Posted by admin on Jun 30, 2024 in |
The present study has been designed to investigate the effect of SU6656, a selective inhibitor of src kinase and BAY 11-7085, a selective nuclear factor kappa B inhibitor, as potential target in a mouse model of spontaneous alcohol dependence induced withdrawal syndrome. Our experimental protocol consisted of administration of Alcohol (2 g/kg, 10%, v/v, oral), once daily for 7 days. Assessment of behavioral parameters and exploratory parameters was done on 7 day after 8 hr. of the last ethanol administration for a period of 120 minutes (90 minutes for behaviour and exploratory parameters and 30 minutes for depression and hyper responsiveness parameter). Ethanol withdrawal behaviors were hyper excitability (seizures) and this hyper excitability was behaviorally present in terms of super sensitivity to sub convulsive dose of PTZ (30 mg/kg, i.p) a convulsant. Withdrawal syndrome was quantitated in terms of a composite withdrawal severity score, exploratory behavior which was confirmed by, reflective of depression like behavior by force swim test, hyperalgesia by tail flick test. SU-6656 (1. 5 & 10...
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Posted by admin on Jun 30, 2024 in |
Plant extracts are in use in the form of decoctions and water infusions in Ayurvedic systems of medicine since ancient times. It is generally believed that particles finer in size, produce more amount of extract yield. However, there appears to be no uniformity in using powders of any particular particle size for extraction. Coarse and fine powders of different particle sizes have been observed used, by researchers and manufacturers for extraction. The object of the present study is to study the effect of the coarse and fine particles on percentage of extract yield in plants. The study is conducted on 27 medicinal plants frequently used in Ayurvedic medicine. Soxhlet extraction method using water as solvent is followed in this study. The study concludes that certain group of plants yield higher extract from coarse powders while some produce higher extract yield from fine powders. Whereas, no difference in yield of the coarse and fine powders is observed in some plants. Ayurvedic texts classify the plant material in accordance with material...
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Posted by admin on Jun 30, 2024 in |
Cyamopsis tetragonoloba belongs to the family Fabaceae and has notable medicinal properties as it is used as a digestive aid. Guar Gum, which is a high molecular weight carbohydrate with multiple industrial applications, is also obtained from this plant. Methanolic extracts of C. tetragonoloba were obtained through reflux extraction to perform further experiments. Various experimental tests were carried out to understand the physicochemical, antimicrobial, and fluorescence properties of C. tetragonoloba. HPTLC and phytochemical profiles were also obtained. Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, saponins, steroids and triterpenes. Microscopy of the sample powder showed the presence of macrosclereids and thickened xylem vessels. The maximum antimicrobial activity of C. tetragonoloba was seen at a sample concentration of 10 µL where the activity was more than standard against Escherichia coli and Aspergillus niger. HPTLC profiling showed 7 bands each in 5 lanes of increasing concentration and a pattern unique to C....
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Posted by admin on Jun 30, 2024 in |
Symmetric peak and effective peak resolution in chromatography were achieved by adjusting the mobile phase’s pH and amount of organic Phase. The retention time of 2.127 and 4.145 minutes was observed after the elution of Bortezomib and Selinexor respectively. To accurately measure both Selinexor and Bortezomib at the same time, an approach was developed with an optimal wavelength of 236nm. Selinexor and Bortezomib were successfully separated by chromatography using a mobile phase of Acetonitrile: 0.1% Formic acid (30:70, v/v) and Luna Phenyl Hexyl (250×4.6mm, 5μ) column running at 1.0 ml/min. When looking at the linearity range, statistics correlating 0.999 indicates a very excellent correlation. For intermediate precision intervals, the relative standard deviation of Selinexor and Bortezomib, respectively, was 0.47 and 0.53, indicating the reproducibility of the analytical approach. The suggested analytical approach has a low LOD, which means that it is also extremely sensitive. This indicates that the suggested approach has sample accuracy by ICH requirements, as 99.9 and 100.2 percent of the spiking Selinexor and Bortezomib, respectively, were...
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Posted by admin on Jun 30, 2024 in |
A new, simple, and sensitive differential scanning calorimetry and infrared spectrophotometric technique was developed to estimate ethambutol hydrochloride in tablet dosage form. In the first method the concentration range of ethambutol hydrochloride was discovered to be 0.5 mg to 2.0 mg using differential scanning calorimetry (DSC), with the peak region of an exothermic thermogram at 75.8 °C and a continuous heating rate of 5 °K min-1. Second method which involves the measurement of ethambutol hydrochloride by Infra Red (IR) spectroscopy, the concentration range was determined to be 1.0 mg to 2.5 mg. The correlation coefficients for these techniques were determined to be 0.999 and 0.9919 for both DSC and IR methods respectively. The analytical results were statistically validated by the ANOVA technique and recovery trials. The recovery trials show no influence from other chemicals in the formulation. As a result, these two procedures are easy, precise, accurate and specific, and they might be employed for regular...
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