Posted by admin on Jan 31, 2019 in |
Standardization is a process involving various chemical analysis methods based on pharmacological data involving general physical and microbiological analyzes aimed at the safety of a natural extract. To obtain the correct result, the standardization step is preceded by method validation. Quercetin can be obtained from the leaves of soursop (Annona muricata Linn.), which use for treatment fever, diarrhea, anti-convulsive, antifungal, antimicrobial, gout, itching, and flu. Determination of quercetin in soursop leaf extract using TLC-Densitometry must be validated by testing selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and range. Densitometry scanning of the plates silica gel 60 F254 directly at 265 nm was used for analysis of quercetin. The result of selectivity test was 1.53 (>1.5) with mobile phase toluene: ethyl acetate: formic acid (4: 3: 0.4; v/v/v), limit of detection (LOD = 0.018 µg), limit of quantification (LOQ = 0.060 µg), linearity y = 9387.3x – 767.39 (r = 0.9996 and Vxo = 1.9%), Coefficient of Variation (C.V.) of precision 1.906525% and the percent...
Read More
Posted by admin on Jan 31, 2019 in |
Hypercholesterolemia is one of the major conditions that are prevalent in today’s world, the significant causes being unhealthy food habits and lifestyle or abnormal genetic conditions. HMG CoA reductase is an important rate-limiting enzyme in the mevalonate pathway that synthesizes cholesterol. Biosynthesis of cholesterol in the liver can be reduced significantly by inhibiting HMG CoA reductase. Statins are the drugs used for the treatment of hypercholesterolemia. Due to the undesirable effects caused by synthetic statins, there is a need for the development of natural HMG CoA reductase inhibitors that are of plant origin. This study aimed to investigate the anti-inflammatory and HMG CoA reductase inhibitory effects of different hydro alcoholic plant extracts on the enzyme HMG CoA reductase activity obtained from Gallus gallus domesticus liver. Our study showed that the hydroalcoholic extract of Rosa centifolia exhibited the maximum inhibition of the enzyme under analysis with a ratio of 1.4117 and hence was subjected to solvent-solvent partition for the partial isolation of the inhibitory compounds. The result of this study...
Read More
Posted by admin on Jan 31, 2019 in |
Preformulation testing is the first step in the rational development of dosage forms of a drug substance. The preformulation investigations confirm that there are no significant barriers to the compound’s development as a marketed drug. The formulation scientist uses this information to develop an efficacious, stable and safe dosage form. The main objective of the present research work was to do preformulation study of piroxicam drug. Piroxicam (PC) is one of the most potent non-steroidal anti-inflammatory agents that also has anti-pyretic activity and has been used for the treatment of rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, tendinitis, bursitis, and for the pain that is not related to a musculoskeletal system and traumatic contusions. The Principal advantage of piroxicam is its long half-life, which permits the administration of a single daily dose. As the piroxicam is very effective drug, many researchers use this drug in their research. This research paper helps those people who want to use piroxicam drug for their...
Read More
Posted by admin on Jan 31, 2019 in |
Henna (Lawsonia inermis) is widely used cosmetically and medicinally. Literature survey reveals that the plant henna also has anticancer activity. Most of the anticancer activities of Lawsonia inermis plant are carried out using total leaves extract, and purified individual compounds also. The cytotoxicity profile of the extracts, as well as purified fractions, was determined by MTT assay on HeLa cell line. As the total ethanolic extract demonstrated growth inhibition in cancer cells, attempts were made to isolate the active compound from total ethanolic extract, with potent activity. Lawsone, 2-hydroxy-1, 4-naphthoquinone is the active constituent of Lawsonia inermis (Henna), L. alba, and other species of Lythraceae family. It is reported to posses various medicinal properties. The present study reports the anticancer activity of lawsone, naphthoquinone derivative isolated from the henna leaves, and its predictive conformation by spectral studies. Isolated lawsone was tested for the anticancer activity, which showed significant results....
Read More
Posted by admin on Jan 31, 2019 in |
In response to the spacious propagation of bacterial contamination stimulated by pathogens, the evolution of novel therapeutics with eminent antibacterial action is of avid concern. When the skin unity is ruptured accidentally or intentionally, its natural defenses will thus be vitiated, and topical medication may be prescribed. Topical medicaments are designed to either obliterate or inhibit the augmentation of pathogenic microorganism and can be consumed for both prophylaxes as well as treatment of superficial infection. Here is a novelize graphene-usnic acid conjugate was developed by way of a mere non-covalent process, and the loading and release demeanor of usnic acid on graphene were investigated. An effective loading of usnic acid on graphene prevailed at the initial usnic acid assiduousness of 300 mg/ml. The developed conjugate was embedded into biocompatible Eudragit polymers to formulate topically active microspheres. Formulated microspheres were characterized by FTIR, TGA, and graphene. Antibacterial activity was studied by the broth dilution method. Results disclosed that amalgamation of graphene and usnic acid had enhanced antibacterial activity against pathogen...
Read More
