Posted by admin on Dec 31, 2018 in |
Salbutamol is a bronchodilator, Guaifenesin is an expectorant and Ambroxol is a mucolytic. Combination of these drugs is used in the formulation of cough syrups. In the literature, there is no method reported for the simultaneous estimation of the drugs in oral liquid dosage form. Hence, the present work is aimed to develop reverse phase HPLC method for the simultaneous determination of Salbutamol sulfate (SAL), Guaifenesin (GUA) and Ambroxol hydrochloride (AMB) in oral liquid dosage form and validation of the developed method. The chromatographic separation of the drugs was achieved with the mobile phase system sodium dihydrogen phosphate buffer pH 3.0: acetonitrile: methanol in the ratio of 65:10:25 with the flow rate of 1 mL/min and injection volume 10 µL. An Inertsil C8-3 (250 × 4.6 mm, 5 µm) column was used, and the detection wavelength was 276 nm. This system produced sharp peaks with good resolution, minimum tailing and satisfactory retention times of SAL, GUA and AMB were found to be 3.157, 9.949 and 11.883 min respectively indicating...
Read More
Posted by admin on Dec 31, 2018 in |
A mathematical model optimizing bio-physico-chemical pre-treatment of steam exploded Piper nigrum, and Syzygium aromaticum lignocellulosic biomass waste was developed. The model was developed using, RSM, involving central composite face-centered design (CCD) with six parameters, three levels and 40 runs, at 95% confidence level. Six parameters optimized were quantity of each enzyme (a) Cellulase (ml); (b) Xylanase (ml) for enzymatic actions at 50 ºC; (c) Incubation time of 2-15 days for which substrate were dipped in various solvent systems; (d) Volume of solvent systems used; (e) Time of incubation at given steam pressure for steam explosion; and (f) Steam pressure 10-15 Psi. Cellulase and xylanase enzymes used were with activity (3.563 IU/mL), (33.32 IU/mL) respectively. ANOVA was applied for validation of the predicted model at 95% of confidence level. The Base 10 log transformation was selected from the comparative study, and the model predicted 13.79 µmoles/mL, the release of polyphenols at 2 days of incubation of substrate with 10 ml of the solvent system, under 15.0 psi steam explosion pressure...
Read More
Posted by admin on Dec 31, 2018 in |
This study aims at investigating the use of herbal medicine (F. vogelii) as a natural antioxidant to detoxify reproductive toxicity. Twenty-five (25) female Wistar rats used in this research were divided into five groups of five (5) rats per group. Group A served as negative control and received feed and water ad libitum. It also received formal saline. B and C served as the low (100 mg/kg) and high (300 mg/kg) dose extract groups. They received lead acetate for 14 days after the extract has been administered for first 7 days. Groups D served as the positive control and received only lead acetate 14 days too while E received only the extract for 14 days. The experiment lasted for 28 days including an acclimatization period of 7 days. The positive control group showed a significant increase in SOD activity at P ≤ 0.01 while that of group E rats had ovarian SOD closer to group A. Group B and C showed reduced SOD level as well as significant weight...
Read More
Posted by admin on Dec 31, 2018 in |
Currently, nutrition and health linkages focused on the emerging strategy of diet based regimen to combat various physiological threats including cardiovascular disorders, oxidative stress, diabetes mellitus, etc. Berry / Fruit part of Diospyros kaki Linn. was extracted using petroleum ether, chloroform, ethanol consecutively and the obtained extracts were screened for hepatoprotective activity using CCl4 induced liver damage model. The activity was assessed by comparing the serum enzyme levels such as serum glutamate pyruvate transaminase, serum glutamate oxalate transaminase, total bilirubin, alkaline phosphatase of fruit extracts treated group with carbon tetrachloride treated animals and results showed dose-dependent activity, ethanolic extract treated group showed highly significant activity, whereas chloroform extract treated group has shown the significant action but less compare to ethanolic extract, petroleum ether treated group showed moderate action and petroleum ether extract at a dose of 100 mg/kg b.w showed least significant action. The results were further supported by histopathological...
Read More
Posted by admin on Dec 31, 2018 in |
Diabetes is a non-communicable disease characterized by increased blood glucose. As regular monitoring of glucose and glycated hemoglobin (HbA1c) levels in diabetic individual’s blood causes psychological stress, development of a non-invasive technique to quantify glucose and HbA1c are essential. This study was carried out to compare and correlate the blood and salivary glucose and blood HbA1c levels in diabetic individuals. Results showed that mean blood and saliva glucose levels were higher in the uncontrolled diabetic group than controlled diabetic and control groups. Blood HbA1c level was also elevated in uncontrolled type-2 diabetes (T2D) group, followed by controlled diabetic and control groups. A significant difference was noticed between the blood and saliva glucose levels of control and uncontrolled T2D groups. Further, the difference in serum HbA1C levels between control, controlled T2D, and uncontrolled T2D groups was also significant. Taken together, these results explicated the use of saliva as a non-invasive, painless technique in the management of T2D...
Read More
