Posted by admin on Feb 28, 2019 in |
Nanotechnology is an advancing technology expected to bring revolutionary changes in the field of life sciences including drug delivery. The advance in nanotechnology helps in the preparation of new pharmaceutical formulations with high benefits. One of its approaches is to stabilize the niosomal drug delivery system without affecting its properties have resulted in the development of proniosomes, a drug carrier. Proniosomes are solid colloidal particles which may be hydrated immediately before use to yield aqueous noisome dispersions similar to those produced by conventional methods. These proniosomes minimize the problems related to conventional niosomes such as aggregation, fusion, leaking and provide additional convenience in transportation, distribution, storage and dosing with enhanced stability. Ofloxacin loaded maltodextrin based proniosomes were prepared by a slurry method with a different surfactant to cholesterol ratio. The formulated proniosomes possessed good flow property and smooth surface confirmed by SEM study. FTIR spectra’s confirmed no interactions between drug and carrier. The niosomal dispersions were formulated and further evaluated for particle size, polydispersity index (PDI), zeta potential, TEM,...
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Posted by admin on Feb 28, 2019 in |
This study aimed to investigate the protective effect of flaxseed oil against hepato-renal toxicity induced by methotrexate. Rats treated with methotrexate exhibited elevations in the levels of AST, ALT, ALP, γGT, LDH, urea, creatinine, uric acid, and bilirubin. Furthermore, the levels of MDA, NO and the relative ratio of the gene expression of COX-2 and iNOS were significantly increased, accompanied by a decrease in the total protein, SOD, GPx, total thiol as well as the GSH content. Alterations in the lipid profile and hepato-renal histology were observed in rats treated with methotrexate. Also, up-regulation of α-SMA and loss of DNA bands integration were observed in methotrexate-treated rats. However, the oral treatment of flaxseed oil exhibited a protective effect against methotrexate toxicity in rats that could be attributed to its potent antioxidant, anti-inflammatory, and anti-apoptotic...
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Posted by admin on Feb 28, 2019 in |
Drug release properties from dosage forms can be modified by using many rate controlling polymers. Such controlled release properties from carrier systems are desirable since efficient drug delivery is achieved. The aim of the present study was to develop efficient chitosan nanoparticles loaded with rosmarinic acid (RA) and evaluate its drug release properties and wound healing efficiency in rats. Antimicrobial activity and wound healing effect of RA is well established and reported by many workers. Chitosan is also well known for possessing rate controlling properties. For proper application over wounds, the nanoparticles were incorporated into carbopol 940 hydrogel. A fourteen-hour in-vitro release study was carried out to evaluate the drug release efficiency and drug release kinetics of the nanoparticles. Other parameters evaluated were particle size, polydispersity index (PDI), zeta potential, morphology. Carbopol 940 hydrogel was evaluated for optimum viscosity (18.43 ± 1.7), swelling (250 ± 7.9) and spreadability (31 ± 1.5). The in-vivo study was carried out in three groups of animals, those treated with gel containing RA loaded...
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Posted by admin on Feb 28, 2019 in |
Development of quality assessment parameters for natural products is a prominence necessity to justify their acceptability and activity. Establishment of authentic and reliable analytical methods which profile the quantitative phytochemical composition of marker constituents in multicomponent composition like extract is a challenging task. A simple, rapid, precise, and reliable HPLC method was developed for the separation and estimation of ellagic acid from Epilobium angustifolium (Canadian willow herb) extract. The estimation was carried out using Sunfire C18 column, 5μ (4.6 × 250 mm); mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile; the flow rate of 1 mL/min and ultraviolet detection at 280 nm with a properly resolved having run time of 35 min. The method was validated as a final verification of method development concerning precision, linearity, accuracy, ruggedness, and robustness. The correlation coefficient (r2) > 0.999, a method is considered to be linear as the correlation coefficient was found to be within acceptance criteria. The % RSD of peak area response due to ellagic acid in five replicate injections of standard...
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Posted by admin on Feb 28, 2019 in |
In this study, radioprotection against radiation-induced hepatic damage in Swiss albino mice has been observed by Aloe vera. When individuals are exposed, the radiation energy is absorbed by the biological systems, which causes radiolysis of tissue water and generates free radicals. Animals were given Aloe vera leaf extract orally 1000 mg/kg body weight/day for 15 consecutive days before radiation exposure 0.5, 3 and 5.5 Gy gamma radiation. Mice were autopsied at day ¼, 1, 3, 5, 10 and 20 after irradiation to evaluate the radio modulator effect in terms of protein, cholesterol, and glycogen. In control set animals radiation treatment showed an increase of protein, glycogen, and cholesterol, however, the animals of experimental group, i.e., Aloe vera and radiation combined group showed a significant decrease in protein, glycogen and cholesterol but values remained below normal. It can be concluded that Aloe vera modulate the radiation-induced biochemical alterations in Swiss albino...
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