Posted by admin on Apr 30, 2019 in |
The synergistic activity among the monoterpene (S)-cis-Verbenol and the antibiotics Amoxicillin and Gentamicin were evaluated against a sensitive and a resistant strain of Staphylococcus aureus, is the last one also resistant to methicillin (MRSA). The combinations were evaluated by the checkerboard method. The combinations of both antibiotics with the terpene showed synergic effects for the MRSA strain, whereas for the sensitive strain one combination showed synergy with amoxicillin and additionally one combination showed the additive effect with Gentamicin. To confirm the observed results, the combination that had the lowest concentration of Amoxicillin and the monoterpene were assayed by the time-kill curve method. An even lower concentration of the terpene that did not show cytotoxicity against human cells was also assayed. The results obtained confirmed those of the checkerboard method and synergy was also observed for the non-cytotoxic concentration of (S)-cis-Verbenol analyzed. This opens interesting possibilities about the use of purely natural products in combination with antibiotics to treat infections produced by sensitive and resistant...
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Posted by admin on Apr 30, 2019 in |
Extracellular lipases are ubiquitous in occurrence and are essential for various applications such as organic synthesis, hydrolysis of fats and oils, modification of fats, flavor enhancement in food processing, resolution of racemic mixtures and chemical analysis. In the present study, an attempt was made to screen and characterize potent extracellular lipase producing bacteria from the pond water of Dakshina Kashi Kshetra, Antharagange, Vibhuthipura Village, Kolar Taluk, and Kolar District, Karnataka, India. Based on the ability to produce extracellular lipase the isolate was selected and identified through 16S rRNA sequencing as Acinetobacter...
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Posted by admin on Apr 30, 2019 in |
An accurate and precise HPLC method was developed and validated for simultaneous determination of Levocetirizine dihydrochloride, Phenylephrine hydrochloride and Paracetamol in syrup formulations. The separation between Levocetirizine dihydrochloride, Phenylephrine hydrochloride, and Paracetamol was achieved within 20 min using an L1 column of 100 × 4.6 mm, 3 µ dimension using gradient programme and detector wavelength 215 nm. The mobile phase solution A is a buffer solution of 0.015 Molar 1-octane sulphonic acid sodium salt adjusted pH 3.4 with orthophosphoric acid and mobile phase solution B is Acetonitrile. The Method was validated as per ICH guideline for parameters like Specificity, Precision, Accuracy, solution stability, filter compatibility, and robustness. Accuracy for Levocetirizine dihydrochloride, Phenylephrine hydrochloride, and Paracetamol lies between 97.0 to 103.0%. The proposed method can be used for quality control assay of Levocetirizine dihydrochloride, Phenylephrine hydrochloride and Paracetamol in...
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Posted by admin on Apr 30, 2019 in |
Different types of excipients were compared, in terms of their effect in (oral disintegrated tablets-ODTs) to optimize drug delivery and manufacturability. Therefore, the influence of excipients on the quality of ODTs was investigated by formulating and evaluate ODTs using an equal dose of water-soluble Baclofen (B) and poorly water-soluble Meloxicam (M) as model drugs. ODTs of both drugs were prepared using nine different co-process excipients for (B1-B9) and (M1-M9) formula (Pharmaburst®, Ludiflash®, F-melt®, Prosolv HD 90®, Prosolv SMCC 5O®, Prosolv ODT G2®, ProsolvEASYtab SP®, ProsolvEASYtab Nutra®, Lactose microfine), respectively by direct compression method. The prepared ODTs were evaluated for their: drug content, weight variation, thickness, disintegration time, wetting time, hardness, friability, and in-vitro dissolution. Both B-ODTs and M-ODTs showed no significant difference in the results of ODTs evaluation, by Using Design Expert 10 to select the best formulae of both drugs the best formulae were for poor water-soluble M9 (Lactose) > M3 (F-melt) > M6 (pro ODT) > M1 (Ph.brust) and for water-soluble: B4(Pro HD 90) > B3 (F-melt)...
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Posted by admin on Apr 30, 2019 in |
Oral extended release formulation with site-specific drug delivery formulations has been of greater importance in the field of drug delivery system to achieve adequate therapeutic results. The concept of novel gastro-retentive drug delivery system aroused due to site specificity behavior of the drug concerning drug absorption and solubility. The objective of the research work is to develop and evaluate gastro-retentive diltiazem hydrochloride modified pulisincap floating capsules. Diltiazem hydrochloride modified pulsincap floating capsules were formulated by filling the drug-polymer and diluents mixture in cross-linked hard gelatin capsules. Total of 15 formulations was developed and evaluated for pre capsule filling and post capsule filling parameters. Before development of formulation with different polymers, the cross-linked hard gelatin capsules were filled with only pure drug to carry out the diffusion studies of the drug through the cross-linked hard gelatin capsules among the developed, formulation containing carbopol 934p, i.e. F12 formulation was considered to be optimized based on its in-vitro drug release data and have shown the in-vitro drug release of 97.64 ± 3.45%...
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