Posted by admin on Jan 31, 2019 in |
Regular intake of carbohydrate diet containing high fructose or sucrose increases adipogenesis resulting in conditions like weight gain, high blood pressure, elevated blood glucose levels, insulin resistance, raised triglycerides, low HDL cholesterol, with metabolic syndrome as a leading consequence. Since intricacies of adipogenesis are of major relevance to human health; present study aimed to evaluate the morphological effects of trigonelline on mesenchymal stem cells, C3H10T1/2 treated with high fructose (equal to that in high fructose corn syrup) in comparison to the adipogenic cocktail. In this regard, cells were given trigonelline alone as well as in combination with an adipogenic cocktail or high fructose to evaluate its potential to affect adipogenesis morphologically. The cells of positive control (containing only adipogenic differentiation media) depicted morphological changes towards spherical structure, a feature definite of adipocytes. Similar observations were seen in cells treated with high fructose (1 mM and 30 mM) but not in cells treated with trigonelline alone (75 μM and 100 μM). However, the cells treated with high fructose along with...
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Posted by admin on Jan 31, 2019 in |
In this study, the synergistic effect of an extract of Azadirachta indica and Curcuma longa were investigated by the agar diffusion method. Different dilutions of the extract were prepared, and antifungal activity of the extracts of Azadirachta indica and Curcuma longa against Candida albicans was measured alone and in combination with fluconazole gel. The zone of inhibition of Azadirachta indica and Curcuma longa was found to be 9 mm to 14 mm and 8 mm to 13 mm respectively in the concentration range of 10-60 µL against Candida albicans. The fluconazole gel was prepared, and various evaluation parameters like; spreadability, pH, viscosity, and drug content were determined. Extracts of Azadirachta indica and Curcuma longa were taken in different strength (10-60 mg/ml) however the concentration of fluconazole gel was kept constant (1%) and zone of inhibition was determined. The maximum zone of inhibition was found with Azadirachta indica in the combination of 60mg/ml and Curcuma longa 20...
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Posted by admin on Jan 31, 2019 in |
The present study was designed to evaluate the muscle relaxant and locomotor activities of petroleum ether (PE), ethyl acetate (EA) and methanolic (MA) extracts of Malpighia emarginata (ME) and Holoptelia integrifolia (HI). The different concentrations were used to evaluate the toxicity in rats and revealed that they are nontoxic up to 2000 mg/kg. From the toxicity study, 200 and 400 mg/kg doses were selected for the pharmacological activities. Muscle relaxant and locomotor activities were done by Rotarod and Actophotometer respectively. The leaves and fruit extracts of PEME, EAME, and MAME with 200 and 400 mg/kg were showed a significant reduction in the time spent by the animals on the revolving rod when compared to the control (P<0.01, P<0.001) at 2 h. The MAHI of leaves and bark extracts (200 and 400 mg/kg) were more significant towards muscle relaxant effect (P<0.01, P<0.001) than PEHI and EAHI (P<0.05, P<0.01) when compared with control at 1 h. Treatment with both doses of leaves and fruit extracts PEME, EAME and MAME significantly (P<0.01,...
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Posted by admin on Jan 31, 2019 in |
Objective: The main aim of the present study is to investigate the activity of the anti-osteoporotic activity of Cucurbita pepo seed powder (CPSP) and low level laser beam (LLLT) on glucocorticoid induced osteoporosis in rat model. Materials and Methods: Animals were randomly divided into six groups containing six rats each (n=6). Group I normal control, Group II dexamethasone induced osteoporotic control (7 mg/kg b.w i.m.), Group III received dexamethasone + LLLT irradiation (180 sec/day daily), Group IV received dexamethasone + CPSP (100 mg/kg b.w p.o), Group V received dexamethasone + sodium alendronate (3 mg/kg b.w p.o), Group VI dexamethasone + CPSP + LLLT. The treatment is made for 8 weeks. On the end of 4th and 8th week body weight were recorded, behavioral parameters, locomotar activity using actophotometer and anxiety levels using elevated plus maze (EPM) was studied. Urine parameters (calcium, phosphorous, creatinine), the blood was withdrawn from retro-orbital plexus and the serum was used for the estimation of ALP, calcium, phosphorous, creatinine. At the end of the 8th...
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Posted by admin on Jan 31, 2019 in |
The desired target Compounds (4a-e), (5a-e) and (6a-e) were prepared in two steps. Reaction of 3-acetyl-6-bromo-2H-chromen-2-one (1) with 3-(Aryl)-1-phenyl-1H-pyrazole-4-carbaldehydes (2a-e) in presence of aqueous KOH solution and the reaction mixture stirred for 2 hours and then filtered, furnished the desired 6-bromo-3-((E)-3-(3-(Aryl)-1-phenyl- 1H-pyrazole-4-yl) acryloyl)- 2H-chromen-2- ones (3a-e) crystalline solids in 60-70% yield (Scheme-1). Further, compounds (3a-e) reaction with thiourea, urea and hydrazine hydrate in the presence of a base in EtOH yielded the compounds (4a-e), (5a-e) and (6a-e) in 50-85% yield. All the synthesized compounds have been characterized by spectral data’s, and they are screened for anti-microbial activity. Among synthesized compounds 3b, 3c, 3d, 5a, and 5d exhibited good activity against bacterial strains S. aureus, P. aeruginosa, and E. coli, respectively. Whereas other compounds showed poor to moderate activity against all bacterial strains and compounds 3a, 3b, 3d, 4c, 5d, and 5c exhibited good antifungal activity against A. niger, and A. flavus and remaining compounds exhibited moderate antifungal activity. Results were tabulated in Table...
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