Posted by admin on Jan 31, 2019 in |
The aim of the present study was to prepare solid lipid nanoparticles (SLNs) of azathioprine to increase its permeability and to develop azathioprine loaded solid lipid nanoparticles (AZA SLNs) based transdermal film for topical administration with the aim of reducing systemic and gastro-intestinal side effects of the drug and achieve passive targeting of drug to the joint due to enhanced permeability and retention (EPR) effect. Azathioprine SLNs were prepared by hot homogenization technique and optimized by 32 factorial design to evaluate the impact of the formulation variables on the dependent variables. Optimized formulation was evaluated for various in-vitro attributes and then incorporated into a transdermal film by a solvent casting method, which was evaluated for various technological properties and ex-vivo permeability study. Size of the optimized SLNs was found to be 113.3 ± 5.09 nm with 0.308 ± 0.008, polydispersity index (PDI) and encapsulation efficiency of 80.28 ± 1.29%. The in-vitro release studies of optimized formulation showed sustained release up to 24 h possessing biphasic pattern with a burst...
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Posted by admin on Jan 31, 2019 in |
Lup-20(29)-en-3-ol, known as Lupeol is one of the most extensively studied triterpene compounds for their diverse biological properties. Natural triterpenoids gain pre-eminence in cancer research owing to their effects in regulating transcription factors and pathways associated with cell proliferation. The objective of the present study was to evaluate the anticancer activity of Lupeol on human adenocarcinomic human cell lines A549, and expression of anti-apoptotic genes. A549 cell line used in the present study as an in-vitro model of non-small cell lung cancer provides an insight into the apoptotic and anti-metastatic potential of Lupeol. Antiproliferative effects and apoptotic potential was measured using 3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and Acridine orange/Ethidium bromide staining method respectively. Invasion assay was used to mimic an experimental system to determine metastatic activity and effect of Lupeol on apoptosis-related genes was validated with RT-PCR. The results revealed that Lupeol decreased the cell viability in a dose-dependent manner with an LD50 value of 62.53 mg.mL-1. Presence of apoptotic bodies with membrane blebbing suggests the apoptotic...
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Posted by admin on Jan 31, 2019 in |
Siddha system of medicine is a traditional medicinal system being followed mainly in South India. It is better known as one among the AYUSH medicinal system. The present study aims to standardize the Siddha poly-herbal formulation Panchabakiya chooranam (PBC), which is mentioned in the Siddha classical text Anuboga vaidya Brahma ragasyam indicated for stomach burn, burning sensation in anus and diarrhea. Accordingly, PBC was studied through Inductively Coupled Plasma Optical Emission Spectrometric analysis (ICP-OES) and Fourier Transform Infrared Spectroscopy (FTIR) techniques to identify the metals and functional groups present in it. Results showed that the sample contains elements such as Calcium, Iron, Sodium, Potassium, and Phosphorous. Among the detected elements Phosphorus was present in a maximum concentration, and iron was in lowest concentration. Moreover, the toxic metals namely, Mercury, Arsenic, Cadmium, Nickel, and Lead were in Below Detectable Level, which ensures that the drug is safe to consume. Besides, functional groups such as Alcohols, alkanes, amides are identified using IR...
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Posted by admin on Jan 31, 2019 in |
The aim of the present investigation was to enhance the dissolution of poorly water-soluble drug Ramipril (BCS – II) drug through Liquisolid compact. The liquisolid compacts were prepared with a varied quantity of liquid and powder ingredients using microcrystalline cellulose as a carrier, Aerosil 200 as a coating material, polyethylene glycol 400, propylene glycol and tween-80 as liquid vehicles, HPMC as additives to increase loading capacity. The required quantities of ingredients for the compressible admixture were calculated using various mathematical models. The in-vitro release characteristics of different dissolution conditions were performed. All formulated systems were assessed for precompression parameters like flow properties, FTIR analysis, X-ray diffraction (XRD), differential scanning calorimetry (DSC), and post-compression parameters like content uniformity, weight variation, hardness, friability, disintegration test, wetting time and in-vitro dissolution studies. As liquisolid compacts demonstrated significantly higher drug release rates, thereby indicating a promising strategy in improving the dissolution of poorly water-soluble drugs and formulating immediate release solid dosage...
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Posted by admin on Jan 31, 2019 in |
The purpose of the research was to develop and evaluate metronidazole loaded HPMC K15M mucoadhesive microcapsules for sustained drug release at the gastric mucosa. Metronidazole mucoadhesive microcapsules were formulated by ion gelation technique using 32 factorial designs. A 32 full factorial designs were used to derive a statistical equation, ANOVA analysis, contour plots, and 3D response surface plots. Different polymer ratios of HPMC K15M and sodium alginate were used to formulate nine formulations (F1 to F9) of HPMC K15M loaded mucoadhesive microcapsules of metronidazole. In-vitro drug release and mucoadhesion were carried out by USP29 type-II tablet dissolution test apparatus and disintegration tester using goat stomach mucosa. The formulation was characterized by determining possible drug-polymer interaction using FT- IR, the percentage of yield, particle size, the percentage of entrapment efficiency, swelling index, the percentage of mucoadhesion and percentage of drug release. FT-IR spectroscopy result shows the interaction between the drug and polymers combined. The optimized formulations F9 exhibited high drug entrapment efficiency of 92.07 ± 0.02%, particle size of 852.46...
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