Posted by admin on Apr 30, 2024 in |
A simple, rapid, precise, sensitive and reproducible Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Tepotinib in presence of its impurities (1&2) in pharmaceutical dosage form. Chromatographic separation of Tepotinib and its known impurities were achieved on Waters Allianace-e 2695 by using Inertsil ODS C18 250×4.6mm 5µ column and the mobile phase containing (0.01M) KH2PO4 PH-2.5&Acetonitrile in the ratio of 50:50% v/v. The flow rate was 1.0ml/min; detection was carried at 263nm using a photodiode array detector at ambient temperature. The calibration range was 10-60µg/ml for Tepotinib, 2.5-15µg/ml for Imp-1& 3.75-22.5µg/ml for Imp-2. %Relative standard deviation of peak areas of all the measurements less than 2%. Recovery was obtained between 98-102%. Stability studies were also performed with known impurities the drug showed maximum degradation in alkaline stress condition. The proposed method was validated according to ICH guidelines. The results obtained were within the acceptable limits. The proposed method was found to be simple, economical, precise, accurate & robust for quantitative analysis...
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Posted by admin on Apr 30, 2024 in |
Endocrine-disrupting chemicals are environmental pollutants that have been associated with a wide range of diseases, including breast cancer, the most prevalent cancer in women worldwide, according to the WHO. Among the different cancers affecting the female population, breast cancer has the highest incidence and mortality rate. The incidence of breast cancer is 100 times higher in women than in men. The incidence of breast cancer is alarmingly high, so the need for a novel approach with fewer side effects is currently needed to improve the quality of life of the patients. Catharanthus roseus is a plant species belonging to the Apocynaceae family that is used worldwide in phytotherapy. The indolic alkaloids (vincristine and vinblastine) isolated from C. roseus are approved and used in clinical trials. The interest of the study lies in the comparison of indole alkaloids (vincristine and vinblastine) against breast cancer targets through in-silico studies. The work of the study lies upon downloading target and ligand. The targets that are associated with breast cancer were downloaded from...
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Posted by admin on Apr 30, 2024 in |
Periodontitis is a widespread oral gum infection having multiple antibiotics for the treatment, but large oral doses and the emergence of antibiotic resistance constrain their usage. The goal of this study was to develop and characterize buccal mucoadhesive patches made of clindamycin and polymers for the treatment of Periodontitis. Patches were developed by solvent casting method using Clindamycin Hydrochloride, an herbal constituent of Curcumin, and polymers like Polyvinyl Alcohol, and Hydroxy Ethyl Cellulose. Eight trial patches were developed by keeping the drug ratio constant and varying the concentration of different polymers. The optimized patch concentration was chosen to formulate the buccal mucoadhesive patch to treat periodontitis. Optimization was conducted by Design Expert Software (DoE) using 23 full factorial designs by taking three independent variables as factors and two dependent variables in-vitro Drug release (Y1) and Drug content (Y2). Prepared buccal mucoadhesive patches were flexible and showed the highest efficacy in the inhibition of oral microorganism species like Porphyromonas, Streptococcus, Actinomyces, and Candida albicans. The optimized patch was analyzed for...
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Posted by admin on Apr 30, 2024 in |
Punica granatum L. is the reddish colour fruit, commonly known as Pomegranate. It is known to have many health and therapeutic benefits which can be used as agent. The pomegranate juice is also used to prepare wine. The present study was carried out to determine the optimum fermentation parameters of pomegranate wine preparation. The antimicrobial and antioxidant activity of various wine preparations were also evaluated for their health benefits. The wines were prepared using various brix values under both aerobic and anaerobic conditions. The antimicrobial and antioxidant activity which was carried out using the agar cup method and DPPH assay respectively. The best wine was found to have 15.3% alcohol prepared with 0°B and aerobic conditions in 8 days. The antimicrobial and antioxidant activity of the wines prepared were higher than the fresh P. granatum juice. Therefore, we suggest that pomegranate wine is a better option of fruit juice storage having better antimicrobial and antioxidant...
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Posted by admin on Apr 30, 2024 in |
Chenopodium album L., Chenopodium murale L., and Chenopodium giganteum L. are annual herbs with ethno-medicinal value. The present study investigates the primary and secondary metabolites quantification of Chenopodium species. The quantitative estimation of bioactive molecules from the root, stem, and leaves showed the presence of proteins, carbohydrates, starch, phenols, and flavonoids. However, these metabolites vary in root, stem, and leaves. The result obtained indicates that Chenopodium album leaves contain the highest amount of protein (9.515 ± 0.119 mg/gdw equivalent of bovine serum albumin), Chenopodium murale root contains the highest amount of carbohydrates (39.798 ± 0.105 mg/gdw equivalent of glucose) and starch (19.294 ± 0.183 mg/gdw equivalent of glucose), Chenopodium giganteum leaves contains the highest amount of flavonoids (12.397 ± 0.121 mg/gdw equivalent of quercetin), Chenopodium murale leaves contains the highest amount of phenol (4.882 ± 0.056 mg/gdw equivalent of gallic acid), among the other species. The data were analyzed by one-way ANOVA. p<0.05 and F-statistical value > F-critical value were considered the minimum significance...
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