Posted by admin on Mar 31, 2019 in |
The present work involves the extraction of phytochemicals from the bark of the medicinal plant Hopea ponga, with various solvents and evaluation of their antimicrobial, anthelmintic and antioxidant activities by following standard methods. The antioxidant capacity of the plant extracts was measured by their ability to scavenge free radicals such as (a) DPPH (2,2-diphenyl-1-picrylhydrazyl), (b) ABTS (2,2’-azinobis-(3-ethylbenzenothiaziline-6-sulfonate), (c) FRAP (Ferric Reducing Antioxidant Power) (d) Hydrogen peroxide assay. The methanol extract exhibited, high antiradical activity against DPPH, ABTS with an IC50 value of 19.48 µg/ml, 25.22 µg/ml, respectively, higher content of phenols (195.11 ± 2.14 mg expressed as mg of Catechol equivalents/100 mg dried extract and highest anthelmintic activity among all tested extracts. The ethyl acetate extract exhibited high antiradical activity against hydrogen peroxide with an IC50 value of 38.00 µg/ml and exhibited significant activity against Streptococcus aureus with a maximum inhibition zone of 35.93 ± 0.12 mm for 1000 mg/ml. The present study suggests that the methanol extract may contain active compounds, which have effective antimicrobial, anthelmintic and antioxidant...
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Posted by admin on Mar 31, 2019 in |
Pyrazolines were prepared from the corresponding chalcones by using isonicotinylhydrazine as cyclizing agent. The synthesized compounds were characterized by spectral data and screened for antimicrobial activity. In-silico studies of physiochemical and drug-like properties revealed that these compounds have good bioavailability and drug-likeness properties. Compound PZ2 which exhibited good antimicrobial activity was incorporated into polymeric nanoparticles using PLGA polymer by solvent evaporation method. The prepared nanoparticles were characterized by particle size, entrapment efficiency (EE%), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and in-vitro release studies. Prepared nanoparticles were incorporated into different hydrogel systems, which were prepared by using Carbopol 934 and HPMC in different concentrations ratio 2:1, 1:1 & 1:2. Hydrogel formulations were evaluated for drug content, spreadability, viscosity and drug release studies. Hence, the studies confirmed that entrapment of pyrazolthe ine as antimicrobial drugs in nanoparticle systems has found to be an inventive alternative that improves therapeutic effectiveness and curtails undesirable side effects of the drugs. Further, incorporation of pyrazoline nanoparticles into hydrogel provides an additional benefit of...
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Posted by admin on Mar 31, 2019 in |
Potentilla anserina (Rosaceae) is a traditional medicinal plant in India, and it is available throughout the Northern hemisphere. This study was intended to evaluate the antinociceptive activity of methanolic extract of Potentilla anserina in acetic acid-induced writhing test in mice and Eddy’s Hot Plate method in Albino rats at the dose level of 75, 150 and 300 mg/kg p.o and the study was compared with the standard drug Indomethacin 10 mg/kg. The data were expressed as mean ± S.E.M. The statistical analysis was done using ANOVA followed by Dunnett‟s post hock test. The methanolic extract of Potentilla anserina showed significant antinociceptive activity in the acetic acid-induced writhing method the PAME (75, 150, 300 mg/kg, p.o) 1hr before a pain stimulus significantly reduced the nociceptive response. In the hot plate method, there was no significant difference in nociceptive behavior. The antinociceptive activity could be associated with the presence of several flavonoid-based bioactive compounds and their synergistic action with non-volatile bioactive...
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Posted by admin on Mar 31, 2019 in |
Background: The standard management of operable LABC is by initial modified radical mastectomy. Because of the high risk of metastasis, systemic therapy is involved as an additional integral part of treatment. The postmastectomy radiation is given to reduce the high risk (20-40%) of loco-regional relapse following adjuvant systemic therapy. The loco-regional control ranges from 85% to 90% for 10 years survival of 40% to 50% for operable stage III breast cancer treated through modified radical mastectomy, adjuvant systemic therapy, and post-mastectomy radiation. Objective: The Aim and objective of the study were to compare the loco-regional response in two arms for 4 weeks after completion of radiation therapy and at the end of six months of follow up and to compare acute and chronic toxicities in the two arms. Methods: In this study, patients with histopathologically established breast cancer were selected from out-patient Department of J. K. Cancer Institute Kanpur and were placed randomly into two different arms. Arm A delivered “40 Gy in 15 fractions over 3 weeks,” and...
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Posted by admin on Mar 31, 2019 in |
Incidence of fungal infections is increasing at an alarming rate, presenting an enormous challenge to healthcare professionals. Despite advances in preventive, diagnostic, and therapeutic interventions, the invasive fungal infection causes significant morbidity and mortality in immunocompromised patients. An effective alternative to conventional synthetic antifungal agents can be the use of medicines from natural sources for treatment of fungal infections. The present study is based on a comparative evaluation of preliminary phytochemical screening and in-vitro antimicrobial activity of petroleum ether and ethanolic extracts of Cassia tora seeds and leaves. Phytochemical screening revealed the presence of carbohydrates in all four extracts while anthraquinones were found in ethanolic extract of leaves and seeds. The in-vitro antimicrobial assay was performed by agar well diffusion method. Each of the extracts was screened for antimicrobial activity against Candida albicans, Staphylococcus aureus, and Escherichia coli. Ethanolic extract of leaves showed significant antimicrobial activity. The MIC of ethanolic extract of leaves was found to be 100 mg/ml, 75 mg/ml and 50 mg/ml against Candida albicans, Staphylococcus aureus, and...
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