Posted by admin on Mar 31, 2019 in |
In the recent development, worldwide consumption of alcohol has shot up in the past decade as a part of lavish and Gen next lifestyle changes. There have been innumerable instances of people consuming alcoholic beverages and drugs at the same time. Hence in our study, Diclofenac and Metformin were selected as representatives of highly prescribed and overused drugs. Hence, the present study was planned to design dissolution media and to study drug release of different brands of the same drugs using newly designed media. Various hydroalcoholic media were prepared. Optimization of new, chemically defined dissolution medium was done by comparison of release profiles of Metformin HCl and from Diclo IR 1, Diclo DR 2 and Diclo SR1, Diclo SR2 tablets of Diclofenac Na. The Metformin IR and Diclofenac IR tablets showed faster release in strong beer than mild beer and rum. It can be concluded that both the strength and volume play an essential role in the release pattern of IR formulations. The SR formulations of Metformin HCl and...
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Posted by admin on Mar 31, 2019 in |
An accurate and precise High-Performance Reversed-Phase Liquid Chromatographic and UV spectrophotometric methods were developed and validated for the quantitative determination of Cilnidipine, a novel generation Ca channel blocker in the pharmaceutical dosage form. Good quality chromatographic separation of Cilnidipine was carried out by using thermo scientific model C18 Column (4.6 mm i.d. × 250 mm, 5 µm particle size) (based on 99.999% ultra-high purity silica) using mobile phase consisting acetonitrile: methanol (50:50% v/v) at flow rate of 1.0 mL/min. The λmax of the Cilnidipine in methanol (as a solvent) was found to be 242 nm. The drug following linearity in the concentration range of 2-10 µg/mL with a correlation coefficient value of 0.9999 for both UV and HPLC methods. The regression equation for Cilnidipine UV method was found to be Y = 0.081x + 0.0017 and for HPLC method was Y = 25517x – 4755. LOD and LOQ were found as 0.15095 µg/mL and 0.45742 µg/mL for UV method and for HPLC method it was found to be 0.003...
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Posted by admin on Mar 31, 2019 in |
Linezolid has a better choice for the eradication of both community and hospital acquired methicillin-resistant Staphylococcus aureus (MRSA) infections, but its use is limited because of its complications. The study elucidated the efficacy and complications of prolonged therapy of linezolid in MRSA infected rats. The rats were rendered neutropenic by an intraperitoneal injection of cyclophosphamide injection given for 4 days and 5th day at a dose of 150 mg/kg and 100 mg/kg, respectively. This neutropenia was maintained for 5 days. The neutropenic rats were injected subcutaneously with 106 CFU/ml of MRSA. The rats were divided into 3 groups. Normal control, Infected, Infected animals treated with linezolid 50 mg/kg/twice/day for 14 days. On the 15th day, the blood and liver were collected for biochemical and histopathological examination. The MRSA was confirmed by PCR assay. The minimum inhibitory concentration of linezolid was 0.5-2 µg/ml. The decreased bacterial count (7.22 × 103 CFU / abscess), intestinal alkaline phosphatase (IAP), increased lactic acid, alteration in hematological parameters and liver damage were seen in...
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Posted by admin on Mar 31, 2019 in |
An essential pathological phenotype of chronic obstructive pulmonary disease is emphysema or lung tissue destruction. The study aims to assess docosahexaenoic acid (DHA), a precursor for specialized pro-resolving mediators, as a treatment of emphysema. Albino mice were challenged with porcine pancreatic elastase on day 1 and bacterial lipopolysaccharide on day 21 to induce emphysema. The pathological condition was then treated with docosahexaenoic acid for 7 consecutive days. The animals were treated with three experimental doses of docosahexaenoic acid (3, 10 and 30 mg/kg b.w.) and results were compared with another 2 groups receiving standard drugs, i.e., corticosteroid and bronchodilators. Histopathology, automated morphometry, bronchoalveolar lavage (BAL) and lung volume measurements were performed. The experiments showed that the 30 mg/kg dose of DHA has treatment effects similar to the standard drugs. The neutrophil is an inflammatory mediator, and the new treatment was able to reduce the neutrophil count in the bronchoalveolar lavage fluid. Lung volumes were reduced suggesting lesser hyperinflation. Histopathology confirms more inferior airway obstruction in the treatment groups. Even...
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Posted by admin on Feb 28, 2019 in |
In this study, we developed a cheap and precise analytic method to measure Etodolac concentration in bulk and pharmaceutical formula. The modified, updated method depends on the reaction between ferric ion and Etodolac to yield ferrous ion. Then the former ion reacted with potassium ferric cyanide to form colored compound has a maximum absorbance at 700 nm. The method was validated by calculating the precision and accuracy. The method follows Beer-Lamber law so that we used the range between 1-22.5 µg/mL and the correlation coefficient at 0.997. Accuracy and precision of the proposed method were estimated and shown average of recoveries between (96.67-99.0%) with precision represented by RSD % equal to 1.02. We also calculated the detection limit (LOD) and quantitative limit (LOQ), which respectively were 0.6 µg/mL and 1.2 µg/mL. The modified suggested method was used to measure Etodolac in pharmaceutical preparation without drug additives interference and shows a good agreement with the standard method. Therefore, it can be used for routine works for quality control for the...
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