Posted by admin on Apr 30, 2019 in |
Diabetes Mellitus is a major lifestyle metabolic disorder and doubtlessly a challenging public health problem of the 21st century. Diabetes is associated with reduced performance on various domains of cognitive function, this study was aimed to access the effect of insulin on cognitive function in patients of Diabetes Mellitus. It was a prospective, observational and non-interventional study. 60 confirmed cases of Diabetes mellitus, who were recently (within 7 days) started on insulin were enrolled as study subjects from medicine department in a random fashion. Cognitive function was assessed by Addenbrooke’s Cognitive Examination (ACE III) at the time of enrolment, then 1 month and 3 months of insulin treatment. The results were analyzed using repeated measures analysis of variance (ANOVA) test. In results there was a significant increase in Attention, memory and visuospatial ability after one and three months, in language, there was substantial increase after one month but not after three months, and in verbal fluency, there was no significant increase after one and three months. Considering all the...
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Posted by admin on Apr 30, 2019 in |
This study is to evaluate two ethyl acetate fractions of “Tongkat Ali Roots” (TAR) namely fractions-1 and fraction-2 (F-1; F-2) on blood glucose levels in streptozotocin (STZ) induced diabetic rats. Each fraction used sixty rats which grouped into six groups (n=10). Group 1 is normal rats which received aqua dest; group 2 is diabetic rats which received STZ 60 mg/kg b.w., group 3 is diabetic rats with standard anti-diabetic metformin 10 mg/kg b.w. and group 4, 5, and 6 are diabetic rats which received tested fractions at doses of 25, 50 and 100 mg/kg b.w. Diabetic rats were induced by STZ intraperitoneally. The fasting blood glucose were assessed on days 0, 7th after diabetic inducing and 15th after giving tested fraction by using a digital glucometer (GCU Easy Touch) from the tip of the tail vein rat. The data were analyzed by using one-way analysis of variance (ANOVA). The fraction-2 (F-2) which was made from the extract of TAR by Vacuum Liquid Chromatography (VLC) using mobile phase ethyl acetate-ethanol-water...
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Posted by admin on Apr 30, 2019 in |
A new simple, precise, accurate and selective UPLC method has been developed and validated for stability indicating UPLC method for simultaneous estimation of Albuterol sulfate, Theophylline and Bromhexine HCl in the tablet dosage form. The method was carried out on a Hibra C18, 250mm x 4.6mm, 5µm. a column with a mobile phase consisting of buffer and acetonitrile and buffer in the ratio of (55: 45 v/v/v) and flow rate of 1.0 ml/ min. The detection was carried out at 260nm. The retention time for Albuterol sulfate, Theophylline, and Bromhexine HCl were found to be 5.8, 2.3 and 9.7 min respectively. The method was validated according to the ICH guidelines for specificity, LOD, LOQ, precision, accuracy, linearity and robustness. The method showed good reproducibility and recovery with %RSD less than 2. So the proposed method was found to be simple, specific, precise, accurate and linear than the methods reported earlier. Hence, the method is economical, and it can be applied for routine analysis of Albuterol sulphate, Theophylline and Bromhexine...
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Posted by admin on Apr 30, 2019 in |
Memory impairment (MI) is commonly seen in epilepsy and is at times aggravated by antiepileptic therapy. Recent studies reported that co-administration of nootropic agent with an antiepileptic drug (AED) might decrease the MI. This study was designed to evaluate MI activity of Eslicarbazepine (ESL) in temporal lobe epilepsy (TLE) and to assess the protective effect of Nefiracetam (NEF) which is a racetam group nootropic. MI activity was evaluated by radial arm maze (RAM) on lithium-pilocarpine induced TLE in Wistar Albino Rats. Antiepileptic activity of ESL was also assessed in the absence and presence of NEF. ESL treated a group of animals had increased errors in RAM, compared to the control group which indicated the presence of MI. The extent of MI was decreased when given a half dose of ESL. ESL aggravated the MI in epileptic rats which was very much similar to phenytoin, a standard more than the control group. Co-administration of NEF reversed the MI induced by ESL significantly. It was also observed that NEF treated animals...
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Posted by admin on Apr 30, 2019 in |
Lipases occupy a prominent place amongst biocatalysts as they can hydrolyze fats into fatty acids and glycerol at the water-lipid interface. Lipase production is a prime area of interest for microbiologists, process engineers, and biochemists. Recent advances in green energy have led to renewed interest in the ability of microbial lipases to catalyze trans-esterification reaction for biodiesel production. Research carried out in this field has revealed that microbes, especially fungi and bacteria, are the tools of choices for commercial production. The primary objective of the study was to isolate lipase-producing microbes from the waste product of oil-contaminated soil sample and to assess their ability to grow in different oil medium. Samples were collected from oil-contaminated garages in Hubli-Dharwad region, Dharwad, Karnataka. Lipid-degrading microbes were trapped in a specialized medium containing various types of oils. All isolated strains were morphologically, physiologically and biochemically characterized. Strain S4-4 was found to be the most efficient strain as it showed the highest lipase activity (25 μmoles/ml/min) after 24 h of incubation at 37 °C amongst the strains....
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