Posted by admin on Mar 31, 2019 in |
Calotropis procera plant belongs to the Asclepiadaceae family. It is prescribed to treat bronchitis, asthma, cough, infections, cancer, ascites, intestinal worms, cutaneous diseases, eczema, leprosy and also aid to stimulate the immune system. The leaves of C. procera were investigated for their phytochemical and anti-microbial activity. Chemical screening of leaves ethanolic extract exhibited to contain active compounds like alkaloids, terpenoids, flavonoids, saponins, and reducing sugar. The antibacterial activity of C. procera leaves against four different bacteria revealed that the ethanolic extracts could kill only Staphylococcus aureus and Basillus subtilis, but more resisted by Pseudomonas sp., and Escherichia coli compare to the standard antibiotic streptomycin. GC-MS analysis of the ethanolic extracts indicated that the highest percentage in 100% ethanolic leaves extract belongs to camphene 6.22%, thebaine 7.59% dodecanoic acid 19.15%, and linolenic acid ethyl ester 14.87%, while the highest percentage in 70% ethanolic leaves extract belongs to hexa-hydro-farnesol 9.87%, gamolenic acid 12.71%, and linolenic acid ethyl ester 6.83%. This is the first phytochemical, and antibacterial study on C. procera leaves...
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Posted by admin on Mar 31, 2019 in |
The extensive study on O – methylated flavonoids reveals that substitution with methyl group enhances the radical scavenging behavior in certain compounds. Due to potential applications of C-glycosides in metabolic engineering, methylation in these flavonoids needs to be analyzed in both theoretical and experimental levels for their radical scavenging behavior. In this work naturally occurring C- glycosyl flavonoid apigenin 8-C-glucoside (vitexin) is theoretically simulated by substituting the C4′, C5 and C7 hydroxyl positions with methoxy unit and studied with the aid of density functional theory (DFT) for radical scavenging behavior. Structural stability is attained through B3LYP/6-311G(d,p) theory using Gaussian 03 package which provided the stable conformer for the studied compound without imaginary frequency. Structural activity is analyzed with the support of parameters like kinetic energy (temperature independent), HOMO-LUMO, molecular descriptors, Mulliken charge density analysis and compared with vitexin. Combined investigation of the parameters above revealed the superiority of phenyl hydroxyl vitexin over phenyl methoxy vitexin for radical scavenging activity unlike its O methylated...
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Posted by admin on Mar 31, 2019 in |
Aim: The present investigation was carried out by evaluating the effect of protocatechuic acid (PCA) on a high-fat diet (HFD) along with a low dose of alloxan-induced hyperglycemia in male Wistar rats. Methods: In the present study, administration of high fatty diet (HFD) for 45 days along with low dose of alloxan (80 mg/kg) in rats on 30 days produced significant high fatty diet in the, body weight, low density lipoproteins (LDL), triglycerides (TG) and blood glucose levels while decreases the high density lipoprotein (HDL) and serum insulin level. Protocatechuic acid (PCA) was started to administered at a dose of 50, 100, 200 mg/kg (orally) on day 45 of HFD administration and continue further 45 days. Glibenclamide was used as reference standard. Results: Results of PCA shows a significant decreased in body weight, blood glucose level after 45 days of treatment in diabetic animals. Result also indicated to increase in serum insulin level. The result of lipid profile indicated to normalize after 45 days treatment with PCA in diabetic...
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Posted by admin on Mar 31, 2019 in |
Biosurfactants are surface active, amphiphilic biochemical compounds containing both hydrophobic and hydrophilic moieties produced by microorganisms. They are produced by a variety of microorganisms mainly fungi, bacteria, and yeasts on their cell surface or extracellularly secreted. Biosurfactant mediated synthesis of nanoparticles has now emerged as a green alternative for enhancing both nanoparticles synthesis & stabilization. One of the modes of action is through adsorbing onto metallic nanoparticles, surface stabilizing the nanoparticles & preventing aggregation. Hence, biosurfactant mediated production of silver nanoparticles (AgNPs) was utilized in the present work. Biosurfactant was produced using Lactobacillus brevis and thus obtained biosurfactant was added to equal amounts of 1mM AgNO3 for production of AgNPs. The primary characterization of the AgNPs was done by visual observation. Further, AgNPs were characterized using UV-Vis Spectrophotometer which showed maximum absorption spectra at 420 nm, TEM revealed the size of AgNPs (18 nm). EDS was carried out to determine the presence of silver metal. Biosurfactant mediated AgNPs were assessed for the antimicrobial studies on MDR strains of bacteria...
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Posted by admin on Mar 31, 2019 in |
Enteroaggregative Escherichia coli (EAEC) strains are causal agents of persistent diarrhea in developing countries. Enteroaggregative Escherichia coli strains (EAEC) are an emerging type of diarrheagenic E. coli. We aimed to isolate, identify and characterize the E. coli pathotypes and study their antibiotic resistance in children under five with diarrhea in Kanpur region, India. Children under 5 years suffering from acute diarrhea and attending an outpatient clinic or admitted in Rama Hospital, Kanpur were enrolled for the study. Severe diarrhea is a public health problem and is responsible for morbidity and mortality, especially in developing countries. DEC was detected by multiplex PCR. Among 38 E. coli isolates, 12 samples contain Cvd432 gene of EAEC diarrheagenic E. coli. Most of the strains are resistant to ampicillin, Co-trimoxazole, gentamicin, azithromycin, imipenem. Ciprofloxacin, Norflex and chloramphenicol are relatively more sensitive. The high antimicrobial resistance observed in our study raises a broad discussion on the indiscriminate or improper use of antimicrobials, besides the risks of...
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