Posted by admin on Dec 31, 2018 in |
The purpose of the present study describes the preparation of pH based in-situ gel formulations were applied for the harmful bacterial infections by using carbopol 934P and HPMC derivatives. The simple procedure was adopted for preparing these formulations. To eliminate or reduced adverse systemic effects produce from bacterial conjunctivitis, topical route of administration, such as eye drops was preferred for this type of infections and it should be installed into the eye’s cornea. These eye drops are more effective to provide therapeutic index and also to achieve good bioavailability in the eye. Forthcoming, the developed in-situ gel formulations were characterized by gelling capacity, pH, clarity, appearance, viscosity determination, surface tension, drug content, in-vitro drug release, HET-CAM study, permeation study, and in-vivo ocular irritation study. The optimized formulation (F2) was set up to be non-irritant and well tolerable from the results both in-vitro HET-CAM study and ocular irritation study. The developments in-situ gel formulation forms good gelling capacity, clear/translucent in appearance, after administration in the eye. The developed in-situ gel...
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Posted by admin on Dec 31, 2018 in |
Neuroprotection is utmost necessary due to the devastating neuronal injuries, including neurodegenerative disorders. N. sativa L. is called the Miracle herb due to its wide usage in traditional systems of medicine, especially Arabic. This plant has been studied for its therapeutic potential and found to possess a wide spectrum of activities, including anticancer and antioxidant properties. The present work is designed to investigate the acetylcholinesterase inhibitory and cytotoxic potential of various grades of black cumin oil for its neuroprotective activity. Thymoquinone is the most pharmacologically active ingredient found in black cumin oil. The various grades of black cumin containing 5%, 2% and 0.6% thymoquinone at different dose levels is used throughout this work. Acetylcholinesterase inhibitory is performed by using Ellman’s method, and the cytotoxic potential is evaluated by using Sulforhodamine B assay employing IMR-32, U373-MG, and SK-N-SH cell lines. Black cumin oil containing 5% thymoquinone showed a dose-dependent increase in the acetylcholinesterase inhibition (64.47% inhibition at the dose of 20 µg/ml) which is similar to that of the standard...
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Posted by admin on Dec 31, 2018 in |
Nanoparticles speak to one of the appealing choices in the compelling treatment of tumor chemo-treatment. In the present work, definition, and improvement of a novel Cetuximab (MTX)- stacked biodegradable nanoparticles utilizing poly(D, L-lactide-co-glycolide) (PLGA) was done. Nanoparticles stacked with anticancer operators can effectively build sedate focus in malignancy tissues and furthermore act at the cell level, improving antitumor viability. These medication conveyance frameworks enhance bioavailability by improving fluid solvency, expanding protection time in the body and focusing on medication to a particular area in the body. The arranged nanoparticles were assessed for physicochemical properties, for example, molecule measure, zeta potential, discharge thinks about, and so forth. Molecule size of the upgraded definition was < 200 nm. Our essential outcomes exhibit that the created Cetuximab-stacked PLGA nanoparticles are discharging the medication for the delayed timeframe. The goal behind the work is to contemplate the impact of definition factors on the molecule estimate, sedate exemplification and % aggregate medication arrival of...
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Posted by admin on Dec 31, 2018 in |
In this study, we investigated anti-cancer and antimicrobial activity of Aegle marmelos leaf extracts and their chemical profile characterized by gas chromatography coupled mass spectrometry (GC-MS). A. marmelos leaves were extracted with acetone, methanol, ethanol, and chloroform. Presence of phenolic compounds was identified in these extracts by qualitative analysis. All the extracts were subjected for anti-bacterial activity against the different strains of bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Bacillus ariyabattai, Bacillus megaterium, Pseudomonas putida, Klebsiella pneumonia, Serratia marcescens, and Escherichia coli). It is noteworthy that acetone extract elicited maximum growth inhibition on Serratia marcescens. Based on profound anti bacterial activity, acetone and methanol extract of A. marmelos were checked for cytotoxicity against MDA-MB-231, HEp-2 and vero cells. MDA-MB-231 cells were more sensitive to acetone extract of A. marmelos with an IC50 value of 79.62 µg/ml where as HEp-2 cells are more sensitive to methanol extract of A. marmelos with an IC50 value of 47.08 µg/ml. Vero cells withstand 24 h treatment of both extract, and it is evidenced...
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Posted by admin on Dec 31, 2018 in |
Aztreonam, a synthetic beta-lactam antibiotic is widely used for the treatment of infections in lungs, meninges, bladder, etc. Its potential use in the treatment of pneumonia and cystic fibrosis has been established. Recently FDA has approved Cayston® for inhalation therapy of Aztreonam in lungs infection. Many studies have been reported on the quantitative determination of Aztreonam by UV spectrophotometric and HPLC method. The present study focuses on the quantitative spectrophotometric determination of Aztreonam in two simulated lungs fluid namely artificial lysosomal fluid (ALF) and gamble solution. The λmax was found at a wavelength of 293 nm for both the solutions. Further, the method developed was validated for its linearity, precision by interday and within day study, accuracy, specificity, robustness and determination of limit of quantification and limit of detection in both the solutions. The linearity demonstrated a correlation coefficient of 0.9995 and 0.9999 in ALF and gamble solution respectively. The LOD was found to be 0.38 µg/ml and 0.18 µg/ml for ALF and gamble solution respectively. The LOQ was...
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