Posted by admin on Feb 28, 2019 in |
Development of quality assessment parameters for natural products is a prominence necessity to justify their acceptability and activity. Establishment of authentic and reliable analytical methods which profile the quantitative phytochemical composition of marker constituents in multicomponent composition like extract is a challenging task. A simple, rapid, precise, and reliable HPLC method was developed for the separation and estimation of ellagic acid from Epilobium angustifolium (Canadian willow herb) extract. The estimation was carried out using Sunfire C18 column, 5μ (4.6 × 250 mm); mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile; the flow rate of 1 mL/min and ultraviolet detection at 280 nm with a properly resolved having run time of 35 min. The method was validated as a final verification of method development concerning precision, linearity, accuracy, ruggedness, and robustness. The correlation coefficient (r2) > 0.999, a method is considered to be linear as the correlation coefficient was found to be within acceptance criteria. The % RSD of peak area response due to ellagic acid in five replicate injections of standard...
Read More
Posted by admin on Feb 28, 2019 in |
In this study, radioprotection against radiation-induced hepatic damage in Swiss albino mice has been observed by Aloe vera. When individuals are exposed, the radiation energy is absorbed by the biological systems, which causes radiolysis of tissue water and generates free radicals. Animals were given Aloe vera leaf extract orally 1000 mg/kg body weight/day for 15 consecutive days before radiation exposure 0.5, 3 and 5.5 Gy gamma radiation. Mice were autopsied at day ¼, 1, 3, 5, 10 and 20 after irradiation to evaluate the radio modulator effect in terms of protein, cholesterol, and glycogen. In control set animals radiation treatment showed an increase of protein, glycogen, and cholesterol, however, the animals of experimental group, i.e., Aloe vera and radiation combined group showed a significant decrease in protein, glycogen and cholesterol but values remained below normal. It can be concluded that Aloe vera modulate the radiation-induced biochemical alterations in Swiss albino...
Read More
Posted by admin on Feb 28, 2019 in |
Euphorbia neriifolia and Euphorbia hirta have been traditionally used for relieving anxiety and other nervous disorders. The present study was designed to evaluate and compare the antianxiety activity of E. nerrifolia leaves and E. hirta aerial parts using elevated plus maze model. Petroleum ether (60-80°C), chloroform, ethanol and aqueous extracts of both the plants were prepared and evaluated for antianxiety activity. Chloroform extract of E. neriifolia and ethanol extract of E. hirta showed significant antianxiety activity at the dose of 200 and 400 mg/kg, respectively, comparable to that of diazepam (2 mg/kg). The study was extended to develop the TLC fingerprint profile of the two plants using CAMAG HPTLC system, and the relative percentage of the separated constituents was calculated from the area of peaks. Further, phytochemical screening of the bioactive chloroform extract of E. neriifolia showed the presence of flavonoids, steroids/terpenoids, phenols, and tannins while the bioactive ethanol extract of E. hirta tested positive for flavonoids and...
Read More
Posted by admin on Feb 28, 2019 in |
Background: Cutaneous adverse drug reactions (CADRs), having a wide range in terms of its spectrum, are an important cause of morbidity and mortality. They comprise 2-3% of all ADRs. This study was conducted to analyze various parameters of CADRs at our setup. Methodology: This prospective, observational study was carried out for 1 year at our setup after ethical approval. Those diagnosed as CADRs by the Dermatologist were included. Data was collected in suspected ADR reporting form and analyzed for various parameters like suspected drug and drug class for CADRs, spectrum as well as its type, severity, preventability, causality assessment, seriousness, and outcome. The lag period for the development of CADRs was also analyzed. This data collection was also uploaded via Vigiflow under Pharmacovigilance Programme of India (PvPI). Results: Total of 125 cases were recorded with 134 CADRs. These 134 cases included a wide range comprising of 27.61% rashes to 5.97% Stevens-Johnson syndrome (SJS). For these CADRs, most common suspected drug classes were anti-infectives (34.32%) followed by NSAIDs (29.10%) whereas...
Read More
Posted by admin on Feb 28, 2019 in |
An alternative energy sources using the sweet orange (Citrus sinensis (L.) waste as the feedstock was investigated; this is an approach to environmental protection. The extraction of pectin from sweet orange peel waste (pith) and the production of ethanol from the resultant liquid pectin with the aid of Escherichia coli (E. coli) and Saccharomyces cerevisiae (yeast) were carried out. Dried pith was separated using various particle sizes ranging from 0.075, 0.5, 1.0 and 5 mm. It was observed that pith with a particle size of 1.0 mm produced a larger volume of pectin while the pith with a particle size of 0.075 mm produced the least volume of pectin. 1,770 ml pectin was obtained from 802 g of pith; this shows that citrus fruit (especially orange) contains a high amount of pectin. E. coli (bacteria), yeast (fungus) and a mixture of both were added to the produced pectin which was fermented to ethanol. It was however noticed that sample pectin + E. coli + yeast, and sample pectin +...
Read More
