Posted by admin on Jan 31, 2019 in |
Aim of the study: The study was aimed to evaluate the antioxidant and anti-inflammatory activities of raw (ARCU) and distilled (ADCU) urine from an adult indigenous cow breed, Malnad gidda. Materials and Methods: Different radical scavenging models assessed antioxidant activity. Anti-inflammatory activity was assessed by carrageenan-induced rat paw edema method. The first group received saline and served as control. The second group received the standard drug Indomethacin (10 mg/kg b.w.), third (ARCU, 3 mL/kg b.w.), fourth (ARCU, 6 mL/kg b.w.), fifth (ADCU, 3 mL/kg b.w.) and sixth (ADCU, 6 mL/kg b.w.) groups served as treatment groups and received the urine samples.1 h later, paw edema was induced by injecting 0.1 mL of carrageenan (1% w/v) in saline solution into the sub-plantar region of the left hind paw of the rats. The paw volume was measured before the injection (basal volume) and after the injection of carrageenan at hourly intervals using Plethysmometer. Results: The ARCU and ADCU treated groups showed significant (P˂0.001) decrease in edema with a maximum inhibition of...
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Posted by admin on Jan 31, 2019 in |
Plants have been studied since ages for their therapeutic uses. In the earlier time, not much was known about the phytocompounds, but today the constituents of the different plants have been extracted and isolated for the use in the name of medicines or drugs. Flavonoid is one of the most characteristic classes’ of compounds in higher plants known to play several important roles including therapeutic uses in cancer treatment. The activation of oncogenes in the cancer cells can be regulated by the selective tyrosine kinases inhibitors, and therefore it can be considered as a promising approach for the targeted therapeutic development. Hence, in the present study, the authors tried to combine the knowledge of using plant Launaea procumbens in traditional medicine by analyzing and identifying the specific phytocompounds, especially flavonoids, and evaluating its anticancer potential. The scope of the present study is based on the aim to determine the possible use of plant Launaea procumbens in the field of therapeutics for cancer studies by performing in-silico docking analysis of...
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Posted by admin on Jan 31, 2019 in |
Fluoride becomes toxic at higher doses which leads to Fluorosis. In addition to dental and skeletal fluorosis, it also affects soft tissues including liver, heart, kidney, muscle, brain, etc. The aim of this study to examine the fluoride-induced oxidative stress and the protective role of Allium sativum ethanolic extract (ASEE) against underlying fluoride induced neurotoxic effects in the brain of rats. Three months old (250-280 wt.) Wistar rats were randomly categorized into 4 groups which were control (received normal tap water), sodium fluoride (NaF) (20 ppm fluoride through IP), NaF (20 ppm) + ASEE (120mg/kg body wt; through oral with plastic gavage), and ASEE. The doses are continued for 15 days, and on completion of treatments behavioral (Rotarod and Hot plate test), oxidative stress markers (LPO, SOD, CAT, GSH-Px) and Histological (Golgi Cox staining) observations were made. NaF treated rats showed significantly (p<0.01) decreased motor coordination, thermal pain response, SOD and Catalase activity. Whereas, LPO levels and GSH-Px activity increased in NaF treated rats. Moreover, they also showed a...
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Posted by admin on Jan 31, 2019 in |
Objective: To develop accurate, precise stability indicating a method for simultaneous estimation of Elbasvir and Grazoprevir in bulk and pharmaceutical dosage form. Materials and Methods: Simple, rapid, precise, sensitive and reproducible validated stability-indicating Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) method for the quantitative analysis of Elbasvir and Grazoprevir in the pharmaceutical dosage form. Chromatographic separation was carried out on waters Alliance-2695, by using Luna C18 (150 mm × 4.6 mm, 5 µm) column and the mobile phase containing OPA buffer (0.1%) and acetonitrile in the ratio of 50:50 v/v. The flow rate was 1.0 ml/min; detection was carried out at 258 nm using a photodiode array detector at ambient temperature. Results: The number of theoretical plates and tailing factor for Elbasvir and Grazoprevir were obtained to be NLT 2000 and should not more than 2 respectively. The linearity of the method was excellent over the concentration range 1.53-22.95 µg/ml and 3.05-45.75 µg/ml for Elbasvir and Grazoprevir respectively. The correlation coefficient was 0.999%. The relative standard deviation of peak areas of...
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Posted by admin on Jan 31, 2019 in |
Introduction: Autism spectrum disorders (ASD) are a group of mental problems that interfere with social interaction skills, and behavioral spectrum. ASDs are one of the most prevalent developmental disorders among children. The purpose of this study was to examine the mental health, quality of life, and happiness in mothers who had ASDs children and compare them with mothers having healthy children. Materials and Methods: This is a descriptive-analytic study. The population consisted of all mothers of children with autistic spectrum disorders (ASD) in Guilan province. The samples were determined through convenience sampling. We included 90 mothers whose children were identified with autism according to DSM5 criteria based on the clinical diagnosis of the child and adolescent psychiatrist and 90 mothers of children with no psychological complaint. The two groups were matched regarding demographic characteristics. The mothers of both groups completed a demographic questionnaire, the Symptom Checklist-90-R (SCL-90-R), the SF-36 Quality of Life Questionnaire, and the Oxford Happiness Questionnaire. The results were analyzed using descriptive statistics (i.e., mean, standard deviation)...
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