Posted by admin on Apr 30, 2019 in |
A simple, sensitive and specific RP-HPLC method was developed and validated for the determination of Orotic acid in bulk and capsule dosage form. Chromatography was carried out on an Enable C18G (250 × 4.6 mm i.d.,5µ) column using filtered and degassed mixture of acetonitrile and methanol in the ratio of 60:40% v/v as mobile phase at a flow rate of 1 ml/min and effluent was monitored at 280 nm. The method was linear over the concentration range of 10 – 70 µg/ml with a correlation coefficient of 0.999. The retention time of the drug was 9.1 min. The proposed method was validated by determining sensitivity, accuracy, precision, robustness studies. The developed method was effectively applied to capsules of orotic acid, and the % assay of the drug was found to be 99.65%. The method is simple, accurate, precise and reproducible and hence can be applied for routine quality control analysis of orotic acid in pure and capsule dosage...
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Posted by admin on Apr 30, 2019 in |
Malaria is a foremost universal public health problem, especially in the African region and development of resistance to the older and new generation malaria therapy, has become a persistent health threat requiring urgent solutions. This study intended to assess the ability of human subjects to accurately reconstitute antimalarial dry suspensions according to the manufacturer’s instructions. Two hundred (200) dry powders for suspension were procured and distributed to 200 human subjects, and the volumes reconstituted were noted. Results showed that only 24 subjects (12%) were able to reconstitute the suspensions as required by the manufacturer, while ninety-seven subjects (48.5%) made reconstitutions above the required volume and 79 subjects (39.5%) reconstituted below the required volume. Error in reconstitution was as high as 76%. This study emphasizes the need for health caregivers; especially nurses and pharmacists to make the required reconstitutions before dispensing the medication and also give adequate counsel to patients on the appropriate way to make reconstitutions to avoid untoward effects due to over or...
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Posted by admin on Apr 30, 2019 in |
A series of condensed Imidazo [2, 1-b] [1,3] thiazole Chalcones have been synthesized by Claisen–Schmidt condensation reaction. Imidazo thiazole Chalcones are associated with immense biological activities. Novel Imidazo thiazole-based chalcones were synthesized using Imidazo thiazole-3-carboxaldehydes and various acetyl ketones in PEG-400. The structures of the compounds were characterized by IR, 1H NMR and screened for their in-vitro antioxidant (DPPH and SOD) activity. DPPH free radical scavenging activity was given by the compounds 7a-j at with (0.5 mmol/L in methanol). The compounds having substituent hydroxyl, fluoro, methoxy, ethoxy, and fluoro as well as benzimidazole and a ferrocenyl moiety in chalcone structure (7a-j) shows enhancement in antioxidant activity. Particularly in compounds like 7e, 7f, 7g, and 7h show the moderate percentage and 7c show the strongest percentage of inhibition mainly at (C=1mM). Almost all the compounds indicate moderate to good antioxidant activity compared to that of standard ascorbic acid percentage antioxidant activity (1 mm) by DPPH and SOD as 44.18 and 74.07 respectively. The resultant absorbance was recorded at 517 nm after 30 min incubation...
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Posted by admin on Apr 30, 2019 in |
A lectin curcin was isolated from mature seeds of Jatropha curcas L. Seeds were collected locally and purified by affinity chromatography on guar gum column. The lectin content was found to be 1.3 mg/ml. Curcin agglutinated B+ve human RBC type showing galactose and N-galactosylamine specificity. Galactose sugar was found to be the most potent inhibitor of curcin hemagglutinating activity (minimum inhibitory concentration (MIC) ≤ 50 mM). The lectin was a glycoprotein with a neutral carbohydrate content of 0.26µg/ml. The molecular mass of protein was found to be 28 kDa similar to previous reports of separation by SDS-PAGE. Curcin was stable at pH range from 4-10 while for temperature range it was stable from 10 ºC to 80 ºC for 30 min. Alignment with other lectin proteins by BLAST tool resulted into its similarity to 17 entries with 10 proteins with 100% query coverage but only RIP (Ribosome Inhibiting Protein domain) was visible in search results while carbohydrate binding domain was...
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Posted by admin on Apr 30, 2019 in |
Ethnic peoples from Purulia district of West Bengal in India use a lot of plants for their primary health care without studying phytochemical analysis and biological activities in detail. Main objectives of the present study are to evaluate the antibacterial and antioxidant activities of the whole plant extract of Curculigo orchioides using polar and nonpolar solvents. Total phenol content (TPC) was determined by Folin-Ciocalteu assay using Gallic acid (GA) as standard and total flavonoid content (TFC) by AlCl3 method using quercetin as standard. The antibacterial screening was carried out by agar well diffusion method. Methanol extracts exhibit higher phenol (82.93 ± 2.74 mg of GA E/gm of the sample), flavonoid (48.41 ± 1.94 mg of QE/gm of the sample) and alkaloid (5.56 ± 1.56%) content in comparison to other solvents. Similarly, methanol extracts show higher free radicals scavenging property and there was a positive correlation between IC50 values with total phenol and flavonoid content. A positive correlation exists in total phenol and flavonoid with antioxidant activities. The plant contains...
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