Posted by admin on Mar 31, 2019 in |
The study aims to develop an analytical method for the simultaneous estimation of Teneligliptin and Metformin using RP-UFLC. A simple, sensitive and accurate method was developed for Teneligliptin and Metformin using the chromatographic conditions of C18 Phenomenex Kinetex (250 mm × 4.6 mm i.e., 5 μm particle size) column in gradient elution mode with the mobile phase consisting of methanol, acetonitrile and potassium dihydrogen orthophosphate adjusted to pH 4.6 using orthophosphoric acid (40:20:40) with a flow rate of 1.0 mL/min, injection volume 10 µl and the eluent was detected at 250 nm using PDA and UV detector. The retention time of Teneligliptin and Metformin were found to be 5.2 min and 2.5 min respectively. The above method was validated concerning system suitability, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery) and robustness according to ICH guidelines. The linearity of the above methods was found to be 2-10 µg/mL for Teneligliptin and 25-125 µg/mL for Metformin. Hence, these methods can be used for routine analysis...
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Posted by admin on Mar 31, 2019 in |
Traditionally, mushrooms have importance in the diet. Since they possess both nutritional and medicinal value, research on mushrooms is the new area for developing lead molecules to treat cancer and other ailments. Ganoderma is a famous genus in the medicinally important mushrooms. Cancer is a fatal disease in which uncontrolled proliferation is the major crunch point to be considered. The current work is carried out to screen antimitotic activity of Ganoderma applanatum on legume seeds. Three legume seeds viz., Bengal gram, green gram, and fenugreek were selected for the study and the ability of the methanol extract to inhibit the germination was calculated. The results indicated that the extract is having a significant inhibition in the growth of the radicle of the seeds. The antimitotic activity can be attributed to the chemical constituents present in the extract. The phytochemical screening showed that the species is rich in various phytochemicals like alkaloids, glycosides, terpenoids, saponins, steroids, tannins, carbohydrates and proteins. These bioactive compounds, especially the tannins, terpenoids and steroids might...
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Posted by admin on Mar 31, 2019 in |
Artocarpus hirsutus Lam. is a common plant with a variety of folk medicinal value. The present study was conducted to evaluate the phytochemical and pharmacological properties of the ethanolic extract of Artocarpus hirsutus. The screening for various phytochemicals was conducted by using various standard procedures. Acute toxicity of the extract was determined by using OECD guidelines 423. The antidiabetic activity of the plant was evaluated by using the streptozotocin-induced diabetic model in Wistar albino rats. The histopathology of the pancreatic tissue was also performed. The antioxidant activity of the extract was also performed by using the liver homogenate of the animal. Statistical analysis was performed by using one way ANOVA followed by Dunnet’s test. The phytochemical screening of extract leads to the detection of various secondary metabolites like alkaloids, flavonoids, saponins, terpenoids, etc. No mortality was observed during acute toxicity studies up to the dose of 2000 mg/kg. The effect of the extract on parameters like body weight, fasting blood glucose level, SGOT, SGPT, ALP, bilirubin, total protein, urea,...
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Posted by admin on Mar 31, 2019 in |
The aim of this investigation was to design and optimize the Capecitabine niosomes derived from proniosomes using central composite design. Two independent variables viz., the molar ratio of drug to cholesterol (X1), surfactant loading (X2) and two dependent variables viz., the percentage drug entrapment (PDE) and mean volume diameter (MVD) were selected for the study. Proniosomes were prepared by a conventional slurry method and evaluated for the percentage drug entrapment (PDE) and mean volume diameter (MVD). The PDE dependent variables and the transformed values of independent variables were subjected to multiple regressions to establish a second order polynomial equation. Contour plots were constructed to elucidate the relationship between the independent and dependent variables further. From the computer optimization process and contour plots, predicted levels of independent variables X1, X2 (-0.77, -0.8 respectively), for an optimum response of PDE with constraints on MVD were determined. The polynomial equations and contour plots developed using central composite design allowed us to prepare niosomes derived from proniosomes with optimum...
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Posted by admin on Mar 31, 2019 in |
Gomphostemma Wall. ex Benth. belongs to the family Lamiaceae. The present study evaluated selected species of Gomphostemma endemic to the Western Ghats for their anti-inflammatory property. Traditionally, many species of Gomphostemma are used to treat malarial fever and inflammations caused by insect stings. Methanol extracts of Gomphostemma heyneanum var. heyneanum, G. heyneanum var. rottleri and G. eriocarpum were prepared and subjected to various in-vitro assays to examine their anti-inflammatory potential. Anti-inflammatory activity was initially screened with protein denaturation and proteinase inhibitory assays. Among the three extracts screened, G. heyneanum var. heyneanum showed significant activity comparable to the standard diclofenac sodium. Further, the effective extract of G. heyneanum var. heyneanum was evaluated for its effect on LPS induced RAW 264.7 cells. The effect of the extract on various inflammatory mediators like cyclooxygenase (COX), cellular nitrite and inducible nitric oxide synthase (iNOS) were analyzed. The extract inhibited cyclooxygenase and inducible nitric oxide synthase activity in a dose-dependent manner projecting the anti-inflammatory potential of the plant. The potent phytochemicals present in the...
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