Posted by admin on Feb 28, 2019 in |
Objectives: Sickle cell disease (SCD) is a common hereditary blood disease with a progressive clinical course and multiple end organs damage. Due to its intercalated pathophysiology, several trials have been conducted targeting its pathophysiology in an attempt to modify the clinical course and minimize complications. Metformin and rosuvastatin are known to have pleiotropic effects such as antioxidant, anti-inflammatory and vasculoprotective properties making them a candidate for testing in SCD. Aim: To test possible effects of metformin and rosuvastatin on oxidative stress, erythrocytes deformability, %hemolysis in blood samples of patients with SCD. Materials and Methods: Blood samples were obtained from 36 SCD patients at stable conditions. The samples were incubated with metformin (1 mg/ml) or rosuvastatin (0.25 mg/ml). Oxidative stress was induced by incubating the samples with H2O2 for 30 min. Reduced glutathione (GSH), lipid peroxidation products (thiobarbituric acid reactive species TBARS), % hemolysis was determined by spectrophotometer and erythrocytes deformability by filtration method. Result: Treatment of blood with metformin or rosuvastatin resulted in a significant reduction in TBARS level...
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Posted by admin on Feb 28, 2019 in |
Present work has been conducted to evaluate antibacterial and antiviral activity of silver nanoparticles using marine Actinomycete, Nocardiopsis alba, isolated from mangrove soil. Formation, size, and shapes of silver nanoparticles (AgNPs) were characterized by UV-visible spectroscopy, X-Ray Diffraction (XRD), FTIR and Transmission electron microscope (TEM). From the UV-visible spectroscopy, the absorption peak was found at 420 nm. The SEM images confirmed that the sample contains spherical silver nanoparticles. The XRD analysis confirmed that the silver nanoparticles are crystalline, which was confirmed by the FT-IR peak at 564 cm-1 corresponding to the silver nanoparticles vibration present in the crystalline structure. The silver nanoparticles have significant antibacterial activity against Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus aureus, and E. coli. and antiviral activity against new castle viral disease (NDV) in cattle The findings of the present study suggest that the silver nanoparticles possess a good antibacterial a d antiviral activity and could have great importance as a therapeutic agent in current...
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Posted by admin on Feb 28, 2019 in |
Proteins play a significant role in living beings. In animals, where proteins are involved in the body built, the formation of tissues, organs, etc., in plants, proteins regulate the respiration, photosynthesis, growth, and reproduction. There are some proteins which are specific to the plant or a particular species of plant. Proteomics is a multidisciplinary branch of science that deals with the study of proteins. The paper discusses some important aspect related to estimation and identification of proteins. Protein was estimated in two plants of forensic importance, i.e., Ricinus communis and Cannabis sativa seeds by Ultra Violet (UV) spectrophotometry followed by isolation and characterization of proteins by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) method. A UV spectrum was taken at 520 nanometers (nm), and the protein was quantified by the Folin-Ciocalteu method. SDS-PAGE was performed according to Laemmli et al. High concentration of protein was found by UV spectrophotometry at 520 nm. SDS-PAGE separated the proteins according to their molecular weight ranging in size from 45 kiloDalton (kDa) to...
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Posted by admin on Feb 28, 2019 in |
Objective: This study aimed at ensuring the different compositions of Valli panchmoola using authentic herbs and establishing pharmacognostical, physicochemical, and phytochemical standards for the compositions. Methods: Compositions of Vallli panchmoola were prepared as per the formula and procedure mentioned in traditional texts and Ayurvedic Formulary of India. The prepared compositions were evaluated for pharmacognostical, physicochemical, and phytochemical parameters using guidelines of the World Health Organization and Pharmacopoeial Laboratory for Indian Medicines for quality control of herbal drugs. Results: Microscopic studies revealed the presence of collenchyma cells, rosette crystals, compound starch grains, parenchyma cells, cluster crystal, etc. Loss on drying 0.17 ± 0.01%, total ash 1.87 ± 0.02%, etc. were observed in the classical group. Whereas loss on drying 0.10 ± 0.04%, total ash 1.77 ± 0.02%, etc. were observed in group-1. Preliminary phytochemical screening confirms the presence of alkaloids, flavonoids, glycosides, fatty acids, terpenoids, sterols, tannins, proteins, and phenolic compounds. Conclusion: The various pharmacognostical, physicochemical, and phytochemical standards will help in quality control/quality assurance and maintaining batch to batch...
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Posted by admin on Feb 28, 2019 in |
The present research work was planned to formulate poloxamer 407 based hydrogel formulations of metronidazole and the evaluation of various parameters like swelling behavior, drug PH stability, in-vitro and in-vivo drug release, and in-vitro cytotoxic activity. Two different concentrations of metronidazole hydrogel formulations were prepared using poloxamer 407 and were assessed by a validated HPLC method for drug content, pH stability, and in-vivo drug release. Further, in-vitro anticancer activity was evaluated using sulphorhodamine B (SRB) assay in SCC29 cell lines. Both the formulations F1 and f2 showed better pH stability at pH 3.5, 5.5 and 6.8. The formulation F1 was able to absorb about 152% of its weight of water within 80 min, whereas F2 absorbed 167.4% of its weight of water and remains constant over 100 min. In-vitro and in-vivo drug release pattern showed half-life at 6 h, AUC0−t 692 and 684ng h/ml, Cmax 1059 and 1142 ng/ml for F1 and F2 respectively. Hydrogel formulation F1 showed improved percentage control growth when compared to F2 hydrogel formulation and...
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