 23.1 to 20.8 and brightness was increased to 40.1 from 39.2. It was also...
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Posted by admin on Feb 28, 2019 in |
Objective: Steroids are widely prescribed and used by practitioners due to powerful anti-inflammatory and immunosuppressive actions. So, care should be exercised in the rational selection of steroids. The main aim of our study is to assess prescribing patterns, demographic and clinical variables such as drug interactions associated with corticosteroid administration and steroidal use in a tertiary care teaching hospital. Methods: This is a prospective observational study conducted for 6 months in all departments of a tertiary care hospital of various age groups in all the departments were included. The study was carried out by taking 310 participants into consideration, and their prescribing patterns were observed and analyzed. Statistical procedure of One way ANOVA was done in SPSS version 16 software. Two way ANOVA was done which showed that variability is observed in the gender groups with treatment about steroids treatment. Results: Steroids were prescribed for various age groups of patients; 84 patients are above 60 years of age. Among 310, 183 patients were male and 127 patients were female....
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Posted by admin on Feb 28, 2019 in |
The purpose of this research was to obtain directly compressible agglomerates of Dantrolene using spherical agglomerates. Spherical agglomeration technique enables simultaneous crystallization and agglomeration. Sodium hydroxide acted as a good solvent, aqueous solution of PEG 6000 in hydrochloric acid served as bad solvent and isopropyl acetate was used as bridging liquid. The optimization of formula was carried out by using 23 factorial design, where the factors were speed of rotation, polymer: drug ratio and amount of bridging liquid. The responses evaluated were % drug release, MYP and Carr’s Index. The optimized agglomerates and Dantrolene was evaluated by powder X-ray diffractometry (PXRD) and scanning electron microscopy (SEM). PXRD showed reduction in crystalline nature of Dantrolene and SEM demonstrated spherical and smooth surface. The agglomeration improved the micromeritic properties of Dantrolene (Carr’s index16 and hausner ratio between 1.2 and mean yield pressure 3.58 tons). In-vitro dissolution increased by 10 fold. The AUC (35.19%), Cmax (23%) and tmax (reduced by 1 h) also recorded improvement. From the results, the conclusion is that...
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Posted by admin on Feb 28, 2019 in |
Piperine has been widely used as a bioenhancer. Secnidazole is a long-acting 5-nitroimidazole analog. It is used in the treatment of amoebiasis, giardiasis, trichomoniasis and anaerobic bacterial infection. In this study derivative of piperine was synthesized and studied for its bio enhancing the effect and this effect was compared with the bioenhancing effect of piperine. The pharmacokinetic profile of Secnidazole alone and in combination with piperine and piperine derivative was investigated by validated bioanalytical HPLC method as per USFDA guidelines. It was observed that the synthesized derivative of piperine significantly improved the bioavailability of Secnidazole compared to piperine alone in Wistar rats. The N,N-diallyl-5-(benz)(1,3)dioxol-5-yl)penta-2,4-dienamide (DA) derivative showed 1.49 fold increase in the bioavailability of Secnidazole. After oral administration of Secnidazole alone and in combination with piperine and piperine derivative (0.035 mmol, 10 mg/kg) there was a significant increase in AUC and Cmax of...
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