Posted by admin on Jan 31, 2019 in |
The main objective of the present research was to develop mixed micelles using two biocompatible copolymers, D-α-tocopheryl polyethylene-glycol 1000 succinate (TPGS) and poloxamer 188 (P188) to improve the aqueous solubility and targeting efficacy of Simvastatin (SMV) against a variety of hormone-dependent cancers. A solvent evaporation technique prepared the plain/single copolymer micelles (SCMs) and mixed micelles (MMs). The prepared SCMs and MMs were characterized for critical micelle concentration (CMC), SMV content, particle size by dynamic light scattering (DLS), surface morphology by transmission electron microscopy (TEM), in-vitro SMV release and hemolysis. The SCMs and MMs showed mean particle size of 98 ± 5 nm and 129 ± 6 nm, respectively. SCMs showed SMV loading of 79.7 ± 5.6% while MMs exhibited improved SMV loading of 94.5 ± 6.5. The developed MMs system showed significantly lower CMC (3.5 fold less) than SCMs revealing their higher in-vivo stability. Moreover, SCMs and MMs exhibited zero order release profile, lower hemolytic behavior (<5% of hemolysis), when compared to plain SMV solution. The in-vitro cytotoxicity assay...
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Posted by admin on Jan 31, 2019 in |
A new derivative of Guar gum, incorporated with 1, 3, 4-thiadiazole nucleus was synthesized by using a new green and efficient synthetic approach. Guar gum is a biodegradable polymer having numerous applications in various industries. Thiadiazole compounds are well known in the therapeutic world due to their pharmacological importance. The newly synthesized derivative was characterized by IR, H1 NMR, and mass spectrometry. Its antioxidant activity was determined by hydrogen peroxide scavenging activity. Its antimicrobial activity was also...
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Posted by admin on Jan 31, 2019 in |
Analytical scientists of the pharmaceutical industry aimed at developing robust HPLC methods for analysis of generic drug products. This paper deals with HPLC method of analysis for estimation of % drug release of Ledipasvir and Sofosbuvir in Ledipasvir and Sofosbuvir tablets used for the treatment of chronic hepatitis C virus (HCV) infection. For analysis of dual combination drug product, HPLC method uses ‘Zorbax Eclipse Plus C18 100 × 4.6 mm, 3.5 µm’ HPLC column, a combination of buffer pH 3.0 and acetonitrile as mobile phase in gradient mode with UV detection at 260 nm. The method was validated and found to be precise, robust, accurate and linear (in range 9.05 to 54.3 µg/mL and 2.045 to 12.27 µg/mL of Sofosbuvir and Ledipasvir respectively). The method was also found to be specific for blank and placebo solutions. This ensures suitability of the method for simultaneous quantitative determination of % drug release of Ledipasvir and Sofosbuvir in a pharmaceutical formulation. In the pharmaceutical industry, this method can be used for routine...
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Posted by admin on Jan 31, 2019 in |
A recent surge of lifestyle disorders such as obesity, Type II Diabetes Mellitus and cardiovascular diseases, has led to a search of dietary changes that could be adopted into the daily lifestyle to prevent these lifestyle disorders. A nutraceutical is an upcoming option that has garnered a lot of attention and has warranted a lot of research. Green coffee beans are unroasted coffee beans which have high levels of antioxidants and caffeine thus making it a potential nutraceutical against Type II Diabetes Mellitus and obesity. In this study, water-soluble brews of caffeinated and decaffeinated green coffee beans were used to evaluate the ability of these extracts to alter the viability of muscle cells of Sus scrofadomesticus and also their effects on cortisol-induced (stress) hepatocytes by ex-vivo studies. The green coffee bean extracts were found to have low cytotoxicity. These extracts also highly controlled the release of glucose and regulated the gluconeogenic pathway by regulating glucose synthesis induced by cortisol in Sus scrofadomesticus hepatocytes when compared with the...
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Posted by admin on Jan 31, 2019 in |
Sericin is a globular protein obtained from degumming of silk cocoon in the silk reeling process. The sericin film was synthesized for the application of antibacterial wound dressing, but it has a low physical property, hence the synthetic polymer was used in the synthesis of highly stable biocompatible film. The PVA based sericin film was prepared by solvent casting method by the addition of synthetic polymers – polyvinyl alcohol (PVA) to give the mechanical stability and the glycerol was added as a plasticizer. The characterization of PVA based sericin film for its antibacterial properties and the swelling ratio was evaluated. The PVA based sericin film showed significant antibacterial activity for wound pathogenic bacteria such as E. coli, S. aureus, K. pneumoniae, P. aeruginosa, and Proteus species and the swelling ratio of the film was 70% which indicates that the sericin has high moisture holding capacity which improves the wound healing process. The sericin film was also proved that it was biocompatible for biomedical...
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