Posted by admin on Apr 30, 2019 in |
Bacopa monnieri is a herb, known to possesses various pharmacological and medicinal properties. Aim: The objective of the study was to analyse the activity of Bacopa monnieri as an antipyretic, analgesic and anti-ulcerogenic agent. Methods: Indomethacin was used as the standard of comparison for this study. Animals were arbitrarily separated into three groups of six animals in each group. The first group was accorded saline, while the second group was administrated with the test drug Bacopa monnieri, and the third group received indomethacin. Hot plate test and acetic acid test were performed to analyze the analgesic activity of Bacopa monnieri. The antipyretic activity was evaluated by a yeast solution which was used to induce fever before administering the drugs. For the ulcerogenic activity, the rats were kept on a fast to induce ulcers and then the action of Bacopa monnieri was evaluated. HPLC analysis of the Bacopa monnieri sample was performed to evaluate the active components. Conclusion: It was possible to conclude that Bacopa monnieri showed significant analgesic, antipyretic...
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Posted by admin on Mar 31, 2019 in |
Phytosomes are recently introduced drug delivery system and novel botanical formulation to produce lipophilic molecular complex to improve absorption and bioavailability of phytoconstituents. The objective of the present study was to formulate and evaluate tablets of Carica papaya phytosomes. Solvent injection method was used for the preparation of phytosomes. Carica papaya phytosomes were characterized by particle size, Zeta potential, Scanning electron microscopy, Fourier Transform Infrared Spectroscopy, Differential scanning calorimetry, and in-vitro drug release. The results showed that the average particle size and zeta potential of the optimized phytosomes formulation were 135.8 nm and −36.7 mV. In-vitro drug release studies revealed that the cumulative % drug release of tablets of Carica papaya phytosomes was found to be 85.32%. Cytotoxic effect of aqueous extracts and phytosomes on human leukemia cell line K-562 was evaluated by performing Sulforhodamine B (SRB) assay. The GI50 value of aqueous extract and F2 was found to be 75.2 μg/mL and 48.4 μg/mL respectively. The result showed that the phytosomes exhibited better anticancer activity than Carica papaya...
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Posted by admin on Mar 31, 2019 in |
In this paper Chromium (VI) based Pyridinium fluoro chromate (PFC) reagent has been used as an oxidant for the determination of some antihistamine drugs e.g. Promethazine hydrochloride (PMH) and Pheniramine maleate (PM) in pure form and their pharmaceutical preparations such as Phenergan (Injection and tablet) and Avil (Tablet and injection) respectively. The main principle of this method is based on the fact, that each pharmaceutical drug contains a specific organic functional group, which on oxidation in the presence of selected oxidant (Here PFC is used as an oxidant) provides the new oxidized product. This type of oxidation reaction between the drug molecule and an oxidant establishes a stoichiometric relationship between the drug molecule and an oxidant. This relationship is the basis of quantitative estimation of drugs in pure form and their pharmaceutical preparations. In this research, oxidizing reagent Pyridinium fluoro chromate (PFC) oxidizes the sulfur atom of Promethazine hydrochloride (PMH) to the corresponding sulphoxide, whereas in case of Pheniramine maleate (PM) it oxides one hydroxyl (-OH) group of carboxylic...
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Posted by admin on Mar 31, 2019 in |
It is well known that Trichoderma sp. can be used as a biological control agent (BCA). In this study, 02 new Trichoderma isolates were obtained from 35 different soil samples of Punjab, and Himachal Pradesh and its bio-control/antifungal effects were determined against 07 newly isolated postharvest fungal pathogens (PHFP) from 35 different samples of fruits and vegetables collected from storage and selling markets of Chandigarh. In-vitro evaluation of antagonistic activity of only one newly isolated bio-control agent Trichoderma viride (RS1) was done by dual culture method (DCM) and culture filtrate technique (CFT) and compared with the standard BCA, T. viride MTCC 800. Results of CFT were significantly higher than by DCM in case of newly isolated BCA in comparison to standard BCA. Further, a pot experiment was carried out to check the effect of fungal pathogens and bio-control agent on the growth of barley seeds. It was observed that in the presence of BCA alone as well as in combination with PHFP, the negative effect of fungal pathogens reduced...
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Posted by admin on Mar 31, 2019 in |
Simple, rapid validated stability-indicating HPLC method for estimation of Salmeterol xinafoate was successfully developed. The separation was achieved by using a mobile phase of buffer: methanol in the ratio of 60:40 v/v using HiQ SiL C18 column (150 × 4.6 mm i.d. 5 μm) at 1.2 mL/min as flow rate. The detection was carried out at 252 nm using a PDA detector. The retention time observed was 11.89 ± 0.3 min. This drug was subjected to stress conditions as per ICH Q1A (R2). Linearity was found to be in the concentration range of 10-50 μg/mL with r2 = 0.9958. The suitability of this HPLC method for quantitative estimation of Salmeterol xinafoate was proved by validation by the requirements of ICH guidelines Q2A...
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