Posted by admin on Feb 28, 2019 in |
S. marcescens and P. mirabilis are gram-negative bacteria responsible for nosocomial infections. Quorum sensing regulates virulence in S. marcescens and P. mirabilis. Present study documents the potential of E. trigona extracts in inhibiting quorum sensing in both the bacterial species. Extracts of aerial part of the plant were prepared in petroleum ether, chloroform, ethyl acetate, acetone and methanol using soxhlet extraction technique. Petroleum ether extract, chloroform extract, and acetone extract were found to quench quorum in S. marcescens. These extracts were next subjected to column chromatography and assayed for its quorum quenching capacity. Later, thin layer chromatography was used to separate and identify the bioactive fraction responsible for quorum quenching. The fraction exhibiting quorum quenching capacity was subjected to GC-MS analysis. It revealed the presence of fatty acid derivatives in the bioactive fraction. A major part of the bioactive fraction was 9,12-Octadecadienoyl chloride (Z, Z), a derivative of linoleic acid, and, hence linoleic acid was also used for testing its effect on swarm motility of bacteria. Furthermore, the bioactive...
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Posted by admin on Feb 28, 2019 in |
Hyperin, a flavonoid is an active phytochemical constituent gift in numerous plants as well as, Hypericum perforatum, Drosera rotundifolia, Stachys byzantina, Prunella vulgaris, Rumex acetosella, Abelmoschus manihot and Rhododendron arboreum. It’s protecting result on cultured PC12 cells against toxicity induced by hydrogen peroxide and tert-butyl hydroperoxide. Hyperin, isoquercitrin and quercetin isolated from ethyl acetate fraction of the root of A. chiisanensis showed lipopolysaccharide-induced nitrite production in rat peritoneal macrophages. As a crucial bioactive compound, hyperoside (hyperin) has been documented to possess antihyperglycemic, antiviral activity, antinociceptive, medicament, cardioprotective, hepatoprotective, and gastric-mucosal protecting result. The current study evaluates the ulcer healing potential of hyperin against absolute ethanol- (necrotizing agent), aspirin- (non-steroidal medicament drug) and histamine- (gastric secretion stimulator via H2 receptor) iatrogenic ulcers in rats. Hyperin (25 mg/kg and 50 mg/kg) was administered orally to the long fasted rats, one hour before the absolute ethanol/ aspirin/ histamine challenge. The ulcer index, gastroprotective potential, the status of the inhibitor enzymes enzyme (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) along with reduced...
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Posted by admin on Feb 28, 2019 in |
Almost 85% of the world population is infected with the Herpes Simplex Virus (HSV). Herpes simplex virus infections have been described in the medical literature for centuries, yet the drugs available nowadays for therapy are largely ineffective and low oral bioavailability plays an important role on the inefficacy of the treatments. Additionally, the details of the inhibition of Herpes virus type 1 are still not fully understood. The present study was undertaken to evaluate the antiviral property of selected phytochemicals from Verbascum phlomoides L. through their interaction with the Herpes simplex virus. Depending on the non-covalent interaction and best docking score it was concluded that these phytochemicals may be useful for treatment against Herpes infection and can act as inhibitors to Herpesviral protein receptor. Also using phytochemicals as a medium of medication may reduce the cost dependent factor and can be widely used as medicinal purposes to treat several chronic and dreadful...
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Posted by admin on Feb 28, 2019 in |
The present study was designed to develop and validate a simple, sensitive, precise and accurate RP-HPLC method for simultaneous estimation of dicyclomine and ethylmorphine in bulk and tablet dosage form. The chromatographic separation was achieved on Discovery C18 column (250 × 4.6 mm, 5 µm) as stationary phase with a mobile phase of water (pH 5.4 adjusted with orthophosphoric acid): acetonitrile (40:60 v/v) at a flow rate of 1 ml/min and PDA detection at 215 nm. The proposed method was validated for system suitability, specificity, linearity, accuracy, precision, LOD, LOQ, and robustness as per ICH guidelines. The retention times of dicyclomine and ethylmorphine were found to be 3.166 ± 0.02 and 4.204 ± 0.19 min respectively. The calibration curves were linear in the concentration range of 50% to 150% of the working concentration (r2=0.999) for both the drugs in a binary mixture. The accuracy was found to be 98.61 % and 99.24 % for dicyclomine and ethylmorphine respectively. The LOD was found to be 0.05 µg/ml, and 0.20 µg/ml...
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Posted by admin on Feb 28, 2019 in |
Diabetes is a noncommunicable chronic metabolic disorder characterized by high blood glucose level in the body. One of the antidiabetic therapeutic strategies is the inhibition of carbohydrate-digesting enzymes such as α-amylase and α-glucosidase. In the present study methanolic extract and its two fractions (Ethyl acetate and n-butanol) of the whole plant of Trichuriella monsoniae were evaluated for their effect on α-amylase and α-glucosidase enzymes by using in-vitro assays. The plant extracts were used at varying concentrations to ensure which concentration of the extract/fractions of the plant causes the most inhibition. Phytochemical analysis of the extract and its fractions indicated the presence of flavonoids, steroids/terpenoids, carbohydrates, saponin, phenolic compounds. While tannins and alkaloids are absent. n-butanol fraction shown the prominent α-amylase and α-glucosidase enzyme inhibitory activities (IC50 4.09 mg/ml and 3.30 mg/ml respectively) than methanolic extract and its ethyl acetate fraction, and it was well comparable with the standard drug acarbose (for α-amylase IC50 3.62 mg/ml and for α-glucosidase IC50 2.19 mg/ml). Further, the Total phenolic and flavonoid contents were...
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